Cited by 12Mesenchymal stem cell researchLiver physiology and pathologyTissue Engineering and Regenerative Medicine

Adipose stem cells preincubated with theanine exert liver regeneration through increase of stem cell paracrine VEGF and suppression of ROS, pyroptosis as well as autophagy markers in liver damage induced by N-nitrosodiethylamine

Chen, Tung-Sheng, Lai, Yi-An, Lai, Yun-Ju, Chien, Chiang-Ting·2022·Life Sciences

Abstract

Aims Liver diseases induce a severe decrease in quality of life. Stem cell based therapy shows therapeutic potential in the treatment of liver injury. Theanine is a unique amino acid found in green tea and could confer beneficial effects on cell protection. This study investigates if protective effect on the liver by stem cells preincubated with theanine is better than that from stem cells without preincubated theanine. Methods We transplanted theanine preincubated adipose-derived stem cells (ADSC) to male Wistar rats with liver dysfunction induced by N-nitrosodiethylamine. The viability, migration and antioxidant capabilities were performed in the ADSC pre-incubated with theanine. Hepatic functional, structural and molecular assays were determined in the animals with or without theanine preincubated ADSC. Key findings Cell model revealed that ADSC preincubated with green tea theanine (T-ADSC) increased cell capabilities including viability, migration and paracrine secretion. In vivo results indicated that several pathological conditions were observed in rats with liver injury induced by DEN including structural changes and expression of pyroptosis as well as autophagy markers. The above pathological conditions were improved when the rats received both ADSC and T-ADSC treatment. Furthermore, T-ADSC showed better therapeutic effect on rats with liver injury than ADSC due to significant suppression of pyroptosis markers caspase-1 and IL-1β as well as autophagy marker LC3-II accompanied with intensive paracrine VEGF from T-ADSC. Significance Increased paracrine VEGF secretion from T-ADSC plays a crucial role in liver regeneration. A future clinical study may be designed for further verification of these experimental in vivo findings.

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Crossref · 10.1016/j.lfs.2022.120969

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Authors
Chen, T.-S., Lai, Y.-A., Lai, Y.-J., & Chien, C.-T.
Affiliations
National Taiwan Normal University (TW)
Corresponding author
  • Lai, Yun-Ju
  • Chien, Chiang-Ting
Venue
Life Sciences
Publisher
Elsevier BV
Published
2022-11
Volume
308
Pages
120969
ISSN
0024-3205 (ISSN-L)
OpenAlex
W4296269967
Cited by
12
References
35
Citations by year
2026: 2 · 2025: 4 · 2024: 3 · 2023: 2 · 2022: 1
FWCI
1.55
Type
journal article
Language
en
Open access
closed
Locations
  • Life Sciences (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Science and Technology, Taiwan (106-2320-B-003-002-MY3)
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
adipose-derived stem cells, theanine, liver regeneration, diethylnitrosamine, liver injury, pyroptosis, autophagy, reactive oxygen species, vascular endothelial growth factor, paracrine signaling, caspase-1, IL-1β, green tea, stem cell therapy
MeSH terms
Pyroptosis, Adipose Tissue, Animals, Antioxidants, Autophagy, Diethylnitrosamine, Glutamates, Liver Diseases, Liver Regeneration, Male, Quality of Life, Stem Cells, Tea, Biomarkers, Rats, Wistar, Reactive Oxygen Species, Caspases, Vascular Endothelial Growth Factor A, Rats

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