Cited by 39Viral Infections and Immunology ResearchVirology and Viral DiseasesViral gastroenteritis research and epidemiology

Multiplex PCR Method for Identifying Recombinant Vaccine-Related Polioviruses

Open access
Kilpatrick, David R., Ching, Karen, Iber, Jane, Campagnoli, Ray, Freeman, Christopher J., Mishrik, Nada, Liu, Hong-Mei, Pallansch, Mark A. +1 more·2004·Journal of Clinical Microbiology

Abstract

The recent discovery of recombinant circulating vaccine-derived poliovirus (recombinant cVDPV) has highlighted the need for enhanced global poliovirus surveillance to assure timely detection of any future cVDPV outbreaks. Six pairs of Sabin strain-specific recombinant primers were designed to permit rapid screening for VDPV recombinants by PCR.

Integrity signals

Registry

Crossref · 10.1128/jcm.42.9.4313-4315.2004

Cites

26 of 26 references verified0 citation errors · 0 not checkable

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Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Many isolates from VAPP cases, AFP cases with incidental isolation of vaccine-related virus, and healthy OPV recipients are vaccine/vaccine recombinants.

    “Many isolates from VAPP cases, AFP cases with incidental isolation of vaccine-related virus (much more frequent thanVAPPcases),andhealthyOPVrecipients are vaccine/vaccine recombinants (18, 33, 59, 91, 106).”
  • The second new molecular approach, based upon sampling 2C and 3D sequences by PCR, can detect most vaccine/vaccine and vaccine/nonvaccine recombinants using routine procedures of the WHO Global Polio Laboratory Network.

    “The second approach, based upon sampling 2C and 3D sequences by PCR, can detect most vaccine/vaccine and vaccine/nonvaccine recombinants using routine procedures of the WHO Global Polio Laboratory Network”

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Authors
Kilpatrick, D. R., Ching, K., Iber, J., Campagnoli, R., Freeman, C. J., Mishrik, N., Liu, H.-M., Pallansch, M. A., & Kew, O. M.
Affiliations
  • Centers for Disease Control and Prevention (US)
  • Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia
Venue
Journal of Clinical Microbiology
Published
2004-09
Volume
42
Issue
9
Pages
4313-4315
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W2159602106
Cited by
39
References
26
Citations by year
2023: 4 · 2021: 1 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 4 · 2013: 4 · 2012: 1
FWCI
1.09
Type
journal article
Language
en
Open access
Locations
  • Journal of Clinical Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
Keywords
polio surveillance, multiplex PCR, poliovirus detection, poliovirus outbreak
MeSH terms
Base Sequence, Vaccines, Synthetic, Polymerase Chain Reaction, DNA Primers, Poliovirus, Poliovirus Vaccines

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