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Cited by 78Natural Antidiabetic Agents StudiesMetabolism, Diabetes, and CancerMedicinal Plant Research

Bioactives of Artemisia dracunculus L enhance cellular insulin signaling in primary human skeletal muscle culture

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Wang, Zhong Q., Ribnicky, David, Zhang, Xian H., Raskin, Ilya, Yu, Yongmei, Cefalu, William T.·2008·Metabolism
DOI 10.1016/j.metabol.2008.04.003PMC PMC2981033
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Abstract

An alcoholic extract of Artemisia dracunculus L (PMI 5011) has been shown to decrease glucose and improve insulin levels in animal models, suggesting an ability to enhance insulin sensitivity. We sought to assess the cellular mechanism by which this botanical affects carbohydrate metabolism in primary human skeletal muscle culture. We measured basal and insulin-stimulated glucose uptake, glycogen accumulation, phosphoinositide 3 (PI-3) kinase activity, and Akt phosphorylation in primary skeletal muscle culture from subjects with type 2 diabetes mellitus incubated with or without various concentrations of PMI 5011. We also analyzed the abundance of insulin receptor signaling proteins, for example, IRS-1, IRS-2, and PI-3 kinase. Glucose uptake was significantly increased in the presence of increasing concentrations of PMI 5011. In addition, glycogen accumulation, observed to be decreased with increasing free fatty acid levels, was partially restored with PMI 5011. PMI 5011 treatment did not appear to significantly affect protein abundance for IRS-1, IRS-2, PI-3 kinase, Akt, insulin receptor, or Glut-4. However, PMI 5011 significantly decreased levels of a specific protein tyrosine phosphatase, that is, PTP1B. Time course studies confirmed that protein abundance of PTP1B decreases in the presence of PMI 5011. The cellular mechanism of action to explain the effects by which an alcoholic extract of A dracunculus L improves carbohydrate metabolism on a clinical level may be secondary to enhancing insulin receptor signaling and modulating levels of a specific protein tyrosine phosphatase, that is, PTP1B.

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  • Russian tarragon appears to have antihyperglycemic activity when combined with CM ingestion.

    “Studies have shown that Russian tarragon (RT) appears to have antihyperglycemic activity when combined with CM ingestion (Jäger et al. 2008a; Wang et al. 2008).”

    From Analysis of the efficacy, safety, and regulatory status of novel forms of creatine (2011) · cites this paper as Wang et al. 2008

    The Introduction reports that Russian tarragon extract lowered blood glucose in diabetic mice, and the Results report increased glucose uptake in cultured human skeletal muscle cells. Neither section addresses its effect when combined with CM ingestion, so the combination-specific claim is unsupported by this paper. (LLM, conf=0.89)

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Authors
Wang, Z. Q., Ribnicky, D., Zhang, X. H., Raskin, I., Yu, Y., & Cefalu, W. T.
Affiliations
  • Pennington Biomedical Research Center (US)
  • Rutgers, The State University of New Jersey (US)
Corresponding author
Cefalu, William T.
ORCID
Venue
Metabolism
Publisher
Elsevier BV
Published
2008-07
Volume
57
Issue
7 Suppl 1
Pages
S58-S64
ISSN
0026-0495 (ISSN-L)
DOI
10.1016/j.metabol.2008.04.003
PMC
PMC2981033
OpenAlex
W2075054243
Cited by
78
References
35
Citations by year
2026: 2 · 2023: 4 · 2022: 1 · 2021: 3 · 2020: 5 · 2019: 7 · 2018: 5 · 2016: 3 · 2015: 5 · 2014: 10 · 2013: 3 · 2012: 12
FWCI
4.15
Type
journal article
Language
en
Open access
Licence
Locations
  • Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • Rutgers University Community Repository (Rutgers University) (submittedVersion)
  • Rutgers University Community Repository (Rutgers University) (submittedVersion, other-oa)
  • Rutgers University Community Repository (Rutgers University)
Funders
National Center for Complementary and Integrative Health (P50AT002776)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
Keywords
Artemisia dracunculus, alcoholic plant extract, insulin signaling, skeletal muscle, insulin resistance, type 2 diabetes, glucose uptake, glycogen accumulation, PI3-kinase, Akt phosphorylation, IRS-1, insulin receptor substrate 2, PTP1B, protein tyrosine phosphatase, carbohydrate metabolism, free fatty acids
MeSH terms
Cells, Cultured, Diabetes Mellitus, Type 2, Drug Evaluation, Preclinical, Glucose, Glycogen, Glycogen Synthase, Humans, Insulin, Male, Middle Aged, Models, Biological, Obesity, Plant Extracts, Signal Transduction, Gene Expression Regulation, Enzymologic, Muscle, Skeletal, Cell Culture Techniques, Artemisia, Phosphatidylinositol 3-Kinases

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