Use of extracellular polysaccharides, secreted by Lactobacillus plantarum and Bacillus spp., as reducing indole production agents to control biofilm formation and efflux pumps inhibitor in Escherichia coli
Abstract
The overuse of antibiotics and biofilm formation ability has led to the emergence of bacterial resistant strains. The combined use of several antibiotics has been found as an efficient strategy to overcome this resistance. In this study, two exopolysaccharides (EPS) obtained from Lactobacillus plantarum (EPS-Lp) and Bacillus spp. (EPS-B), isolated from a traditional Tunisian food "ricotta cheese" and hypersaline environment respectively, were used to counteract the biofilm formation and efflux pumps activities in Escherichia coli ATCC35218. The obtained results revealed that the tested EPSs can be effective against E. coli at a concentration > 1 mg/ml and were able to modulate biofilm formation by 50%. Moreover, at a concentration of 512 μg/ml, the tested EPSs inhibit the EtBr efflux in the tested bacteria and no significant difference was shown compared to cells treated with reserpine (P > 0.05). The positive effect of the tested EPSs may be due to the decrease of Indole production level proposed as a signal involved in quorum sensing and through the significant reduction of the hydrophobicity percentage between the treated and untreated cells. Overall, EPS-Lp and EPS-B, when used at appropriate concentration, may inhibit biofilm formation and reduce efflux pumps implicated in bacterial adhesion and antimicrobial resistance. These results make them an interesting candidate in the design of a new strategies to control bacterial biofilm-associated infections.
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EPSs isolated from L. plantarum can reduce biofilm production by inhibiting indole production associated with quorum sensing, as shown in Escherichia coli.
“EPSs isolated from L. plantarum can also reduce biofilm production by inhibiting indole production associated with quorum sensing, as shown in Escherichia coli [18].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [18]
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<p>The Battle of Probiotics and Their Derivatives Against Biofilms</p>
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“plantarum can also reduce biofilm production by inhibiting indole production associated with quorum sensing, as shown in Escherichia coli [18].”
Background
- Authors
- Mahdhi, A., Leban, N., Chakroun, I., Bayar, S., Mahdouani, K., Majdoub, H., & Kouidhi, B.
- Affiliations
- University of Monastir (TN)
- Taibah University (SA)
- Corresponding author
- Kouidhi, Bochra
- Venue
- Microbial Pathogenesis
- Publisher
- Elsevier BV
- Published
- 2018-12
- Volume
- 125
- Pages
- 448-453
- ISSN
- 0882-4010 (ISSN-L)
- OpenAlex
- W2896326261
- Cited by
- 54
- References
- 37
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 4 · 2023: 9 · 2022: 7 · 2021: 11 · 2020: 8 · 2019: 1
- FWCI
- 1.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Microbial Pathogenesis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Keywords
- exopolysaccharides, Lactobacillus plantarum, Bacillus, Escherichia coli, biofilm formation, efflux pumps, indole production, quorum sensing, antimicrobial resistance, ricotta cheese, hypersaline environments, reserpine, hydrophobicity, biofilm-associated infections
- MeSH terms
- Anti-Bacterial Agents, Bacillus, Biological Transport, Active, Environmental Microbiology, Escherichia coli, Food Microbiology, Indoles, Microbial Sensitivity Tests, Polysaccharides, Bacterial, Biofilms, Lactiplantibacillus plantarum, Quorum Sensing, Hydrophobic and Hydrophilic Interactions
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