In Vitro assessment of the utility of stearyl triphenyl phosphonium modified liposomes in overcoming the resistance of ovarian carcinoma Ovcar-3 cells to paclitaxel
Abstract
Paclitaxel loaded in liposomes modified with stearyl triphenyl phosphonium (STPP) showed improved mitochondrial colocalization and cytotoxicity in a paclitaxel resistant cell line. The improvement in cytotoxicity was not solely due to the increased accumulation of paclitaxel in mitochondria but also due to the specific toxicity of STPP towards the resistant cell line. Mechanistic studies revealed that the cytotoxicity of STPP was associated with a decrease in mitochondrial membrane potential and other hallmarks related to caspase-independent cell death (CICD). This specific toxicity of STPP towards the paclitaxel resistant cell line was also maintained in three-dimensional in vitro spheroid cultures.
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Several chemotherapeutic agents, such as doxorubicin and epirubicin, exhibit inherent fluorescence, enabling direct visualization of their spatial distribution within spheroids using fluorescence or confocal microscopy.
“Several chemotherapeutic agents, such as doxorubicin and epirubicin, exhibit inherent fluorescence, enabling direct visualization of their spatial distribution within spheroids using fluorescence or confocal microscopy [291,292].”
From Modeling Tumor Microenvironment Complexity In Vitro: Spheroids as Physiologically Relevant Tumor Models and Strategies for Their Analysis (2025) · cites this paper as [292]
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- Authors
- Solomon, M. A., Shah, A. A., & D'Souza, G. G. M.
- Affiliations
- Massachusetts College of Pharmacy and Health Sciences (US)
- ORCID
- Solomon, Melani A.
- Venue
- Mitochondrion
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 13
- Issue
- 5
- Pages
- 464-472
- ISSN
- 1567-7249 (ISSN-L)
- OpenAlex
- W2003335129
- Cited by
- 39
- References
- 46
- Citations by year
- 2026: 1 · 2025: 3 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 8 · 2014: 4
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mitochondrion (publishedVersion)
- PubMed (publishedVersion)
- Funders
- MCPHS Faculty Development Committee
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- liposomes, paclitaxel resistance, ovarian carcinoma, OVCAR-3 cells, mitochondrial colocalization, mitochondrial membrane potential, caspase-independent cell death, liposomal drug delivery, three-dimensional spheroid culture, cytotoxicity
- MeSH terms
- Antineoplastic Agents, Cell Survival, Drug Carriers, Female, Humans, Liposomes, Mitochondria, Cell Death, Paclitaxel, Cell Line, Tumor, Membrane Potential, Mitochondrial
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