Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells

The diverse functions of GAPDH: Views from different subcellular compartments

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Where the endoplasmic reticulum and the mitochondrion tie the knot: The mitochondria-associated membrane (MAM)

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Isolated heart perfusion according to Langendorff—Still viable in the new millennium

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Differential detergent sensitivity of extracellular vesicle subpopulations

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Crude subcellular fractionation of cultured mammalian cell lines

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[19] Permeabilizing cells: Some methods and applications for the study of intracellular processes

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Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families

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An optimised direct lysis method for gene expression studies on low cell numbers

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TDP-43 gains function due to perturbed autoregulation in a Tardbp knock-in mouse model of ALS-FTD

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Mitochondrial NADP(H) generation is essential for proline biosynthesis

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Melatonin ameliorates H2O2-induced oxidative stress through modulation of Erk/Akt/NFkB pathway

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Subcellular proteomics

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Anti-tumor immunity in mismatch repair-deficient colorectal cancers requires type I IFN–driven CCL5 and CXCL10

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The phase separation-dependent FUS interactome reveals nuclear and cytoplasmic function of liquid–liquid phase separation

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SOAT1 promotes mevalonate pathway dependency in pancreatic cancer

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Rosmarinic Acid Attenuates Cadmium-Induced Nephrotoxicity via Inhibition of Oxidative Stress, Apoptosis, Inflammation and Fibrosis

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Protocatechuic Acid, a Phenolic from Sansevieria roxburghiana Leaves, Suppresses Diabetic Cardiomyopathy via Stimulating Glucose Metabolism, Ameliorating Oxidative Stress, and Inhibiting Inflammation

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Wheat phenolics suppress doxorubicin-induced cardiotoxicity via inhibition of oxidative stress, MAP kinase activation, NF-κB pathway, PI3K/Akt/mTOR impairment, and cardiac apoptosis

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Western Blotting: A Powerful Staple In Scientific and Biomedical Research

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Diurnal Expression of PD-1 on Tumor-Associated Macrophages Underlies the Dosing Time-Dependent Antitumor Effects of the PD-1/PD-L1 Inhibitor BMS-1 in B16/BL6 Melanoma-Bearing Mice

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Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response

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CHCHD2 and CHCHD10 regulate mitochondrial dynamics and integrated stress response

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MIR503HG Loss Promotes Endothelial-to-Mesenchymal Transition in Vascular Disease

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Therapeutic potential of vanadium complexes with 1,10-phenanthroline ligands, quo vadis? Fate of complexes in cell media and cancer cells

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Simple and Efficient Protocol for Subcellular Fractionation of Normal and Apoptotic Cells

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Synergistic antitumor interaction of valproic acid and simvastatin sensitizes prostate cancer to docetaxel by targeting CSCs compartment via YAP inhibition

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Brain clusterin protein isoforms and mitochondrial localization

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Aptamer-Functionalized Drug Nanocarrier Improves Hepatocellular Carcinoma toward Normal by Targeting Neoplastic Hepatocytes

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A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes

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Gasdermin D Mediates Inflammation-Induced Defects in Reverse Cholesterol Transport and Promotes Atherosclerosis

Opoku, E., Traughber, C. A., Zhang, D. K. Y., Iacano, A. J., Khan, M. R., Han, J., Smith, J. D., & Gulshan, K. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.715211 · Cited by 53 · see citing works

Quantification of autophagy flux using LC3 ELISA

Oh, S.-H., Choi, Y.-B., Kim, J.-H., Weihl, C. C., & Ju, J.-S. (2017) · Analytical Biochemistry · doi:10.1016/j.ab.2017.05.003 · Cited by 53 · see citing works

A SUMOylation-dependent HIF-1α/CLDN6 negative feedback mitigates hypoxia-induced breast cancer metastasis

Jia, Y., Guo, Y., Qiu, J., Qu, H., Qi, D., Song, P., Zhang, X., Wang, X., Xu, W., Dong, Y., et al. (2020) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-020-01547-5 · Cited by 52 · see citing works

SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles

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Edible leaf extract of Ipomoea aquatica Forssk. (Convolvulaceae) attenuates doxorubicin-induced liver injury via inhibiting oxidative impairment, MAPK activation and intrinsic pathway of apoptosis

Dewanjee, S., Joardar, S., Bhattacharjee, N., Dua, T. K., Das, S., Kalita, J., & Manna, P. (2017) · Food and Chemical Toxicology · doi:10.1016/j.fct.2017.05.002 · Cited by 43 · see citing works

Carnosic Acid, a Natural Diterpene, Attenuates Arsenic‐Induced Hepatotoxicity via Reducing Oxidative Stress, MAPK Activation, and Apoptotic Cell Death Pathway

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STING protects breast cancer cells from intrinsic and genotoxic-induced DNA instability via a non-canonical, cell-autonomous pathway

Cheradame, L., Guerrera, I. C., Gaston, J., Schmitt, A., Jung, V. A., Goudin, N., Pouillard, M., Radosevic‐Robin, N., Modesti, M., Judde, J., et al. (2021) · Oncogene · doi:10.1038/s41388-021-02037-4 · Cited by 47 · see citing works

Non‐psychotropic Cannabis sativa L. phytocomplex modulates microglial inflammatory response through CB2 receptors‐, endocannabinoids‐, and NF‐κB ‐mediated signaling

Borgonetti, V., Benatti, C., Governa, P., Isoldi, G., Pellati, F., Alboni, S., Tascedda, F., Montopoli, M., Galeotti, N., Manetti, F., et al. (2022) · Phytotherapy Research · doi:10.1002/ptr.7458 · Cited by 42 · see citing works

Tumor factors stimulate lysosomal degradation of tumor antigens and undermine their cross-presentation in lung cancer

Lu, Z. F., Chen, J., Yu, P., Atherton, M. J., Gui, J., Tomar, V. S., Middleton, J. D., Sullivan, N. T., Singhal, S. K., George, S. S., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-34428-w · Cited by 38 · see citing works

Rhodiola rosea L. modulates inflammatory processes in a CRH-activated BV2 cell model

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ADAM10 Plasma and CSF Levels Are Increased in Mild Alzheimer’s Disease

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Cytoplasmic Endonuclease G promotes nonalcoholic fatty liver disease via mTORC2-AKT-ACLY and endoplasmic reticulum stress

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Nuclear matrix metalloproteinase-2 in the cardiomyocyte and the ischemic-reperfused heart

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An Albumin-binding Polypeptide Both Targets Cytotoxic T Lymphocyte Vaccines to Lymph Nodes and Boosts Vaccine Presentation by Dendritic Cells

Wang, P., Zhao, P., Dong, S., Xu, T., He, X., & Chen, M. (2017) · Theranostics · doi:10.7150/thno.21691 · Cited by 37 · see citing works

Modulation of TLR4 Sialylation Mediated by a Sialidase Neu1 and Impairment of Its Signaling in Leishmania donovani Infected Macrophages

Karmakar, J., Roy, S., & Mandal, C. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.02360 · Cited by 37 · see citing works

Phosphoregulation of the oncogenic protein regulator of cytokinesis 1 (PRC1) by the atypical CDK16/CCNY complex

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EGFR phosphorylates HDAC1 to regulate its expression and anti-apoptotic function

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Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion

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Challenges with Methods for Detecting and Studying the Transcription Factor Nuclear Factor Kappa B (NF-κB) in the Central Nervous System

Mostafizar, M., Cortes-Pérez, C., Snow, W., Djordjevic, J., Adlimoghaddam, A., & Albensi, B. (2021) · Cells · doi:10.3390/cells10061335 · Cited by 30 · see citing works

Biomimetic liposomes in drug delivery: from design mechanisms to applications

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ADAR and hnRNPC deficiency synergize in activating endogenous dsRNA-induced type I IFN responses

Herzner, A., Khan, Z., Nostrand, E. L. V., Chan, S. M., Cuellar, T. L., Chen, R., Pechuan-Jorge, X., Kömüves, L. G., Solon, M., Modrusan, Z. D., et al. (2021) · The Journal of Experimental Medicine · doi:10.1084/jem.20201833 · Cited by 34 · see citing works

Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells

Sharma, A., Ravi, V., Venkataswamy, M. M., & Desai, A. S. (2020) · Journal of Biomedical Science · doi:10.1186/s12929-020-00639-w · Cited by 33 · see citing works

Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium

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Catalase deficiency induces reactive oxygen species mediated pexophagy and cell death in the liver during prolonged fasting

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KChIP2 is a core transcriptional regulator of cardiac excitability

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α-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation

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Oncogenic KRAS engages an RSK1/NF1 pathway to inhibit wild-type RAS signaling in pancreatic cancer

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Anaphylactic degranulation by mast cells requires the mobilization of inflammasome components

Mencarelli, A., Bist, P., Choi, H. W., Khameneh, H. J., Mortellaro, A., & Abraham, S. N. (2024) · Nature Immunology · doi:10.1038/s41590-024-01788-y · Cited by 29 · see citing works

The effect of Mexidol on cerebral mitochondriogenesis at a young age and during aging

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Enhancing calmodulin binding to ryanodine receptor is crucial to limit neuronal cell loss in Alzheimer disease

Nakamura, Y., Yamamoto, T., Xu, X., Kobayashi, S., Tanaka, S., Tamitani, M., Saito, T., Saido, T. C., & Yano, M. (2021) · Scientific Reports · doi:10.1038/s41598-021-86822-x · Cited by 28 · see citing works

The dual fates of exogenous tau seeds: Lysosomal clearance versus cytoplasmic amplification

Kolay, S., Vega, A. R., Dodd, D. A., Perez, V. A., Kashmer, O. M., White, C. L., & Diamond, M. I. (2022) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2022.102014 · Cited by 29 · see citing works

Sansevieria roxburghiana Schult. & Schult. F. (Family: Asparagaceae) Attenuates Type 2 Diabetes and Its Associated Cardiomyopathy

Bhattacharjee, N., Khanra, R., Dua, T. K., Das, S., De, B., Zia‐Ul‐Haq, M., Feo, V. D., & Dewanjee, S. (2016) · PLoS ONE · doi:10.1371/journal.pone.0167131 · Cited by 28 · see citing works

Ochratoxin A induces epithelial-to-mesenchymal transition and renal fibrosis through TGF-β/Smad2/3 and Wnt1/β-catenin signaling pathways in vitro and in vivo

Pyo, M. C., Chae, S. A., Yoo, H. J., & Lee, K. (2020) · Archives of Toxicology · doi:10.1007/s00204-020-02829-9 · Cited by 26 · see citing works

Tumor-Suppressive and Immune-Stimulating Roles of Cholesterol 25-hydroxylase in Pancreatic Cancer Cells

McBrearty, N., Cho, C., Chen, J., Zahedi, F., Peck, A. R., Radaelli, E., Assenmacher, C., Pavlak, C., Devine, A., Yu, P., et al. (2022) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-22-0602 · Cited by 24 · see citing works

New insights into the analgesic properties of the XCL1/XCR1 and XCL1/ITGA9 axes modulation under neuropathic pain conditions - evidence from animal studies

Ciechanowska, A., Rojewska, E., Piotrowska, A., Barut, J., Pawlik, K., Ciapała, K., Kreiner, G., & Mika, J. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.1058204 · Cited by 24 · see citing works

Selective killing of proinflammatory synovial fibroblasts via activation of transient receptor potential ankyrin (TRPA1)

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Nuclear and Cytoplasmatic Quantification of Unconjugated, Label-Free Locked Nucleic Acid Oligonucleotides

Pendergraff, H. M., Schmidt, S., Vikeså, J., Weile, C., Øverup, C., Lindholm, M., & Koch, T. (2019) · Nucleic Acid Therapeutics · doi:10.1089/nat.2019.0810 · Cited by 24 · see citing works

Sex-specific deficits in biochemical but not behavioral responses to delay fear conditioning in prenatal alcohol exposure mice

Caldwell, K. K., Solomon, E. R., Smoake, J., de Kamgaing, C. D. D., & Allan, A. M. (2018) · Neurobiology of Learning and Memory · doi:10.1016/j.nlm.2018.10.002 · Cited by 25 · see citing works

Neurotensin receptor 2 is induced in astrocytes and brain endothelial cells in relation to neuroinflammation following pilocarpine‐induced seizures in rats

Kyriatzis, G., Bernard, A., Bôle, A., Pflieger, G., Chalas, P., Masse, M., Lécorché, P., Jacquot, G., Ferhat, L., & Khrestchatisky, M. (2021) · Glia · doi:10.1002/glia.24062 · Cited by 17 · see citing works

Carnosic acid attenuates doxorubicin-induced cardiotoxicity by decreasing oxidative stress and its concomitant pathological consequences

Manna, P., Dewanjee, S., Joardar, S., Chakraborty, P., Bhattacharya, H., Bhanja, S., Bhattacharyya, C., Bhowmik, M., Bhowmick, S., Saha, A., et al. (2022) · Food and Chemical Toxicology · doi:10.1016/j.fct.2022.113205 · Cited by 23 · see citing works

Harnessing PROTAC technology to combat stress hormone receptor activation

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Gingipain-carrying outer membrane vesicles from Porphyromonas gingivalis cause barrier dysfunction of Caco-2 cells by releasing gingipain into the cytosol

Nonaka, S., Okamoto, R., Katsuta, Y., Kanetsuki, S., & Nakanishi, H. (2024) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2024.149783 · Cited by 23 · see citing works

Sex-Dependent Effects of the Histone Deacetylase Inhibitor, Sodium Valproate, on Reversal Learning After Developmental Arsenic Exposure

Steadman, C. R., Smoake, J., Solomon, E. R., Villicana, E., Caldwell, K. K., & Allan, A. M. (2018) · Frontiers in Genetics · doi:10.3389/fgene.2018.00200 · Cited by 23 · see citing works

Kaempferol Inhibits Extra-synaptic NMDAR-Mediated Downregulation of TRkβ in Rat Hippocampus During Hypoxia

Das, D., Biswal, S., Barhwal, K. K., Chaurasia, O. P., & Hota, S. K. (2018) · Neuroscience · doi:10.1016/j.neuroscience.2018.09.018 · Cited by 23 · see citing works

WIN 55,212-2 Reverted Pilocarpine-Induced Status Epilepticus Early Changes of the Interaction among 5-HT2C/NMDA/CB1 Receptors in the Rat Hippocampus

Maio, R. D., Colangeli, R., & Giovanni, G. D. (2019) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.9b00080 · Cited by 23 · see citing works

Correlation between in vitro binding activity of sweeteners to cloned human sweet taste receptor and sensory evaluation

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The linker domain of the SNARE protein SNAP25 acts as a flexible molecular spacer that ensures efficient S-acylation

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Nuclear proteasomes buffer cytoplasmic proteins during autophagy compromise

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Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction

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