Alteration of serum/glucocorticoid regulated kinase-1 (sgk-1) gene expression in rat hippocampus after transient global ischemia
Abstract
Expression of the serum/glucocorticoid regulated kinase-1 (sgk-1) gene has been reported to be induced by various stress stimuli such as hyper- or hypo-osmotic stress, UV irradiation, and heat shock stress; however, its association with global ischemia in the brain has not been studied. Using high-density oligonucleotide array analysis, we found that the sgk-1 gene was one of the genes showing alteration of expression in the rat hippocampus during 1-4 h of reperfusion after 10 min of transient global cerebral ischemia. Using TaqMan RT-PCR analysis, we confirmed an increased level of sgk-1 gene expression with statistical significance in the rat hippocampus at 2 h of reperfusion after 10 min of transient global cerebral ischemia. Using in situ hybridization (ISH) analysis, the increased level of sgk-1 gene expression was found to localize in pyramidal cells of CA2 and CA3 regions of the hippocampus after 2 h of reperfusion. These results provide an insight into the alterations of sgk-1 gene expression in the rat hippocampus after transient global cerebral ischemia.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Post-MCAO, SGK1 levels initially double compared to sham-operated rats at 6 hours before falling below control levels after 24 hours, accompanied by an increase in neuronal apoptosis.
“post-MCAO, the levels initially double compared to sham-operated rats at 6 hours before falling below control levels after 24 hours, accompanied by an increase in neuronal apoptosis”
From Serum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain (2024) · cites this paper as Nishida et al., 2004
In rat hippocampal neurons, SGK1 levels significantly drop following OGD and further decline over time.
“In rat hippocampal neurons, SGK1 levels significantly drop following OGD and further decline over time”
From Serum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain (2024) · cites this paper as Nishida et al., 2004
Cited by
Sorted by citations
(Patho)physiological Significance of the Serum- and Glucocorticoid-Inducible Kinase Isoforms
Lang, F., Böhmer, C., Palmada, M., Seebohm, G., Strutz-Seebohm, N., & Vallon, V. (2006) · Physiological Reviews · doi:10.1152/physrev.00050.2005 · Cited by 689 · see citing works
SGK1 Kinase Upregulates GLUT1 Activity and Plasma Membrane Expression
Palmada, M., Boehmer, C., Akel, A., Rajamanickam, J., Jeyaraj, S., Keller, K., & Lang, F. (2006) · Diabetes · doi:10.2337/diabetes.55.02.06.db05-0720 · Cited by 71 · see citing works
Serum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain
Howard, P. G., Zou, P., Zhang, Y., Huang, F., Tesic, V., Wu, C. Y.-C., & Lee, R. H.-C. (2024) · Experimental Neurology · doi:10.1016/j.expneurol.2024.114973 · Cited by 13 · see citing works
Computational insights into the active structure of SGK1 and its implication for ligand design
Akhoon, B. A., Gandhi, N. S., & Pandey, R. (2019) · Biochimie · doi:10.1016/j.biochi.2019.07.007 · Cited by 9 · see citing works
Wang, D., Huang, Z., Li, L., Yuan, Y., Xiang, L., Wu, X., Ni, C., & Yu, W. (2019) · Molecular Medicine Reports · doi:10.3892/mmr.2019.10599 · Cited by 7 · see citing works
- Authors
- Nishida, Y., Nagata, T., Takahashi, Y., Sugahara-Kobayashi, M., Murata, A., & Asai, S.
- Affiliations
- Nihon University (JP)
- Corresponding author
- Asai, Satoshi
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 2004-04
- Volume
- 123
- Issue
- 1-2
- Pages
- 121-125
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W2031762224
- Cited by
- 64
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 1 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 1
- FWCI
- 4.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Nihon University (03-036, 14570777)
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Endoplasmic Reticulum Stress and Disease
- Keywords
- SGK1, gene expression, transient global cerebral ischemia, hippocampus, reperfusion, ischemic brain injury, rats
- MeSH terms
- Serum-Glucocorticoid Regulated Kinases, Animals, Ischemic Attack, Transient, Disease Models, Animal, Glucocorticoids, Glucose, Hippocampus, Male, Nuclear Proteins, Reaction Time, RNA, Messenger, Reperfusion Injury, Up-Regulation, Gene Expression Regulation, Enzymologic, Rats, Wistar, Protein Serine-Threonine Kinases, Immediate-Early Proteins, Pyramidal Cells, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
