Angiotensin I-converting enzyme inhibitory properties and SDS-PAGE of red lentil protein hydrolysates

Boye, Joyce Irene; Roufik, Samira; Pesta, Noemie; Barbana, Chockry

doi:10.1016/j.lwt.2010.01.014

Cited by 80Protein Hydrolysis and Bioactive PeptidesPeptidase Inhibition and AnalysisFood and Agricultural Sciences

Angiotensin I-converting enzyme inhibitory properties and SDS-PAGE of red lentil protein hydrolysates

Boye, Joyce Irene, Roufik, Samira, Pesta, Noemie, Barbana, Chockry·2010·LWT - Food Science and Technology

DOI 10.1016/j.lwt.2010.01.014

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Several studies have shown that over and above basic nutrition, additional health benefits may be provided by specific amino acids and bioactive peptides.
“Several studies have also shown that over and above that of basic nutrition, additional health benefits may be provided by specific amino acids and bioactive peptides (7,68 -81) .”
From Protein quality evaluation twenty years after the introduction of the protein digestibility corrected amino acid score method (2012) · cites this paper as [75]

Food-derived peptides with biological activity: from research to food applications.
Hartmann, R., & Meisel, H. (2007) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2007.01.013 · Cited by 1,102 · see citing works
Angiotensin I converting enzyme.
Erdös, E. G. (1975) · Circulation Research · doi:10.1161/01.res.36.2.247 · Cited by 555 · see citing works
A dipeptidyl carboxypeptidase that converts angiotensin I and inactivates bradykinin
Yang, H., Erdös, E. G., & Levin, Y. (1970) · Biochimica et Biophysica Acta (BBA) - Protein Structure · doi:10.1016/0005-2795(70)90017-6 · Cited by 536 · see citing works
Angiotensin I-converting enzyme inhibitory properties of whey protein digests: concentration and characterization of active peptides
Pihlanto-Leppälä, A., KOSKINEN, P., Piilola, K., Tupasela, T., & Korhonen, H. J. T. (2000) · Journal of Dairy Research · doi:10.1017/s0022029999003982 · Cited by 398 · see citing works
Characterization of inhibition and stability of soy-protein-derived angiotensin I-converting enzyme inhibitory peptides
Wu, J., & Ding, X. (2002) · Food Research International · doi:10.1016/s0963-9969(01)00131-4 · Cited by 344 · see citing works
Identification of novel antihypertensive peptides in milk fermented with Enterococcus faecalis
Quirós, A., Ramos, M., Muguerza, B., Delgado, M. A., Miguel, M., Aleixandre, A., & Recio, I. (2006) · International Dairy Journal · doi:10.1016/j.idairyj.2005.12.011 · Cited by 279 · see citing works
Optimisation and validation of an angiotensin-converting enzyme inhibition assay for the screening of bioactive peptides
Vermeirssen, V., Camp, J. V., & Verstraete, W. (2002) · Journal of Biochemical and Biophysical Methods · doi:10.1016/s0165-022x(02)00006-4 · Cited by 227 · see citing works
New antihypertensive peptides isolated from rapeseed
Marczak, E. D., Usui, H., Fujita, H., Yang, Y., Yokoo, M., Lipkowski, A. W., & Yoshikawa, M. (2003) · Peptides · doi:10.1016/s0196-9781(03)00174-8 · Cited by 223 · see citing works
Composition and Quality of Lentil (Lens culinaris Medik): A Review
Bhatty, R. S. (1988) · Canadian Institute of Food Science and Technology Journal · doi:10.1016/s0315-5463(88)70770-1 · Cited by 191 · see citing works
Comparative structural, emulsifying, and biological properties of 2 major canola proteins, cruciferin and napin.
Wu, J., & Muir, A. (2008) · Journal of Food Science · doi:10.1111/j.1750-3841.2008.00675.x · Cited by 184 · see citing works
Angiotensin I-Converting Enzyme Inhibitory Peptides Isolated from Tofuyo Fermented Soybean Food
Kuba, M., Tanaka, K., Tawata, S., Takeda, Y., & YASUDA, M. (2003) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.67.1278 · Cited by 181 · see citing works
Angiotensin-converting enzyme inhibitory activity of milk protein hydrolysates: Effect of substrate, enzyme and time of hydrolysis
Otte, J., Shalaby, S. M., Zakora, M., Pripp, A. H., & El‐Shabrawy, S. A. (2006) · International Dairy Journal · doi:10.1016/j.idairyj.2006.05.011 · Cited by 181 · see citing works
Angiotensin I-converting enzyme inhibitor derived from soy protein hydrolysate and produced by using membrane reactor
Chiang, W., Tsou, M.-J., Tsai, Z.-Y., & Tsai, T.-C. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2005.06.038 · Cited by 158 · see citing works
Performance of two commonly used angiotensin-converting enzyme inhibition assays using FA-PGG and HHL as substrates
Shalaby, S. M., Zakora, M., & Otte, J. (2006) · Journal of Dairy Research · doi:10.1017/s0022029905001639 · Cited by 148 · see citing works
Characterization of a Kunitz trypsin inhibitor with a single disulfide bridge from seeds of Inga laurina (SW.) Willd.
Macedo, M. L. R., Garcia, V. A., Freire, M. D. G. M., & Richardson, M. (2007) · Phytochemistry · doi:10.1016/j.phytochem.2007.01.024 · Cited by 128 · see citing works
Purification of angiotensin I-converting enzyme inhibitory peptides and antihypertensive effect of milk produced by protease-facilitated lactic fermentation
Chen, G., Tsai, J., & Pan, B. S. (2006) · International Dairy Journal · doi:10.1016/j.idairyj.2006.07.004 · Cited by 121 · see citing works
Antihypertensive effect of bioactive peptides produced by protease-facilitated lactic acid fermentation of milk
Tsai, J., Chen, T.-J., Pan, B. S., Gong, S.-D., & Chung, M.-Y. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.06.039 · Cited by 120 · see citing works
Effects of heat treatment and in vitro digestion on the Angiotensin converting enzyme inhibitory activity of some legume species
Akıllıoğlu, H. G., & Karakaya, S. (2009) · European Food Research and Technology · doi:10.1007/s00217-009-1133-x · Cited by 96 · see citing works
Determination of Nutritional and Bioactive Properties of Peptides in Enzymatic Pea, Chickpea, and Mung Bean Protein Hydrolysates
Aluko, R. E. (2008) · Journal of AOAC International · doi:10.1093/jaoac/91.4.947 · Cited by 91 · see citing works
Plant Food Allergens
Mills, E. N. C., Jenkins, J. A., & Shewry, P. R. (2006) · Plant Biotechnology · doi:10.1002/0470021837.ch13 · Cited by 89 · see citing works
Molecular imaging in cardiology
Wu, J. C., & Narula, J. (2007) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2007.01.009 · Cited by 81 · see citing works
The Classification, Functions and Evolutionary Relationships of Plant Proteins in Relation to Food Allergies
Shewry, P. R., Jenkins, J. A., Beaudoin, F., & Mills, E. N. C. (2003) · doi:10.1002/9780470995174.ch2 · Cited by 50 · see citing works
Soy protein hydrolysate ameliorates cardiovascular remodeling in rats with l-NAME-induced hypertension
Yang, H., Yang, S., Chen, S.-T., & Chen, J. (2008) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2007.11.004 · Cited by 35 · see citing works
Characterization of seed storage proteins of lentil (Lens culinaris M.)
Gupta, R. (1993) · Annals of Biology · Cited by 27 · see citing works

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“Several processing methods, such as dehulling, milling, cooking, soaking, germination or isoelectric precipitation, were found to increase the apparent protein content (Khattab and Arntfield, 2009; Mondor et al., 2009; Wang et al., 2009; Boye et al., 2010a; Pelgrom et al., 2013).”
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“[145] reported lentil protein concentrates produced by alkaline extraction-ultrafiltration showed greater solubility than those produced by alkaline extraction-isoelectric precipitation, with both showing the u-shaped pH-dependent profile.”
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“…extracts obtained in the present study exhibited a 461 more potent ACE-inhibitory activity compared to lentil protein hydrolysates (IC 50 = 462 0.44 mg protein/mL) (Boye et al., 2010) which indicate LSF lentil extracts as a 463 particularly promising potential to be used as functional ingredients.”
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Background
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López‐Barrios, L., Gutierrez-Uribe, J. A., & Serna-Saldivar, S. O. (2014) · Journal of Food Science · doi:10.1111/1750-3841.12365 · Cited by 177 · see citing works
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Angiotensin I-converting enzyme inhibitory properties of lentil protein hydrolysates: Determination of the kinetics of inhibition
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“Phenolic-rich lentils have the potential to reduce blood pressure due to its angiotensin I-converting enzyme (ACE) inhibitor activity (Boye et al., 2010; Hanson et al., 2014).”
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“The potential antihypertensive activity of CP, SP, and hydrolysates was assessed by the in vitro inhibition of angiotensin I-converting enzyme (ACE) based on the method of Boye et al. ( 2010 ).”
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“This value is similar to the IC 50 of the enzymatic hydrolysates of lentils protein (0.44 mg protein/mL) [123] and red bean (0.63 mg protein/mL), [91] but lower than that of fermented soybean meal (1.38 mg protein/mL).”
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“Once again, Boye et al. (2010) found that ACE-inhibitory activity of red lentil protein hydrolyzates varied as a function of protein fraction.”
Background
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Laskar, R. A., Khan, S., Deb, C. R., Tomlekova, N., Wani, M. R., Raina, A., & Amin, R. (2019) · Springer eBooks · doi:10.1007/978-3-030-23400-3_9 · Cited by 62 · see citing works
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Wan‐Mohtar, W. A. A. Q. I., Hamid, A. A., Abd‐Aziz, S., Muhamad, S. K. S., & Saari, N. (2013) · Journal of Food Science and Technology · doi:10.1007/s13197-012-0919-1 · Cited by 57 · see citing works
“(Boye et al. 2010) while the fat content of DWBS is 0.04 %.”
BackgroundInfluential
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“However, some times, glucosidase/lipase digestion could randomly help to make the bioactive peptides with anti-hypertension properties (Boye et al. 2010; Gonz alez-M uniz, Mart ın-Mart ınez, and Bonache 2016).”
BackgroundInfluential
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“This confirmed the hydrolysis and production of peptides with significant alteration in molecular size (Avramenko et al., 2013; Boye et al., 2010).”
BackgroundInfluential
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“Determination of ACE inhibition pattern To shed light on the inhibitory mechanism of the peptide of Tyr-Met-Gly-Leu-Asp-Leu-Lys on ACE, different concentrations of the ACE inhibitory peptide solution were added to each reaction mixture according to the method of Boye et al. (2010).”
Method
Protein Processing in Food and Bioproduct Manufacturing and Techniques for Analysis
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Background
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“Whether the observed changes were mediated through antioxidant((16,18,56)), antiremodelling((57)) and/or angiotensin-converting enzyme inhibitory effects remains to be determined((23,24)).”
Background
Lentil
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“Lentils possess angiotensin I-converting enzyme (ACE) inhibitory properties (Boye et al., 2010), and the beneficial impact of ACE inhibition on COVID-19 requires further investigation and consideration.”
Background
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“reported that the IC50 of red lentil protease hydrolysate for ACE was 111 ± 1 μmol/L (18).”
Background
Electrophoretic Profiles and Angiotensin I-Converting Enzyme Inhibitory Activities of Nine Varieties of Phaseolus Vulgaris Protein Hydrolysates
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“1) inhibitors have been widely used for the clinical treatment of hypertension and heart failure in humans [2].”
Method
Antihypertensive Effect of Protein Hydrolysate from Azufrado Beans in Spontaneously Hypertensive Rats
Valenzuela‐García, P., Bobadilla, N. A., Ramírez, V., León‐Villanueva, A., Lares‐Asseff, I., Valdez-Ortíz, Á., & Medina‐Godoy, S. (2016) · Cereal Chemistry · doi:10.1094/cchem-04-16-0105-fi · Cited by 13 · see citing works
Lentil consumption reduces resistance artery remodeling and restores arterial compliance in the spontaneously hypertensive rats
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Lentils (Lens culinaris, L.)
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BackgroundInfluential
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Influential
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Background
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Walters, M. E. (2019) · doi:10.22215/etd/2019-13844
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CIERMMI Women in Science Biology, Chemistry and Life Sciences Handbook T-XIV
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Marcos-Méndez, D. A., Ramírez-Higuera, A., Oliart‐Ros, R. M., & Guadarrama-Lezama, A. Y. (2021) · ECORFAN-Mexico, S.C. eBooks · doi:10.35429/h.2021.14.30.38
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Magro, A. E. A. (2019) · doi:10.47749/t/unicamp.2019.1161633
Techno-Functional Properties and Allergenicity of Mung Bean Protein Isolates from Different Countries of OriginsPreprint
Chin, T. G. J., Ruethers, T., Chan, B. A., Lopata, A. L., & Du, J. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4801950
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Golzi, M. E., Félix, M., Marchetti, L., & Lorenzo, G. (2026) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2026.113427
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Aliakbari, Z., Emam‐Djomeh, Z., & Askari, G. (2026) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2026.113349
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Yılmaz, İ., Uzun, Ş. A., Parlak, M. E., Kurt, A., & Sarıcaoğlu, F. T. (2026) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.71161

Angiotensin I-converting enzyme inhibitory properties of lentil protein hydrolysates: Determination of the kinetics of inhibition
Barbana, C., & Boye, J. I. (2011) · Food Chemistry · doi:10.1016/j.foodchem.2010.12.093 · Cited by 161 · see citing works
Self-citation
ACE-inhibitory activity of enzymatic protein hydrolysates from lupin and other legumes
Boschin, G., Scigliuolo, G. M., Resta, D., & Arnoldi, A. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.07.076 · Cited by 174 · see citing works
Purification and characterization of angiotensin I-converting enzyme inhibitory peptides of small red bean (Phaseolus vulgaris) hydrolysates
Rui, X., Boye, J. I., Simpson, B. K., & Prasher, S. O. (2013) · Journal of Functional Foods · doi:10.1016/j.jff.2013.03.008 · Cited by 95 · see citing works
Self-citation
lima bean (Phaseolus lunatus) protein hydrolysates with ACE-I inhibitory activity. Food and Nutrition Sciences
Chel-guerrero, L., Domínguez-Magaña, M. A. D. J., Ayala, A. L. M., Dávila-Ortíz, G., & Betancur‐Ancona, D. (2012)
Lima Bean (Phaseolus lunatus) Protein Hydrolysates with ACE-I Inhibitory Activity
Chel‐Guerrero, L., Domínguez-Magaña, M. A. D. J., Ayala, A. L. M., Dávila-Ortíz, G., & Betancur‐Ancona, D. (2012) · Food and Nutrition Sciences · doi:10.4236/fns.2012.34072 · Cited by 44 · see citing works

Authors: Boye, J. I., Roufik, S., Pesta, N., & Barbana, C.
Affiliations: Agriculture and Agri-Food Canada (CA)
Health Canada (CA)
Université de Reims Champagne-Ardenne (FR)
Corresponding author: Boye, Joyce Irene
Venue: LWT - Food Science and Technology
Publisher: Elsevier BV
Published: 2010-07
Volume: 43
Issue: 6
Pages: 987-991
ISSN: 0023-6438 (ISSN-L)
DOI: 10.1016/j.lwt.2010.01.014
OpenAlex: W2080057330
Cited by: 80
References: 24
Citations by year: 2026: 4 · 2025: 3 · 2024: 8 · 2023: 4 · 2022: 9 · 2021: 5 · 2020: 1 · 2019: 6 · 2018: 4 · 2017: 2 · 2016: 5 · 2015: 1 · 2014: 9 · 2013: 7 · 2012: 7
FWCI: 2.47
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: LWT (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
Health Sciences › Medicine › Oncology › Peptidase Inhibition and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
Keywords: red lentil, lentil, protein hydrolysates, angiotensin-converting enzyme inhibition, ACE inhibitory peptides, hypertension prevention, trypsin hydrolysis, legumin, vicilin, albumin, protein fractions

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Angiotensin I-converting enzyme inhibitory properties and SDS-PAGE of red lentil protein hydrolysates

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