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Cited by 104Pancreatic function and diabetesDiabetes Treatment and ManagementAldose Reductase and Taurine

The DOI matches its Crossref record; 37 of the 38 references it cites were found in a registry or an index.

The bile acid TUDCA increases glucose-induced insulin secretion via the cAMP/PKA pathway in pancreatic beta cells

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Vettorazzi, Jean Franciesco, Ribeiro, Rosane Aparecida, Borck, Patricia Cristine, Branco, Renato Chaves Souto, Soriano, Sergi, Merino, Beatriz, Boschero, Antônio Carlos, Nadal, Angel, Quesada, Ivan, Carneiro, Everardo Magalhães·2016·Metabolism
DOI 10.1016/j.metabol.2015.10.021
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Abstract

Objective While bile acids are important for the digestion process, they also act as signaling molecules in many tissues, including the endocrine pancreas, which expresses specific bile acid receptors that regulate several cell functions. In this study, we investigated the effects of the conjugated bile acid TUDCA on glucose-stimulated insulin secretion (GSIS) from pancreatic β-cells.Methods Pancreatic islets were isolated from 90-day-old male mice. Insulin secretion was measured by radioimmunoassay, protein phosphorylation by western blot, Ca(2+) signals by fluorescence microscopy and ATP-dependent K(+) (KATP) channels by electrophysiology.Results TUDCA dose-dependently increased GSIS in fresh islets at stimulatory glucose concentrations but remained without effect at low glucose levels. This effect was not associated with changes in glucose metabolism, Ca(2+) signals or KATP channel activity; however, it was lost in the presence of a cAMP competitor or a PKA inhibitor. Additionally, PKA and CREB phosphorylation were observed after 1-hour incubation with TUDCA. The potentiation of GSIS was blunted by the Gα stimulatory, G protein subunit-specific inhibitor NF449 and mimicked by the specific TGR5 agonist INT-777, pointing to the involvement of the bile acid G protein-coupled receptor TGR5.Conclusion Our data indicate that TUDCA potentiates GSIS through the cAMP/PKA pathway.

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Metadata

Authors
Vettorazzi, J. F., Ribeiro, R. A., Borck, P. C., Branco, R. C. S., Soriano, S., Merino, B., Boschero, A. C., Nadal, A., Quesada, I., & Carneiro, E. M.
Affiliations
  • Universitat de Miguel Hernández d'Elx (ES)
  • Universidade Federal do Rio de Janeiro (BR)
  • Universidade Estadual de Campinas (UNICAMP) (BR)
  • University of Alicante (ES)
Corresponding author
Carneiro, Everardo Magalhães
ORCID
Venue
Metabolism
Publisher
Elsevier BV
Published
2016-03
Volume
65
Issue
3
Pages
54-63
ISSN
0026-0495 (ISSN-L)
DOI
10.1016/j.metabol.2015.10.021
OpenAlex
W2186068515
Cited by
104
References
38
Citations by year
2026: 7 · 2025: 8 · 2024: 14 · 2023: 13 · 2022: 9 · 2021: 10 · 2020: 13 · 2019: 10 · 2018: 7 · 2017: 8 · 2016: 5
FWCI
6.71
Type
journal article
Language
en
Open access
Licence
Locations
  • Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-nd)
  • Repositorio Institucional de la Universidad de Alicante (Universidad de Alicante) (submittedVersion)
Funders
  • Fundacão de Amparo á Pesquisa do Estado de São Paulo (FAPESP 2013/01318-4)
  • Conselho Nacional para o Desenvolvimento Científico e Tecnológico (CNPq 200030/2014-0)
  • Instituto Nacional de Obesidade e Diabetes (CNPq/FAPESP)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
  • Spanish Ministerio de Ciencia e Innovación (BFU2013-42789-P, BFU2011-28358)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Aldose Reductase and Taurine
Keywords
tauroursodeoxycholic acid, bile acids, glucose-stimulated insulin secretion, pancreatic beta cells, cAMP/PKA signaling, TGR5, G protein-coupled receptors, CREB phosphorylation, pancreatic islets, insulin secretion
MeSH terms
Cyclic AMP, Animals, Dose-Response Relationship, Drug, Glucose, Insulin, Male, Mice, Inbred C57BL, Phosphorylation, Taurochenodeoxycholic Acid, Signal Transduction, Cyclic AMP Response Element-Binding Protein, Cyclic AMP-Dependent Protein Kinases, Calcium Signaling, Receptors, G-Protein-Coupled, Protein Kinase Inhibitors, Insulin-Secreting Cells, Mice, KATP Channels, In Vitro Techniques

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