The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Docking of a Specialized PIP Box onto Chromatin-Bound PCNA Creates a Degron for the Ubiquitin Ligase CRL4Cdt2
Open accessAbstract
Summary Substrates of the E3 ubiquitin ligase CRL4Cdt2, including Cdt1 and p21, contain a PCNA-binding motif called a PIP box. Upon binding of the PIP box to PCNA on chromatin, CRL4Cdt2 is recruited and the substrate is ubiquitylated. Importantly, a PIP box cannot be sufficient for destruction, as most PIP box proteins are stable. Using Xenopus egg extracts, we identify two sequence elements in CRL4Cdt2 substrates that promote their proteolysis: a specialized PIP box which confers exceptionally efficient PCNA binding, and a basic amino acid four residues downstream of the PIP box, which recruits CRL4Cdt2 to the substrate-PCNA complex. We also identify two mechanisms which couple CRL4Cdt2-dependent proteolysis to the chromatin-bound form of PCNA, insuring that this proteolysis pathway is active only in S phase or after DNA damage. Thus, CRL4Cdt2 recognizes an unusual degron, which is assembled specifically on chromatin via the binding of a specialized PIP box to PCNA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Function and regulation of cullin–RING ubiquitin ligases
Petroski, M. D., & Deshaies, R. J. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1547 · Cited by 2,148
PCNA, the Maestro of the Replication Fork
Moldovan, G.-L., Pfander, B., & Jentsch, S. (2007) · Cell · doi:10.1016/j.cell.2007.05.003 · Cited by 1,796 · cited 3×
“Many proteins involved in DNA metabolism interact with PCNA, including DNA polymerases, ligases, endonucleases, chromatin remodeling factors, and CDK inhibitors (Moldovan et al., 2007).”
InfluentialThe anaphase promoting complex/cyclosome: a machine designed to destroy
Peters, J.-M. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1988 · Cited by 1,315
Jin, J., Arias, E. E., Chen, J., Harper, J. W., & Walter, J. C. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.08.010 · Cited by 632
Self-citationDCAFs, the Missing Link of the CUL4-DDB1 Ubiquitin Ligase
Lee, J., & Zhou, P. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.06.001 · Cited by 460
The CRL4Cdt2 ubiquitin ligase targets the degradation of p21Cip1 to control replication licensing
Kim, Y., Starostina, N. G., & Kipreos, E. T. (2008) · Genes & Development · doi:10.1101/gad.1703708 · Cited by 240
Nakanishi, M., Robetorye, R. S., Pereira-Smith, O. M., & Smith, J. R. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.29.17060 · Cited by 92
Cited by
Van Roey, K., Uyar, B., Weatheritt, R. J., Dinkel, H., Seiler, M., Budd, A., Gibson, T. J., & Davey, N. E. (2014) · Chemical Reviews · doi:10.1021/cr400585q · Cited by 502
Regulation of PCNA–protein interactions for genome stability
Mailand, N., Gibbs-Seymour, I., & Bekker-Jensen, S. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3562 · Cited by 377
Forging Ahead through Darkness: PCNA, Still the Principal Conductor at the Replication Fork
Choe, K. N., & Moldovan, G.-L. (2017) · Molecular Cell · doi:10.1016/j.molcel.2016.12.020 · Cited by 325
The Many Roles of PCNA in Eukaryotic DNA Replication
(2016) · The Enzymes · doi:10.1016/bs.enz.2016.03.003 · Cited by 294
The histone H4 Lys 20 methyltransferase PR-Set7 regulates replication origins in mammalian cells
Tardat, M., Brustel, J., Kirsh, O., Lefevbre, C., Callanan, M., Sardet, C., & Julien, E. (2010) · Nature Cell Biology · doi:10.1038/ncb2113 · Cited by 289
Metadata
- Authors
- Havens, C. G., & Walter, J. C.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2009-07
- Volume
- 35
- Issue
- 1
- Pages
- 93-104
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W1998631635
- Cited by
- 179
- References
- 7
- FWCI
- 7.44
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Docking of a Specialized PIP Box onto Chromatin-Bound PCNA Creates a Degron for the Ubiquitin Ligase CRL4Cdt2. https://app.cochat.ai/papers/10.1016/j.molcel.2009.05.012
- The paper
- Havens, C. G., & Walter, J. C. (2009). Docking of a Specialized PIP Box onto Chromatin-Bound PCNA Creates a Degron for the Ubiquitin Ligase CRL4Cdt2. Molecular Cell. https://doi.org/10.1016/j.molcel.2009.05.012
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
