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Cited by 25Muscle Physiology and DisordersMuscle metabolism and nutritionNutrition and Health in Aging

Branched-Chain Amino Acid Supplementation Ameliorates Angiotensin II-Induced Skeletal Muscle Atrophy

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Yamanashi, Katsuma, Kinugawa, Shintaro, Fukushima, Arata, Kakutani, Naoya, Takada, Shingo, Obata, Yoshikuni, Nakano, Ippei, Yokota, Takashi +3 more·2020·Life Sciences
DOI 10.1016/j.lfs.2020.117593
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Abstract

Aims Sarcopenia is characterized by muscle mass and strength loss and reduced physical activity. Branched-chain amino acids (BCAAs) were recently described as an activator of protein synthesis via mammalian target of rapamycin (mTOR) signaling for muscle atrophy. In cardiovascular diseases, excessive activation of the renin-angiotensin system may induce an imbalance of protein synthesis and degradation, and this plays a crucial role in muscle atrophy. We investigated the effects of BCAAs on angiotensin II (Ang II)-induced muscle atrophy in mice.Materials and methods We administered Ang II (1000 ng/kg/min) or vehicle to 10-12-week-old male C57BL/6J mice via subcutaneous osmotic minipumps for 4 weeks with or without BCAA supplementation (3% BCAA in tap water).Key findings The skeletal muscle weight/tibial length and cross-sectional area were smaller in the Ang II mice than the vehicle mice; these changes were induced by an imbalance of protein synthesis and degradation signaling such as Akt/mTOR and MuRF-1/Atrogin-1. Compared to the Ang II mice, the mTOR signaling was significantly activated and Ang II-induced muscle atrophy was ameliorated in the Ang II + BCAA mice, and this attenuated the reduction of exercise capacity. Notably, the decrease of muscle weight/tibial length in the fast-twitch dominant muscles (e.g., the extensor digitorum longus) was significantly ameliorated compared to that in the slow-twitch dominant muscles (e.g., soleus). Histologically, the effect of BCAA was larger in fast-twitch than slow-twitch fibers, which may be related to the difference in BCAA catabolism.Significance BCAA supplementation could contribute to the prevention of skeletal muscle atrophy induced by Ang II.

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  • Amino acid complex supplementation ameliorates angiotensin II-induced muscle atrophy and increases skeletal muscle weight and gastrocnemius tissue cross-sectional area.

    “The supplementation of amino acid complex (leucine: isoleucine: valine = 2:1:1; 3% in tap water) ameliorates angiotensin II-induced muscle atrophy and increases skeletal muscle weight and gastrocnemius tissue cross-sectional area [29].”

    From Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy (2021) · cites this paper as [29]

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Authors
Yamanashi, K., Kinugawa, S., Fukushima, A., Kakutani, N., Takada, S., Obata, Y., Nakano, I., Yokota, T., Kitaura, Y., Shimomura, Y., & Anzai, T.
Affiliations
  • Hokkaido University (JP)
  • Nagoya University (JP)
Corresponding author
Kinugawa, Shintaro
ORCID
Venue
Life Sciences
Publisher
Elsevier BV
Published
2020-06
Volume
250
Pages
117593
ISSN
0024-3205 (ISSN-L)
DOI
10.1016/j.lfs.2020.117593
OpenAlex
W3013575291
Cited by
25
References
38
Citations by year
2026: 1 · 2025: 5 · 2024: 6 · 2023: 1 · 2022: 8 · 2021: 4
FWCI
1.06
Type
journal article
Language
en
Open access
Licence
Locations
  • Life Sciences (acceptedVersion)
  • PubMed (publishedVersion)
Funders
  • Japan Science and Technology Agency
  • Japan Society for the Promotion of Science (JP18H03187)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
Keywords
branched-chain amino acids, skeletal muscle atrophy, angiotensin II, sarcopenia, renin-angiotensin system, mTOR signaling, MuRF1, atrogin-1, protein synthesis and degradation, fast-twitch muscle, slow-twitch muscle, exercise capacity, BCAA catabolism
MeSH terms
Amino Acids, Branched-Chain, Angiotensin II, Animals, Blood Pressure, Echocardiography, Male, Mice, Inbred C57BL, Muscular Atrophy, Phosphorylation, Physical Conditioning, Animal, Renin-Angiotensin System, Signal Transduction, Muscle, Skeletal, Muscle Fibers, Skeletal, Mice

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