Cited by 93Force Microscopy Techniques and ApplicationsDNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniques

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Preparation of DNA and nucleoprotein samples for AFM imaging

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Lyubchenko, Yuri L.·2011·Micron

DOI 10.1016/j.micron.2010.08.011PMC PMC2997872

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Abstract

Sample preparation techniques allowing reliable and reproducible imaging of DNA with various structures, topologies and complexes with proteins are reviewed. The major emphasis is given to methods utilizing chemical functionalization of mica, enabling preparation of the surfaces with required characteristics. The methods are illustrated by examples of imaging of different DNA structures. Special attention is given to the possibility of AFM to image the dynamics of DNA at the nanoscale. The capabilities of time-lapse AFM in aqueous solutions are illustrated by imaging of dynamic processes as transitions of local alternative structures (transition of DNA between H and B forms). The application of AFM to studies of protein-DNA complexes is illustrated by a few examples of imaging site-specific complexes, as well as such systems as chromatin. The time-lapse AFM studies of protein-DNA complexes including very recent advances with the use of high-speed AFM are reviewed.

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Authors: Lyubchenko, Y. L.
Affiliations: Nebraska Medical Center (US)
Corresponding author: Lyubchenko, Yuri L.
ORCID: Lyubchenko, Yuri L.
Venue: Micron
Publisher: Elsevier BV
Published: 2011-02
Volume: 42
Issue: 2
Pages: 196-206
ISSN: 0968-4328 (ISSN-L)
DOI: 10.1016/j.micron.2010.08.011
PMC: PMC2997872
OpenAlex: W2072317160
Cited by: 93
References: 114
Citations by year: 2026: 3 · 2025: 4 · 2024: 4 · 2023: 4 · 2022: 1 · 2021: 8 · 2020: 3 · 2019: 8 · 2018: 11 · 2017: 8 · 2016: 4 · 2015: 4 · 2014: 6 · 2013: 10 · 2012: 7
FWCI: 0.77
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Micron (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Institute of General Medical Sciences (GM62235, R01GM062235)
Topics: Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
Keywords: DNA imaging, nucleoprotein complexes, protein-DNA complex, chromatin, DNA dynamics, high-speed AFM
MeSH terms: DNA, DNA, Superhelical, Nucleic Acid Conformation, Nucleoproteins, Microscopy, Atomic Force

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