Intracarotid cold saline infusion contributes to neuroprotection in MCAO‑induced ischemic stroke in rats via serum and glucocorticoid‑regulated kinase 1
Open accessAbstract
Intracarotid cold saline infusion (ICSI) brings about neuroprotective effects in ischemic stroke. However, the involvement of serum and glucocorticoid‑regulated kinase 1 (SGK1) in the underlying mechanism of ICSI is not fully understood; therefore, we used the rat middle cerebral artery occlusion (MCAO) model to investigate the neuroprotective effects of ICSI on ischemic stroke in rats, as well as the involvement of SGK1 in these effects. ICSI decreased infarct size and brain swelling, as determined by 2,3,5‑triphenyltetrazolium chloride staining and the dry‑wet weight method, respectively. The results of terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL) and Nissl staining showed that ICSI also suppressed apoptosis and increased the relative integral optical density (IOD) values of Nissl bodies in the rat MCAO model. Regarding the mechanism, the results of immunohistochemistry and western blotting revealed that ICSI upregulated SGK1 expression and downregulated beclin‑1 and LC‑3 expression in the rat MCAO model. In addition, SGK1 knockdown increased ICSI‑mediated infarct size and brain swelling, promoted apoptosis, and reduced the IOD values of Nissl bodies in the rat MCAO model. In addition, we found that SGK1 knockdown upregulated beclin‑1 and LC‑3 expression mediated by ICSI. Overall, ICSI had a neuroprotective effect on ischemic stroke after reperfusion by upregulating SGK1 and inhibiting autophagy.
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SGK1-knockdown leads to increased infarct size and brain edema, promotes neuronal apoptosis, and reduces Nissl bodies in rat MCAO model.
“SGK1-knockdow leads to increased infarct size and brain edema, promotes neuronal apoptosis, and reduces Nissl bodies in the rat MCAO model”
From Serum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain (2024) · cites this paper as Wang et al., 2019
The knockdown results sections report larger infarcts, more brain swelling, and increased neuronal apoptosis in cold-saline-treated rats with MCAO. The Nissl-body finding is inconsistent: the results section says relative IOD increased, while the Discussion says it decreased. The experiments also concern rats receiving intracarotid cold saline infusion, a condition omitted from the claim. Page numbers are unavailable in our copy. (LLM, conf=0.86)
Experimental — accuracy not yet measuredAfter MCAO in rats, knockout of SGK1 upregulates expression of beclin-1 and LC-3, aggravating dysregulated autophagy and neuronal apoptosis.
“After MCAO in rats, knockout of SGK1 upregulates the expression of beclin-1 and LC-3, aggravating dysregulated autophagy and neuronal apoptosis”
From Serum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain (2024) · cites this paper as Wang et al., 2019
The Discussion reports that SGK1 knockdown upregulated beclin-1 and LC-3 in the rat MCAO model, and the apoptosis results report increased apoptosis. However, the experiments used shRNA knockdown, not knockout, and tested these effects in rats receiving intracarotid cold saline infusion. The claim omits that treatment condition and describes the autophagy effect more strongly than the measured marker changes establish. (LLM, conf=0.87)
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Self-citation
- Authors
- Wang, D., Huang, Z., Li, L., Yuan, Y., Xiang, L., Wu, X., Ni, C., & Yu, W.
- Affiliations
- Department of Interventional Radiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
- Affiliated Hospital of Guizhou Medical University (CN)
- Guizhou University (CN)
- Guiyang Medical University (CN)
- First Affiliated Hospital of Soochow University (CN)
- Venue
- Molecular Medicine Reports
- Publisher
- Spandidos Publications
- Published
- 2019-08-21
- Volume
- 20
- Issue
- 4
- Pages
- 3942-3950
- ISSN
- 1791-2997 (ISSN-L)
- 1791-3004
- OpenAlex
- W2969508581
- Cited by
- 7
- References
- 45
- Citations by year
- 2024: 3 · 2023: 1 · 2022: 1 · 2021: 2
- FWCI
- 0.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Medicine Reports (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Topics
- Health Sciences › Medicine › Epidemiology › Acute Ischemic Stroke Management
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Thermal Regulation in Medicine
- Keywords
- ischemic stroke, neuroprotection, SGK1, middle cerebral artery occlusion, autophagy, Beclin-1, LC3, apoptosis, infarct size, cerebral edema, ischemia-reperfusion injury
- MeSH terms
- Neuroprotection, Saline Solution, Serum-Glucocorticoid Regulated Kinases, Animals, Brain, Brain Edema, Cold Temperature, Infusions, Intra-Arterial, Male, Up-Regulation, Rats, Sprague-Dawley, Apoptosis, Protein Serine-Threonine Kinases, Immediate-Early Proteins, Neuroprotective Agents, Infarction, Middle Cerebral Artery, RNA Interference, Rats
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