Immunomodulatory mechanism of Bacillus subtilis R0179 in RAW 264.7 cells against Candida albicans challenge
Abstract
This study was aimed to explore the immunomodulatory and anti-Candida mechanisms of Bacillus subtilis (B. subtilis) R0179 in macrophages. RAW 264.7 cells were first challenged with B. subtilis R0179. B. subtilis R0179 was found to down-regulate the signals of Dectin-1, Card9, P-Iκ-Bα, Iκ-Bα, and NF-κB. Meanwhile, it reduced the levels of cytokines interleukin (IL)-1β, IL-6, IL-12, and tumor necrosis factor (TNF)-α, but increased the level of cytokine IL-10. Then RAW 264.7 cells were pretreated with B. subtilis R0179 before challenged with Candida albicans (C. albicans) or RAW 264.7 cells were co-treated with B. subtilis R0179 and C. albicans. In the presence of C. albicans, B. subtilis R0179 also showed the similar immunomodulatory effects on RAW 264.7 cells. Hence, this study provides the first insight into the immunomodulatory mechanisms of B. subtilis R0179 on the Dectin-1-related downstream signaling pathways in macrophages, which may prevent tissue damage caused by excessive pro-inflammatory response during the infection of C. albicans.
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The discharge of anti-inflammatory cytokines IL-4 or IL-10 can control the progression of sepsis.
“the discharge of anti-inflammatory cytokines IL-4 [19] or IL-10 [12,20,21] can control the progression of sepsis.”
From The F <sub>1</sub> F <sub>o</sub> -ATP synthase α subunit of <i>Candida albicans</i> induces inflammatory responses by controlling amino acid catabolism (2023) · cites this paper as [20]
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- Authors
- Zhao, W., Wang, X., Zhao, C., & Yan, Z.
- Affiliations
- Peking University (CN)
- Corresponding author
- Yan, Zhimin
- Venue
- Microbial Pathogenesis
- Publisher
- Elsevier BV
- Published
- 2021-08
- Volume
- 157
- Pages
- 104988
- ISSN
- 0882-4010 (ISSN-L)
- OpenAlex
- W3164915648
- Cited by
- 9
- References
- 57
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 2
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Microbial Pathogenesis (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Keywords
- Candida albicans, immunomodulation, macrophages, RAW 264.7 macrophages, CARD9, NF-κB signaling, inflammatory cytokines, IL-10, antifungal immunity, pro-inflammatory response
- MeSH terms
- RAW 264.7 Cells, Animals, Bacillus subtilis, Candida albicans, Immunity, Tumor Necrosis Factor-alpha, NF-kappa B, Mice, CARD Signaling Adaptor Proteins
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