Multiplex PCR Method for Identifying Recombinant Vaccine-Related Polioviruses
Open accessAbstract
The recent discovery of recombinant circulating vaccine-derived poliovirus (recombinant cVDPV) has highlighted the need for enhanced global poliovirus surveillance to assure timely detection of any future cVDPV outbreaks. Six pairs of Sabin strain-specific recombinant primers were designed to permit rapid screening for VDPV recombinants by PCR.
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Many isolates from VAPP cases, AFP cases with incidental isolation of vaccine-related virus, and healthy OPV recipients are vaccine/vaccine recombinants.
“Many isolates from VAPP cases, AFP cases with incidental isolation of vaccine-related virus (much more frequent thanVAPPcases),andhealthyOPVrecipients are vaccine/vaccine recombinants (18, 33, 59, 91, 106).”
From VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION (2005) · cites this paper as [91]
The second new molecular approach, based upon sampling 2C and 3D sequences by PCR, can detect most vaccine/vaccine and vaccine/nonvaccine recombinants using routine procedures of the WHO Global Polio Laboratory Network.
“The second approach, based upon sampling 2C and 3D sequences by PCR, can detect most vaccine/vaccine and vaccine/nonvaccine recombinants using routine procedures of the WHO Global Polio Laboratory Network”
From VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION (2005) · cites this paper as [91]
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- Authors
- Kilpatrick, D. R., Ching, K., Iber, J., Campagnoli, R., Freeman, C. J., Mishrik, N., Liu, H.-M., Pallansch, M. A., & Kew, O. M.
- Affiliations
- Centers for Disease Control and Prevention (US)
- Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-09
- Volume
- 42
- Issue
- 9
- Pages
- 4313-4315
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC516318
- OpenAlex
- W2159602106
- Cited by
- 39
- References
- 26
- Citations by year
- 2023: 4 · 2021: 1 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 4 · 2013: 4 · 2012: 1
- FWCI
- 1.09
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Keywords
- polio surveillance, multiplex PCR, poliovirus detection, poliovirus outbreak
- MeSH terms
- Base Sequence, Vaccines, Synthetic, Polymerase Chain Reaction, DNA Primers, Poliovirus, Poliovirus Vaccines
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