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Yeast Pre-rRNA Processing and Modification Occur Cotranscriptionally
Open accessAbstract
To better understand yeast ribosome synthesis, we developed techniques for the rapid harvesting and analysis of metabolically labeled cultures. Modeling of the resulting kinetic data allowed predicted lifetimes and processing patterns to be compared with the experimental data. This supported a transcription time for the 35S primary transcripts of approximately 170 s at 30 degrees C (approximately 40 nt s(-1)), with a high fraction (approximately 70%) of nascent transcripts cleaved at the early processing sites that generate the 20S precursor to the 18S rRNA. This level of nascent transcript cleavage apparently conflicted with previous reports that modification of yeast pre-rRNA exclusively occurred on released transcripts. A second round of high-resolution kinetic labeling showed that 20S pre-rRNA predominately undergoes methylation as nascent transcripts, whereas the 27S precursor to the 25S/5.8S rRNAs was partially methylated on the nascent transcript. The results demonstrate that quantitative analyses of pre-rRNA processing can yield important biological insights.
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Cited by
Sloan, K. E., Warda, A. S., Sharma, S., Entian, K.-D., Lafontaine, D. L. J., & Bohnsack, M. T. (2017) · RNA Biology · doi:10.1080/15476286.2016.1259781 · Cited by 707
Eukaryotic ribosome biogenesis at a glance
Thomson, E., Ferreira-Cerca, S., & Hurt, E. (2013) · Journal of Cell Science · doi:10.1242/jcs.111948 · Cited by 324
Blank, M. F., & Grummt, I. (2017) · Transcription · doi:10.1080/21541264.2016.1276658 · Cited by 111
Nascent Transcript Folding Plays a Major Role in Determining RNA Polymerase Elongation Rates
Turowski, T. W., Petfalski, E., Goddard, B. D., French, S. L., Helwak, A., & Tollervey, D. (2020) · Molecular Cell · doi:10.1016/j.molcel.2020.06.002 · Cited by 75
Self-citationMetabolic turnover and dynamics of modified ribonucleosides by 13C labeling
Gameiro, P. A., Encheva, V., Dos Santos, M. S., MacRae, J. I., & Ule, J. (2021) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2021.101294 · Cited by 10
Metadata
- Authors
- Koš, M., & Tollervey, D.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2010-03
- Volume
- 37
- Issue
- 6
- Pages
- 809-820
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2077926408
- Cited by
- 308
- References
- 0
- FWCI
- 8.62
- Type
- journal article
- Language
- en
- Open access
- hybrid
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