Current approaches to genetic modification of marine bacteria and considerations for improved transformation efficiency
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Marine bacteria play vital roles in symbiosis, biogeochemical cycles and produce novel bioactive compounds and enzymes of interest for the pharmaceutical, biofuel and biotechnology industries. At present, investigations into marine bacterial functions and their products are primarily based on phenotypic observations, -omic type approaches and heterologous gene expression. To advance our understanding of marine bacteria and harness their full potential for industry application, it is critical that we have the appropriate tools and resources to genetically manipulate them in situ. However, current genetic tools that are largely designed for model organisms such as E. coli, produce low transformation efficiencies or have no transfer ability in marine bacteria. To improve genetic manipulation applications for marine bacteria, we need to improve transformation methods such as conjugation and electroporation in addition to identifying more marine broad host range plasmids. In this review, we aim to outline the reported methods of transformation for marine bacteria and discuss the considerations for each approach in the context of improving efficiency. In addition, we further discuss marine plasmids and future research areas including CRISPR tools and their potential applications for marine bacteria.
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Electroporation employs short, high-voltage electrical pulses to transiently disrupt the cell membrane, facilitating the entry of DNA molecules into the cell.
“This technique employs short, high-voltage electrical pulses to transiently disrupt the cell membrane, facilitating the entry of DNA molecules into the cell [83].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [83]
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Adaptive laboratory evolution and mechanisms of salt tolerance in Lactiplantibacillus plantarum
Han, J., Sun, Z., Chen, Y., Guo, J., Zhang, S., & Ji, C. (2024) · Food Bioscience · doi:10.1016/j.fbio.2024.105811 · Cited by 17 · see citing works
Engineered Marine Biofilms for Ocean Environment Monitoring
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“This technique employs short, high-voltage electrical pulses to transiently disrupt the cell membrane, facilitating the entry of DNA molecules into the cell [83].”
Genetic Changes, Epigenetic Modifications and Antibiotic Resistance Caused by Bacterial Infection
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Marine-based Drug Candidates: Cutting-edge Innovations and Strategies in Modern Drug Discovery
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The development of RPA–CRISPR/Cas12a assay for the detection of Pseudomonas plecoglossicida
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- Authors
- Christi, K., Hudson, J., & Egan, S.
- Affiliations
- UNSW Sydney (AU)
- Corresponding author
- Egan, Suhelen
- Venue
- Microbiological Research
- Publisher
- Elsevier BV
- Published
- 2024-07
- Volume
- 284
- Pages
- 127729
- ISSN
- 0944-5013 (ISSN-L)
- OpenAlex
- W4394988353
- Cited by
- 8
- References
- 189
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 2
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Microbiological Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Gordon and Betty Moore Foundation (9329)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- marine bacteria, genetic modification, transformation efficiency, conjugation, electroporation, broad-host-range plasmids, heterologous expression, bioactive compounds, biotechnology
- MeSH terms
- Bacteria, Conjugation, Genetic, Escherichia coli, Genetic Engineering, Plasmids, Seawater, Transformation, Bacterial, Transformation, Genetic, Electroporation, Aquatic Organisms, CRISPR-Cas Systems
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