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Cited by 23Enzyme Production and CharacterizationEnzyme Catalysis and ImmobilizationBiofuel production and bioconversion

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Starches from different sources hydrolysis using a new thermo-tolerant amylase complex produced by Bacillus subtilis T41a: Characterization and efficiency evaluation

Tavallaie, Saideh, Khomeiri, Morteza, Mousivand, Maryam, Maghsoudlou, Yahya, Hashemi, Maryam·2019·LWT
DOI 10.1016/j.lwt.2019.05.116
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Abstract

Bacillus subtilis T41a, an amylase-producing bacterium, was isolated from wheat phylosphere. The optimum catalytic activity and affinity toward different starch types hydrolysis were studied. Response surface methodology was applied to investigate the optimum catalytic conditions. The results indicated that the optimum conditions for enzyme activity were 58 °C and pH 7.2. The enzyme complex was thermostable, retaining almost 50% of its original activity after being kept at 60 °C for 1.5 h. Furthermore, the temperature stability test showed that it was stable at 40 °C for 48 h. Moreover, it was quite stable in a wide pH range of 5–9. Also, the enzyme efficiency in hydrolysing different starches was demonstrated more affinity toward potato starch. The hydrolysis rate recorded over 9 h for 10% substrate concentration of potato, corn and wheat starches were 41, 39 and 38% respectively. AFM images of hydrolyzed starches established similar pattern for potato and corn starches while different surficial morphology in case of wheat starch has been observed. Hence, the B. subtilis T41a amylase complex, as a thermo-tolerant amylase with a good ability to hydrolyze starch can be considered as an interesting and economical candidate for industrial application.

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Metadata

Authors
Tavallaie, S., Khomeiri, M., Mousivand, M., Maghsoudlou, Y., & Hashemi, M.
Affiliations
  • Gorgan University of Agricultural Sciences and Natural Resources (IR)
  • Agricultural Research & Education Organization (IR)
Corresponding author
  • Khomeiri, Morteza
  • Hashemi, Maryam
ORCID
Venue
LWT
Publisher
Elsevier BV
Published
2019-09
Volume
112
Pages
108218
ISSN
0023-6438 (ISSN-L)
DOI
10.1016/j.lwt.2019.05.116
OpenAlex
W2947595464
Cited by
23
References
0
Citations by year
2025: 3 · 2024: 1 · 2023: 15 · 2022: 1 · 2021: 3
FWCI
0.54
Type
journal article
Language
en
Open access
closed
Licence
Locations
LWT (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Keywords
amylase, starch hydrolysis, potato starch, corn starch, wheat starch, enzyme stability, enzyme optimization, starch morphology, industrial enzyme applications

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