Cited by 45Viral Infections and Immunology ResearchViral gastroenteritis research and epidemiologyVirology and Viral Diseases

Identification of vaccine-derived polioviruses using dual-stage real-time RT-PCR

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Kilpatrick, David R., Ching, Karen, Iber, Jane, Chen, Qi, Yang, Su-Ju, De, Lina, Williams, A.J., Mandelbaum, Mark +3 more·2014·Journal of Virological Methods

Abstract

Vaccine-derived polioviruses (VDPVs) are associated with polio outbreaks and prolonged infections in individuals with primary immunodeficiencies. VDPV-specific PCR assays for each of the three Sabin oral poliovirus vaccine (OPV) strains were developed, targeting sequences within the VP1 capsid region that are selected for during replication of OPV in the human intestine. Over 2400 Sabin-related isolates and identified 755 VDPVs were screened. Sensitivity of all assays was 100%, while specificity was 100% for serotypes 1 and 3, and 76% for serotype 2. The assays permit rapid, sensitive identification of OPV-related viruses and flag programmatically important isolates for further characterization by genomic sequencing.

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Authors
Kilpatrick, D. R., Ching, K., Iber, J., Chen, Q., Yang, S.-J., De, L., Williams, A. J., Mandelbaum, M., Sun, H., Oberste, M. S., & Kew, O. M.
Affiliations
Centers for Disease Control and Prevention (US)
Corresponding author
Kilpatrick, David R.
Venue
Journal of Virological Methods
Publisher
Elsevier BV
Published
2014-03
Volume
197
Pages
25-28
ISSN
0166-0934 (ISSN-L)
OpenAlex
W2022544110
Cited by
45
References
38
Citations by year
2026: 1 · 2025: 3 · 2024: 1 · 2023: 3 · 2021: 2 · 2020: 1 · 2019: 5 · 2018: 2 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 6
FWCI
4.54
Type
journal article
Language
en
Open access
Locations
  • Journal of Virological Methods (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institutes of Health (CC999999)
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
  • Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
vaccine-derived poliovirus, real-time RT-PCR, primary immunodeficiency, polio outbreak, molecular surveillance
MeSH terms
Humans, Poliomyelitis, Sensitivity and Specificity, Time Factors, Virology, Poliovirus, Poliovirus Vaccines, Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction

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