Cited by 78Microbial Metabolic Engineering and BioproductionClostridium difficile and Clostridium perfringens researchBacterial Genetics and Biotechnology

Technical guide for genetic advancement of underdeveloped and intractable Clostridium

Pyne, Michael E., Bruder, Mark, Moo-Young, Murray, Chung, Duane A., Chou, C. Perry·2014·Biotechnology Advances

Abstract

In recent years, the genus Clostridium has risen to the forefront of both medical biotechnology and industrial biotechnology owing to its potential in applications as diverse as anticancer therapy and production of commodity chemicals and biofuels. The prevalence of hyper-virulent strains of C. difficile within medical institutions has also led to a global epidemic that demands a more thorough understanding of clostridial genetics, physiology, and pathogenicity. Unfortunately, Clostridium suffers from a lack of sophisticated genetic tools and techniques which has hindered the biotechnological exploitation of this important bacterial genus. This review provides a comprehensive summary of biotechnological progress made in clostridial genetic tool development, while also aiming to serve as a technical guide for the advancement of underdeveloped clostridial strains, including recalcitrant species, novel environmental samples, and non-type strains. Relevant strain engineering techniques, from genome sequencing and establishment of a gene transfer methodology through to deployment of advanced genome editing procedures, are discussed in detail to provide a blueprint for future clostridial strain construction endeavors. It is expected that a more thorough and rounded-out genetic toolkit available for use in the clostridia will bring about the construction of superior bioprocessing strains and a more complete understanding of clostridial genetics, physiology, and pathogenicity.

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Crossref · 10.1016/j.biotechadv.2014.04.003

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Authors
Pyne, M. E., Bruder, M., Moo-Young, M., Chung, D. A., & Chou, C. P.
Affiliations
University of Waterloo (CA)
Corresponding author
Chung, Duane A.
Venue
Biotechnology Advances
Publisher
Elsevier BV
Published
2014-05
Volume
32
Issue
3
Pages
623-641
ISSN
0734-9750 (ISSN-L)
OpenAlex
W2106986485
Cited by
78
References
198
Citations by year
2025: 4 · 2024: 5 · 2023: 4 · 2022: 2 · 2021: 6 · 2020: 13 · 2019: 14 · 2018: 6 · 2017: 2 · 2016: 10 · 2015: 7 · 2014: 4
FWCI
0.72
Type
journal article
Language
en
Open access
closed
Locations
  • Biotechnology Advances (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Natural Sciences and Engineering Research Council of Canada
  • Canada Research Chairs
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Clostridium, genetic instruments, genome editing, gene transfer, strain engineering, Clostridium difficile, hypervirulent strains, genome sequencing, industrial biotechnology, medical biotechnology
MeSH terms
Clostridium, Genetic Engineering, Industrial Microbiology, Genome, Bacterial, Biofuels

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