The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Metformin decreases intracellular production of reactive oxygen species in aortic endothelial cells
Abstract
Beyond its antidiabetic activity justifying its use in the treatment of the type 2 diabetes, metformin (MET [dimethylguanidine, Glucophage]) has been shown to exhibit antioxidant properties in vitro, which could contribute to limit the deleterious vascular complications of diabetes. We investigated whether MET, at the pharmacological level of 10 -5 mol/L, was able to modulate intracellular production of reactive oxygen species (ROS) both in quiescent bovine aortic endothelial cells (BAECs) and in BAECs stimulated by a short incubation with high levels of glucose (30 mmol/L, 2 hours) or angiotensin II (10 -7 mol/L, 1 hour). Intracellular ROS production was measured by fluorescence of the DCF (2,7-dichlorodihydrofluorescein) probe. Our results showed that MET was able to reduce the intracellular production of ROS in both nonstimulated BAECs (-20%, P < .05) and BAEC stimulated by high levels of glucose or angiotensin II (-28% and -72%, respectively, P < .01). Experiments performed in the presence of the nicotinamide adenine dinucleotide phosphate [NAD(P)H] oxidase inhibitor apocynin or the respiratory mitochondrial chain inhibitor rotenone indicated that MET exerted its effect partly through an inhibition of the formation of ROS produced mainly by NAD(P)H oxidase and also, to a lesser extent, by the respiratory mitochondrial chain.
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“Metformin and other drugs used for T2DM, like the SGLT2 inhibitors, may also reduce hyperglycemia-induced elevated endothelial ROS, independent of negative effects on complex 1, such as via inhibition of NADPH oxidase or blocking the entry of glucose into the endothelium (32, 107–109, 187, 188)”
From A Critical Review of the Evidence That Metformin Is a Putative Anti-Aging Drug That Enhances Healthspan and Extends Lifespan (2021) · cites this paper as [107]
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1 retracted
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RetractedRole of AMP-Activated Protein Kinase in Mechanism of Metformin Action
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Research works printed but not linked: 1
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Cited by
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Cellular and molecular mechanisms of metformin: an overview
Viollet, B., Guigas, B., Garcia, N. S., Leclerc, J., Foretz, M., & Andreelli, F. (2012) · Clinical Science · doi:10.1042/cs20110386 · Cited by 1,720
Metformin: from mechanisms of action to therapies
Foretz, M., Guigas, B., Bertrand, L., Pollak, M., & Viollet, B. (2014) · Cell Metabolism · doi:10.1016/j.cmet.2014.09.018 · Cited by 1,501
A review on the role of antioxidants in the management of diabetes and its complications
Rahimi, R., Nikfar, S., Larijani, B. A., & Abdollahi, M. R. (2005) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2005.07.002 · Cited by 890
Molecular mechanism of action of metformin: old or new insights?
Rena, G., Pearson, E. R., & Sakamoto, K. (2013) · Diabetologia · doi:10.1007/s00125-013-2991-0 · Cited by 479
Metformin and inflammation: its potential Beyond glucose-lowering effect
Saisho, Y. (2015) · Endocrine, Metabolic & Immune Disorders-Drug Targets · doi:10.2174/1871530315666150316124019 · Cited by 450
Metadata
- Authors
- Ouslimani, N., Peynet, J., Bonnefont-Rousselot, D., Thérond, P., Legrand, A., & Beaudeux, J.-L.
- Corresponding author
- Beaudeux, Jean-Louis
- Venue
- Metabolism
- Publisher
- Elsevier BV
- Published
- 2005-06
- Volume
- 54
- Issue
- 6
- Pages
- 829-834
- ISSN
- 0026-0495 (ISSN-L)
- OpenAlex
- W2103676852
- Cited by
- 204
- References
- 54
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 8 · 2023: 5 · 2022: 6 · 2021: 20 · 2020: 14 · 2019: 10 · 2018: 9 · 2017: 9 · 2016: 15 · 2015: 13 · 2014: 11 · 2013: 12 · 2012: 11
- FWCI
- 4.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Keywords
- metformin, reactive oxygen species, aortic endothelial cells, antioxidant activity, NADPH oxidase, apocynin, mitochondrial respiratory chain, angiotensin II, high glucose, diabetic vascular complications, type 2 diabetes
- MeSH terms
- Animals, Aorta, Cattle, Cells, Cultured, Hypoglycemic Agents, Metformin, Reactive Oxygen Species, NADPH Oxidases, Endothelial Cells
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