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Anticancer effects of clinically acceptable colchicine concentrations on human gastric cancer cell lines

Lin, Zu‐Yau, Kuo, Chao‐Hung, Wu, Deng‐Chyang, Chuang, Wan‐Long·2016·The Kaohsiung Journal of Medical Sciences
DOI 10.1016/j.kjms.2015.12.006PMC PMC11916832
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Abstract

Colchicine is a very cheap microtubule destabilizer. Because microtubules are an ideal target for anticancer drugs, the purpose of this study was to investigate whether clinically acceptable colchicine concentrations have anticancer effects on gastric cancer cells, and its possible anticancer mechanisms. Two human gastric cancer cell lines (i.e., AGS and NCI‐N87) were investigated by proliferative assay, microarray, quantitative reverse transcriptase‐polymerase chain reaction, and a nude mice study using clinically acceptable colchicine concentrations (2 ng/mL and 6 ng/mL for in vitro tests and 0.07 mg colchicine/kg/d for in vivo tests). Our results showed that colchicine had the same inhibitory effects on the proliferation of both cell lines. The antiproliferative effects of colchicine on both cell lines were achieved only at the concentration of 6 ng/mL (p < 0.0001). In both cell lines, 18 genes were consistently upregulated and 10 genes were consistently downregulated by 6 ng/mL colchicine, compared with 2 ng/mL colchicine. Among these genes, only the upregulated DUSP1 gene may contribute to the antiproliferative effects of colchicine on gastric cancer cells. The nude mice (BALB/c‐nu) experiment showed that colchicine‐treated mice after 14 days of treatment had lower increased tumor volume ratios (p = 0.0199) and tumor growth rates (p = 0.024) than the control mice. In conclusion, colchicine has potential for the palliative treatment of gastric cancer. However, the anticancer effects are achieved only at high clinically acceptable colchicine concentrations. Monitoring the colchicine plasma concentration is mandatory if this drug is applied for the palliative treatment of gastric cancer.

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Figure 1 Antiproliferative effects of colchicine on two gastric cancer cell lines—AGS and NCI‐N87. The premixed WST‐1 cell proliferation reagent was applied for investigation. The absorbance is measured at the 450‐nm wavelength (reference wavelength, 630 nm). In all experiments, 16 replicate wells were used to determine the mean and the standard deviation. The unpaired two‐tailed t test was applied for statistical analysis. Bars indicate the standard deviation (SD). (a) p = 0.5052; (b,c,e,f) p< 0.0001; and (d) p = 0.0628. The inhibitory effects of 6 ng/mL colchicine on cellular proliferation are 47.8% and 61.1% for the AGS cells and NCI‐N87 cells, respectively.
Figure 1 Antiproliferative effects of colchicine on two gastric cancer cell lines—AGS and NCI‐N87. The premixed WST‐1 cell proliferation reagent was applied for investigation. The absorbance is measured at the 450‐nm wavelength (reference wavelength, 630 nm). In all experiments, 16 replicate wells were used to determine the mean and the standard deviation. The unpaired two‐tailed t test was applied for statistical analysis. Bars indicate the standard deviation (SD). (a) p = 0.5052; (b,c,e,f) p< 0.0001; and (d) p = 0.0628. The inhibitory effects of 6 ng/mL colchicine on cellular proliferation are 47.8% and 61.1% for the AGS cells and NCI‐N87 cells, respectively.CC BY-NC-ND · PubMed Central
Figure 2 Increased tumor volume ratios in the colchicine‐treated mice and the control mice. The increased tumor volume ratio is Vx/V0 (in which Vx is the tumor volume at day × and V0 is the baseline pretreatment tumor volume). Data are expressed as the mean value ± standard error of the mean (i.e., error bars). The increased tumor volume ratios in colchicine‐treated mice after 14 days of treatment are significantly lower than in the control mice. (a) p = 0.0199, based on the unpaired t test.
Figure 2 Increased tumor volume ratios in the colchicine‐treated mice and the control mice. The increased tumor volume ratio is Vx/V0 (in which Vx is the tumor volume at day × and V0 is the baseline pretreatment tumor volume). Data are expressed as the mean value ± standard error of the mean (i.e., error bars). The increased tumor volume ratios in colchicine‐treated mice after 14 days of treatment are significantly lower than in the control mice. (a) p = 0.0199, based on the unpaired t test.CC BY-NC-ND · PubMed Central

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Research works printed but not linked: 40

  1. 10.1016/S1470-2045(13)70102-5 · DOI
  2. 10.1371/journal.pone.0060320 · DOI
  3. 10.5009/gnl.2013.7.4.385 · DOI
  4. 10.1542/peds.2006-1434 · DOI
  5. 10.1093/eurheartj/ehn608 · DOI
  6. 10.1016/j.ejim.2010.09.010 · DOI
  7. 10.3109/15563650.2010.495348 · DOI
  8. 10.1002/med.20097 · DOI · Paper page
  9. 10.1002/med.20242 · DOI
  10. 10.1007/s11095-012-0828-z · DOI · Paper page
  11. Sivakumar G., 2013, Colchicine semisynthetics: chemotherapeutics for cancer?, Curr Med Chem, 20, 892
  12. 10.1158/0008-5472.CAN-10-2429 · DOI
  13. 10.1007/BF00194404 · DOI
  14. 10.1002/j.1552-4604.1996.tb04753.x · DOI
  15. 10.1002/art.27327 · DOI
  16. 10.1016/j.lfs.2013.07.002 · DOI
  17. 10.1016/j.kjms.2015.01.008 · DOI
  18. 10.1093/annonc/mdg350 · DOI
  19. 10.1016/j.biopha.2012.02.001 · DOI
  20. 10.1016/j.biopha.2012.04.001 · DOI
  21. 10.1186/1471-2407-12-192 · DOI
  22. 10.1148/radiol.2472070363 · DOI
  23. 10.1158/0008-5472.CAN-06-3822 · DOI
  24. 10.1124/pr.107.00106 · DOI
  25. 10.1158/0008-5472.CAN-07-6157 · DOI
  26. Cheng P., 2015, Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells, Int J Mol Med, 35, 1715, 10.3892/ijmm.2015.2179 · DOI
  27. 10.1016/S0002-9440(10)62254-7 · DOI
  28. 10.1038/onc.2013.566 · DOI
  29. 10.1186/1476-4598-10-122 · DOI
  30. 10.1016/j.cyto.2012.09.013 · DOI
  31. 10.1002/bjs.5536 · DOI
  32. 10.1371/journal.pone.0085318 · DOI
  33. 10.1091/mbc.E10-08-0703 · DOI
  34. 10.1245/s10434-012-2437-9 · DOI
  35. 10.4161/cc.22853 · DOI
  36. Muller P.A., 2013, NCF2/p67phox: A novel player in the anti‐apoptotic functions of p53, Cell Cycle, 12, 14, 10.4161/cc.23173 · DOI
  37. 10.3892/or.2015.3998 · DOI
  38. 10.1186/1471-2407-11-413 · DOI
  39. 10.1002/jcb.23214 · DOI
  40. 10.1038/onc.2012.305 · DOI

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Metadata

Authors
Lin, Z., Kuo, C., Wu, D., & Chuang, W.
Venue
The Kaohsiung Journal of Medical Sciences
Publisher
Wiley
Published
2016-02
Volume
32
Issue
2
Pages
68-73
ISSN
  • 1607-551X
  • 2410-8650
DOI
10.1016/j.kjms.2015.12.006
PMC
PMC11916832
Cited by
68
References
0
Language
en
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