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Molecular Architecture of the Human Pre-mRNA 3′ Processing Complex
Open accessAbstract
SUMMARY Pre-mRNA 3′-end formation is an essential step in eukaryotic gene expression. Over half of human genes produce alternatively polyadenylated mRNAs, suggesting that regulated polyadenylation is an important mechanism for post-transcriptional gene control. Although a number of mammalian mRNA 3′ processing factors have been identified, the full protein composition of the 3′ processing machinery has not been determined, and its structure is unknown. Here we report the purification and subsequent proteomic and structural characterization of human mRNA 3′ processing complexes. Remarkably, the purified 3′ processing complex contains ~85 proteins, including known and new core 3′ processing factors and over 50 proteins that may mediate crosstalk with other processes. Electron microscopic analyses show that the core 3′ processing complex has a distinct “kidney” shape and is ~250 Å in length. Together, our data has revealed the complexity and molecular architecture of the pre-mRNA 3′ processing complex.
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Cites
Mechanism and regulation of mRNA polyadenylation
Colgan, D. F., & Manley, J. L. (1997) · Genes & Development · doi:10.1101/gad.11.21.2755 · Cited by 784
Self-citation
Cited by
Mayr, C., & Bartel, D. P. (2009) · Cell · doi:10.1016/j.cell.2009.06.016 · Cited by 1,613
Alternative polyadenylation of mRNA precursors
Tian, B., & Manley, J. L. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2016.116 · Cited by 1,179
Self-citationMechanisms and Consequences of Alternative Polyadenylation
Di Giammartino, D. C., Nishida, K., & Manley, J. L. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.08.017 · Cited by 737
Self-citationLong Noncoding RNAs with snoRNA Ends
Yin, Q.-F., Yang, L., Zhang, Y., Xiang, J.-F., Wu, Y.-W., Carmichael, G. G., & Chen, L.-L. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.07.033 · Cited by 444
RNA-binding proteins in tumor progression
Qin, H., Ni, H., Liu, Y., Yuan, Y., Xi, T., Li, X., & Zheng, L. (2020) · Journal of Hematology & Oncology · doi:10.1186/s13045-020-00927-w · Cited by 375
Metadata
- Authors
- Shi, Y., Di Giammartino, D. C., Taylor, D., Sarkeshik, A., Rice, W. J., Yates, J. R., Frank, J., & Manley, J. L.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2009-02
- Volume
- 33
- Issue
- 3
- Pages
- 365-376
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2093295511
- Cited by
- 557
- References
- 1
- FWCI
- 14.16
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Shi, Y., Di Giammartino, D. C., Taylor, D., Sarkeshik, A., Rice, W. J., Yates, J. R., Frank, J., & Manley, J. L. (2009). Molecular Architecture of the Human Pre-mRNA 3′ Processing Complex. Molecular Cell. https://doi.org/10.1016/j.molcel.2008.12.028
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