Cited by 8Bacterial Genetics and BiotechnologyCRISPR and Genetic EngineeringMicrobial Metabolic Engineering and Bioproduction

Current approaches to genetic modification of marine bacteria and considerations for improved transformation efficiency

Open access
Christi, Katrina, Hudson, Jennifer, Egan, Suhelen·2024·Microbiological Research

Abstract

Marine bacteria play vital roles in symbiosis, biogeochemical cycles and produce novel bioactive compounds and enzymes of interest for the pharmaceutical, biofuel and biotechnology industries. At present, investigations into marine bacterial functions and their products are primarily based on phenotypic observations, -omic type approaches and heterologous gene expression. To advance our understanding of marine bacteria and harness their full potential for industry application, it is critical that we have the appropriate tools and resources to genetically manipulate them in situ. However, current genetic tools that are largely designed for model organisms such as E. coli, produce low transformation efficiencies or have no transfer ability in marine bacteria. To improve genetic manipulation applications for marine bacteria, we need to improve transformation methods such as conjugation and electroporation in addition to identifying more marine broad host range plasmids. In this review, we aim to outline the reported methods of transformation for marine bacteria and discuss the considerations for each approach in the context of improving efficiency. In addition, we further discuss marine plasmids and future research areas including CRISPR tools and their potential applications for marine bacteria.

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Crossref · 10.1016/j.micres.2024.127729

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Authors
Christi, K., Hudson, J., & Egan, S.
Affiliations
UNSW Sydney (AU)
Corresponding author
Egan, Suhelen
Venue
Microbiological Research
Publisher
Elsevier BV
Published
2024-07
Volume
284
Pages
127729
ISSN
0944-5013 (ISSN-L)
OpenAlex
W4394988353
Cited by
8
References
189
Citations by year
2026: 3 · 2025: 3 · 2024: 2
FWCI
0.55
Type
journal article
Language
en
Open access
Locations
  • Microbiological Research (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Gordon and Betty Moore Foundation (9329)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
marine bacteria, genetic modification, transformation efficiency, conjugation, electroporation, broad-host-range plasmids, heterologous expression, bioactive compounds, biotechnology
MeSH terms
Bacteria, Conjugation, Genetic, Escherichia coli, Genetic Engineering, Plasmids, Seawater, Transformation, Bacterial, Transformation, Genetic, Electroporation, Aquatic Organisms, CRISPR-Cas Systems

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