Antioxidant peroxiredoxin 3 expression is regulated by 17beta-estradiol in rat white adipose tissue
Abstract
Peroxiredoxin 3 (PRX3) plays a role as a regulator of the adipocyte mitochondrial function due to its antioxidant activity. We have previously reported the existence of a sexual dimorphism in the mitochondrial oxidative stress status of many rat tissues such as white (WAT) and brown (BAT) adipose tissues. The aim was to elucidate whether sex hormones may play a role in PRX3 expression in the adipose tissues of rats. In in vivo experiments, male and female standard diet fed rats, high fat diet (HFD) fed rats and rosiglitazone-supplemented HFD (HDF+Rsg) fed rats, as well as ovariectomized (OVX) and 17beta-estradiol-supplemented OVX (OVX+E2) female rats were used. 3T3-L1 adipocytes and brown adipocyte primary culture were used to study the roles of both E2 and testosterone in in vitro experiments. PRX3 levels were greater in the WAT of female rats than in males. This sexual dimorphism disappeared by HFD feeding but was magnified with Rsg supplementation. PRX3 sexual dimorphism was not observed in BAT, and neither HFD nor ovariectomy modified PRX3 levels. Rsg increased Prx3 expression in the BAT of both sexes. In vitro studies supported the results obtained in vivo and confirmed the contribution of E2 to sex differences in WAT Prx3 expression. Finally, we reported an E2 upregulation of both PRX3 and thioredoxin 2 (TRX2) in WAT but not in BAT that could play a key role in the sex dimorphism reported in the antioxidant defence of WAT in order to palliate the detrimental effect of the oxidative stress.
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17β-estradiol regulates multiple other aspects of mitochondrial function through ERs, including ROS generation, antioxidant defense, and Ca2+ handling.
“Moreover, 17β-estradiol also appears to regulate multiple other aspects of mitochondrial function through ERs, including ROS generation, antioxidant defense, and Ca2+ handling (126–131).”
From From mitochondria to sarcopenia: role of 17β-estradiol and testosterone (2023) · cites this paper as [129]
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From mitochondria to sarcopenia: role of 17β-estradiol and testosterone
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Bauzá-Thorbrügge, M., Rodríguez-Cuenca, S., Vidal-Puig, A., Galmés-Pascual, B. M., Sbert-Roig, M., Gianotti, M., Lladó, I., & Proenza, A. M. (2019) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2018.09.013 · Cited by 30 · see citing works
Self-citation
- Authors
- Bauzá-Thorbrügge, M., M. Galmés-Pascual, B., Sbert-Roig, M., J. García-Palmer, F., Gianotti, M., M. Proenza, A., & Lladó, I.
- Affiliations
- Health Research Institute of the Balearic Islands (ES)
- Instituto de Salud Carlos III (ES)
- Corresponding author
- Gianotti, Magdalena
- Venue
- The Journal of Steroid Biochemistry and Molecular Biology
- Publisher
- Elsevier BV
- Published
- 2017-09
- Volume
- 172
- Pages
- 9-19
- ISSN
- 0960-0760 (ISSN-L)
- OpenAlex
- W2615203575
- Cited by
- 8
- References
- 55
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 2
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Dirección General de Investigación y Gestión del Plan Nacional de Investigación Científica (SAF2010-21792)
- Spanish Government
- Comunitat Autònoma de les Illes Balears (PCTIB-31/2011, AAEE002/2012, AAEE43/2014, AAEE52/2015)
- University of Balearic Islands (07-2014-0303240)
- Balearic Islands Government (FPI11-41523224J)
- Ministerio de Educación, Cultura y Deporte (AP2012-1004)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- peroxiredoxin 3, estradiol, white adipose tissue, brown adipose tissue, sexual dimorphism, mitochondrial oxidative stress, antioxidant defense, thioredoxin 2, testosterone, high-fat diet, rosiglitazone, ovariectomy, 3T3-L1 adipocytes
- MeSH terms
- Rosiglitazone, Animals, Antioxidants, Dietary Fats, Estradiol, Female, Gene Expression Regulation, Hypoglycemic Agents, Male, Mitochondria, Ovariectomy, Sex Characteristics, Testosterone, Thioredoxins, Signal Transduction, Rats, Wistar, Homeodomain Proteins, 3T3-L1 Cells, Thiazolidinediones, Mice, Rats, Adipose Tissue, White, Adipocytes, Brown, Adipocytes, White, Diet, High-Fat, Primary Cell Culture
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