The DOI matches its Crossref record; 113 of the 114 references it cites were found in a registry or an index.
Preparation of DNA and nucleoprotein samples for AFM imaging
Open accessAbstract
Sample preparation techniques allowing reliable and reproducible imaging of DNA with various structures, topologies and complexes with proteins are reviewed. The major emphasis is given to methods utilizing chemical functionalization of mica, enabling preparation of the surfaces with required characteristics. The methods are illustrated by examples of imaging of different DNA structures. Special attention is given to the possibility of AFM to image the dynamics of DNA at the nanoscale. The capabilities of time-lapse AFM in aqueous solutions are illustrated by imaging of dynamic processes as transitions of local alternative structures (transition of DNA between H and B forms). The application of AFM to studies of protein-DNA complexes is illustrated by a few examples of imaging site-specific complexes, as well as such systems as chromatin. The time-lapse AFM studies of protein-DNA complexes including very recent advances with the use of high-speed AFM are reviewed.
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Dynamics of Nucleosomes Assessed with Time-Lapse High-Speed Atomic Force Microscopy
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Self-citationMica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy
Shlyakhtenko, L. S., Gall, A. A., & Lyubchenko, Y. L. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-056-4_14 · Cited by 115
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Metadata
- Authors
- Lyubchenko, Y. L.
- Affiliations
- Nebraska Medical Center (US)
- Corresponding author
- Lyubchenko, Yuri L.
- ORCID
- Lyubchenko, Yuri L.
- Venue
- Micron
- Publisher
- Elsevier BV
- Published
- 2011-02
- Volume
- 42
- Issue
- 2
- Pages
- 196-206
- ISSN
- 0968-4328 (ISSN-L)
- PMC
- PMC2997872
- OpenAlex
- W2072317160
- Cited by
- 93
- References
- 114
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 4 · 2022: 1 · 2021: 8 · 2020: 3 · 2019: 8 · 2018: 11 · 2017: 8 · 2016: 4 · 2015: 4 · 2014: 6 · 2013: 10 · 2012: 7
- FWCI
- 0.77
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Micron (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of General Medical Sciences (GM62235, R01GM062235)
- Topics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- DNA imaging, nucleoprotein complexes, protein-DNA complex, chromatin, DNA dynamics, high-speed AFM
- MeSH terms
- DNA, DNA, Superhelical, Nucleic Acid Conformation, Nucleoproteins, Microscopy, Atomic Force
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