Cited by 127Mesenchymal stem cell researchTelomeres, Telomerase, and SenescencePluripotent Stem Cells Research

Antioxidants Inhibit Cell Senescence and Preserve Stemness of Adipose Tissue-Derived Stem Cells by Reducing ROS Generation during Long-Term In Vitro Expansion

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Liao, Naishun, Shi, Yingjun, Zhang, Cuilin, Zheng, Youshi, Wang, Yingchao, Zhao, Bixing, Zeng, Yongyi, Liu, Xiaolong +1 more·2019·Stem Cell Research & Therapy

Abstract

Background: Adipose tissue-derived mesenchymal stem cells (ADSCs) are promising candidates for regenerative medicine. However, long-term in vitro passaging leads to stemness loss and cell senescence of ADSCs, resulting in failure of ADSC-based therapy. Methods: In this study, ADSCs were treated with low dose of antioxidants (reduced glutathione and melatonin) with anti-aging and stem cell protection properties in the in vitro passaging, and the cell functions including stem cell senescence, cell migration, cell multidirectional differentiation potential, and ROS content were carefully analyzed. Results: We found that GSH and melatonin could maintain ADSC cell functions through reducing cell senescence and promoting cell migration, as well as by preserving stemness and multidirectional differentiation potential, through inhibiting ROS generation during long-term expansion of ADSCs. Conclusions: Our results suggested that antioxidant treatment could efficiently prevent the dysfunction and preserve cell functions of ADSCs after long-term passaging, providing a practical strategy to facilitate ADSC-based therapy.

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  • Melatonin supplementation decreases cellular senescence, preserves self-renewal, and activates antioxidant defence pathways in MSCs.

    “In this context, decreased cellular senescence, preserved self-renewal and activation of antioxidant defence pathways have all been observed after melatonin supplementation [460,461].”
  • Long-term culture of MSCs stimulates ROS generation, promoting oxidative stress-induced senescence.

    “Regarding MSCs, it is known that long term culture stimulates ROS generation, thus promoting oxidative stress-induced senescence [460].”

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Authors
Liao, N., Shi, Y., Zhang, C., Zheng, Y., Wang, Y., Zhao, B., Zeng, Y., Liu, X., & Liu, J.
Affiliations
Fujian Medical University (CN)
Corresponding author
  • Liu, Xiaolong
  • Liu, Jingfeng
Venue
Stem Cell Research & Therapy
Published
2019-10-17
Volume
10
Issue
1
Pages
306-306
ISSN
1757-6512 (ISSN-L)
OpenAlex
W2980985101
Cited by
127
References
40
Citations by year
2026: 7 · 2025: 18 · 2024: 25 · 2023: 25 · 2022: 24 · 2021: 19 · 2020: 8
FWCI
7.00
Type
journal article
Language
en
Open access
Locations
  • Stem Cell Research & Therapy (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • the National Natural Science Foundation of China (81672376)
  • the Youth Scientific Research Project of Fujian Provincial Health and Family Planning Commission (2017-1-85, 2017-2-60, 2018-1-93)
  • the Fuzhou Health and Family Planning Science and Technology Project (2017-S-wt2)
  • the Startup Fund for scientific research, Fujian Medical University (2016QH080, 2016QH081, 2018QH1198)
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
adipose-derived stem cells, cellular senescence, stemness maintenance, antioxidants, reduced glutathione, melatonin, reactive oxygen species, cell migration, multilineage differentiation, anti-aging
MeSH terms
Adipose Tissue, Animals, Antioxidants, Cell Differentiation, Cell Movement, Cells, Cultured, Glutathione, Male, Melatonin, Mice, Inbred C57BL, Stem Cells, Cellular Senescence, Reactive Oxygen Species, Stem Cell Transplantation, Regenerative Medicine, Cell Proliferation, Mice, Mesenchymal Stem Cells

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