The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Likelihood-based classification of cryo-EM images using FREALIGN
Open accessAbstract
We describe an implementation of maximum likelihood classification for single particle electron cryo-microscopy that is based on the FREALIGN software. Particle alignment parameters are determined by maximizing a joint likelihood that can include hierarchical priors, while classification is performed by expectation maximization of a marginal likelihood. We test the FREALIGN implementation using a simulated dataset containing computer-generated projection images of three different 70S ribosome structures, as well as a publicly available dataset of 70S ribosomes. The results show that the mixed strategy of the new FREALIGN algorithm yields performance on par with other maximum likelihood implementations, while remaining computationally efficient.
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Cites
RELION: Implementation of a Bayesian approach to cryo-EM structure determination
Scheres, S. H. W. (2012) · Journal of Structural Biology · doi:10.1016/j.jsb.2012.09.006 · Cited by 5,446
Rosenthal, P. B., & Henderson, R. (2003) · Journal of Molecular Biology · doi:10.1016/j.jmb.2003.07.013 · Cited by 2,416
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“The shiny stacks were exported to Frealign 9.11 98,99 and subjected to 3D classi fi cations without alignments with a 3D mask around GTPBP1.”
Influential
Metadata
- Authors
- Lyumkis, D., Brilot, A. F., Theobald, D. L., & Grigorieff, N.
- Venue
- Journal of Structural Biology
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 183
- Issue
- 3
- Pages
- 377-388
- ISSN
- 1047-8477 (ISSN-L)
- OpenAlex
- W2104382086
- Cited by
- 259
- References
- 2
- FWCI
- 13.65
- Type
- journal article
- Language
- en
- Open access
- green
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