CoChat
Sign Up
Cited by 123Marine Sponges and Natural ProductsMicrobial Natural Products and BiosynthesisMicrobial Community Ecology and Physiology

Isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities

Open access
Romanenko, Lyudmila A., Uchino, Masataka, Kalinovskaya, Natalia I., Mikhailov, Valery V.·2008·Microbiological Research
DOI 10.1016/j.micres.2006.10.001
Open this paper in CoChat — free account

Abstract

This study was undertaken to survey culturable heterotrophic bacteria associated with the marine ark shell Anadara broughtoni inhabiting in the Sea of Japan, and to test isolates for their antimicrobial, hemolytic and surface activities with an emphasis on low-molecular-weight metabolites search. A total of 149 strains were isolated and identified phenotypically. A total of 27 strains were selected to be investigated phylogenetically by 165 rRNA gene sequence analysis. The most bacteria were affiliated with members of the Gammaproteobacteria and Alphaproteobacteria, and Less with Firmicutes, Actinobacteria, and Cytophaga-Flavobacterium-Bacteroides (CFB) group. The isolates capable of hemolysis were numerically abundant in the genera Pseudoalteromonas, Aeromonas and Bacillus. The six Gram-positive isolates belonging to the genera Bacillus, Paenibacillus and Saccharothrix and two Gram-negative strains related to Pseudomonas and Sphingomonas, possessed antimicrobial activity against indicator strains and to each other. Antimicrobial, hemolytic and surface activities were revealed in butanot extracts of cells or cell-free supernatant of six active strains. This points to availability of active low-molecular-weight metabolites. Substances with hemolytic and surface activities were isolated from strain Bacillus pumilus An 112 and characterized as cyclic depsipeptides with molecular masses 1021, 1035, 1049, 1063 and 1077 Da. The recovery of strains producing antimicrobial and surface-active substances suggests that microorganisms associated with the marine bivalve are potential source of bioactive metabolites.

Integrity signals

Registry

Crossref · 10.1016/j.micres.2006.10.001

Cites

37 of 37 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Marine Pseudomonas-derived bioactive substances are diverse and include pyrroles, pseudopeptides, pyrrolidinedione, phloroglucinol, phenazine, benzaldehyde, quinoline, quinolone, phenanthrene, phthalate, andrimid, moiramides, zafrin and bushrin.

    “Marine Pseudomonas-derived bioactive substances are diverse and include pyrroles, pseudopeptides, pyrrolidinedione, phloroglucinol, phenazine, benzaldehyde, quinoline, quinolone, phenanthrene, phthalate, andrimid, moiramides, zafrin and bushrin [108].”

    From Marine Natural Products: A Source of Novel Anticancer Drugs (2019) · cites this paper as [108]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Romanenko, L. A., Uchino, M., Kalinovskaya, N. I., & Mikhailov, V. V.
Affiliations
  • Russian Academy of Sciences (RU)
  • Tokyo University of Agriculture (JP)
Corresponding author
Romanenko, Lyudmila A.
ORCID
Romanenko, Lyudmila A.
Venue
Microbiological Research
Publisher
Elsevier BV
Published
2008-11
Volume
163
Issue
6
Pages
633-644
ISSN
0944-5013 (ISSN-L)
DOI
10.1016/j.micres.2006.10.001
OpenAlex
W2011043474
Cited by
123
References
37
Citations by year
2025: 3 · 2024: 6 · 2023: 9 · 2022: 7 · 2021: 6 · 2020: 4 · 2019: 13 · 2018: 11 · 2017: 9 · 2016: 9 · 2015: 12 · 2014: 6 · 2013: 6 · 2012: 3
FWCI
1.65
Type
journal article
Language
en
Open access
Licence
Locations
  • Microbiological Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Russian Foundation for Basic Research
  • Russian Academy of Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Marine Sponges and Natural Products
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
Keywords
Arcidae, antimicrobial activity, hemolytic activity, surfactants, cyclic depsipeptides, low molecular weight metabolites, Pseudoalteromonas, Aeromonas, Bacillus pumilus, Paenibacillus, Saccharothrix, Pseudomonas, Sphingomonas, Gammaproteobacteria, Alphaproteobacteria, bioactive metabolites, Sea of Japan
MeSH terms
Animals, Antibiosis, Anti-Bacterial Agents, Gram-Negative Bacteria, Gram-Positive Bacteria, Hemolysis, Japan, Microbial Sensitivity Tests, Molecular Sequence Data, Mollusca, Phylogeny, RNA, Ribosomal, 16S, Seawater, Sequence Analysis, DNA

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review