CHL1 negatively regulates the proliferation and neuronal differentiation of neural progenitor cells through activation of the ERK1/2 MAPK pathway.
Abstract
Neural recognition molecules of the immunoglobulin superfamily play important roles in the development and regeneration of nervous system. Close Homologue of L1 (CHL1) is a member of the L1 family of recognition molecules which are expressed during neuronal development, suggesting a potential role in neural progenitor cells (NPCs). Here, we investigated the role of CHL1 in the proliferation and differentiation of NPCs both in vivo and in vitro, and the possible mechanism involved. The number of BrdU-positive cells in the subventricular zone (SVZ) significantly increased in CHL1-/- mice compared with CHL1+/+ mice. Moreover, there were more Tuj1-positive cells in the cortical plate region in CHL1-/- mice than in CHL1+/+ controls. To further examine the function of CHL1 in the proliferation and differentiation of NPCs, NPCs from CHL1-/- mice versus littermate wild-type mice were isolated and cultured in vitro. NPCs derived from CHL1-/- mice showed increased proliferation and self-renewal ability compared with CHL1+/+ mice. In the course of differentiation, CHL1 deficiency enhanced neuronal differentiation in the absence of growth factors. Furthermore, CHL1 deficiency on the proliferation of NPCs is accompanied by means of enhanced activation of ERK1/2 mitogen-activated protein kinase (MAPK) and the inhibitor of ERK1/2 MAPK eliminates the effect of CHL1 deficiency on the proliferation of NPCs. Our results first describe the negative modulation of the proliferation and neuronal differentiation of NPCs by CHL1/ERK1/2 MAPK signaling.
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CHL1 is a cell adhesion molecule from the L1 family; deletion and repetition of this gene have been linked to a number of neurological disorders, including autism, Parkinson's disease, and mental disability.
“CHL1 is a cell adhesion molecule from the L1 family. The deletion and repetition of this gene have been linked to a number of neurological disorders, including autism, Parkinson’s disease, and mental disability [50].”
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- Authors
- Huang, X., Zhu, L.-L., Zhao, T., Wu, L.-Y., Wu, K.-W., Schachner, M., Xiao, Z.-C., & Fan, M.
- Affiliations
- Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences (CN)
- Rutgers, The State University of New Jersey (US)
- Kunming Medical University (CN)
- Corresponding author
- Xiao, Zhi-Cheng
- Fan, Ming
- Venue
- Molecular and Cellular Neuroscience
- Publisher
- Elsevier BV
- Published
- 2011-01
- Volume
- 46
- Issue
- 1
- Pages
- 296-307
- ISSN
- 1044-7431 (ISSN-L)
- OpenAlex
- W1965608397
- Cited by
- 35
- References
- 41
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 3
- FWCI
- 0.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular and Cellular Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Rutgers University Community Repository (Rutgers University) (submittedVersion)
- Funders
- Natural Sciences Foundation of China (30670792, 30870799)
- National Basic Research Program of China (2006CB504100)
- Beijing Natural Science Foundation (5092023)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- CHL1, neural progenitor cells, ERK signaling, cell proliferation, neuronal differentiation, subventricular zone, cortical plate, self-renewal, immunoglobulin superfamily
- MeSH terms
- Animals, Cell Differentiation, Enzyme Activation, Enzyme Inhibitors, Female, Mice, Inbred C57BL, Pregnancy, Cell Adhesion Molecules, Mice, Knockout, Mitogen-Activated Protein Kinase 1, MAP Kinase Signaling System, Mitogen-Activated Protein Kinase 3, Cell Proliferation, Mice, Neural Stem Cells
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