Cited by 728RNA modifications and cancerRNA and protein synthesis mechanismsRNA regulation and disease

Tuning the ribosome: The influence of rRNA modification on eukaryotic ribosome biogenesis and function

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Sloan, Katherine E., Warda, Ahmed S., Sharma, Sunny, Entian, Karl-Dieter, Lafontaine, Denis L. J., Bohnsack, Markus T.·2017·RNA Biology

Abstract

rRNAs are extensively modified during their transcription and subsequent maturation in the nucleolus, nucleus and cytoplasm. RNA modifications, which are installed either by snoRNA-guided or by stand-alone enzymes, generally stabilize the structure of the ribosome. However, they also cluster at functionally important sites of the ribosome, such as the peptidyltransferase center and the decoding site, where they facilitate efficient and accurate protein synthesis. The recent identification of sites of substoichiometric 2'-O-methylation and pseudouridylation has overturned the notion that all rRNA modifications are constitutively present on ribosomes, highlighting nucleotide modifications as an important source of ribosomal heterogeneity. While the mechanisms regulating partial modification and the functions of specialized ribosomes are largely unknown, changes in the rRNA modification pattern have been observed in response to environmental changes, during development, and in disease. This suggests that rRNA modifications may contribute to the translational control of gene expression.

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Crossref · 10.1080/15476286.2016.1259781

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  • The pattern of rRNA modification may change during periods of cellular growth, including modifications catalyzed by DKC1.

    “Recent data suggest that the pattern of rRNA modification may change during periods of cellular growth, including modifications catalyzed by complexes such as dyskerin (DKC1) [190,192].”

    From Ribosome Biogenesis as a Putative Bottleneck to Skeletal Muscle Hypertrophy: Mechanisms, Human Evidence, and Practical Modulators (2026) · cites this paper as [190]

    The ‘Partially modified sites in rRNA’ section reports that some rRNA modifications change in response to environmental conditions. The ‘rRNA modifications in development and disease’ section says dyskerin mutations reduce rRNA pseudouridylation. Neither passage establishes that dyskerin-catalyzed modifications change specifically during periods of cellular growth; no page numbers are available in our copy. (LLM, conf=0.84)

    Experimental — accuracy not yet measured
  • snoRNAs guide site-specific cleavage and chemical modification of the 45S pre-rRNA, contributing to proper ribosomal subunit maturation.

    “As discussed above, snoRNAs guide site-specific cleavage and chemical modification of the 45S pre-rRNA, thereby contributing to proper ribosomal subunit maturation [190].”

    From Ribosome Biogenesis as a Putative Bottleneck to Skeletal Muscle Hypertrophy: Mechanisms, Human Evidence, and Practical Modulators (2026) · cites this paper as [190]

    The Introduction’s ‘Modifications guided by small nucleolar RNAs’ section says snoRNAs direct site-specific chemical modifications of pre-rRNA, and ‘Timing and regulation of rRNA modifications’ places many of these modifications during early ribosome biogenesis. The Introduction also mentions pre-rRNA cleavage during subunit maturation, but our copy does not say that snoRNAs guide site-specific cleavage of the 45S precursor. Page numbers are unavailable in our copy. (LLM, conf=0.85)

    Experimental — accuracy not yet measured
  • The 45S pre-rRNA undergoes more than 200 site-specific modifications, predominantly 2'-O-methylation and pseudouridylation.

    “Before maturation, this precursor transcript is subject to more than 200 site-specific modifications, predominantly 2′-O-methylation and pseudouridylation [190].”

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Research works printed but not linked: 1

  1. [99]Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, et al. Structural snapshots of actively translating human ribosomes. Cell 2015; 161: 845- 57; PMID:25957688; https://doi.org/ 10.1016/j.cell.2015.03.052 25957688 PMC4432480 · DOI

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Authors
Sloan, K. E., Warda, A. S., Sharma, S., Entian, K.-D., Lafontaine, D. L. J., & Bohnsack, M. T.
Affiliations
  • Universitätsmedizin Göttingen (DE)
  • Université Libre de Bruxelles (BE)
  • Goethe University Frankfurt (DE)
Corresponding author
Bohnsack, Markus T.
Venue
RNA Biology
Published
2016-12-18
Volume
14
Issue
9
Pages
1138-1152
ISSN
  • 1547-6286 (ISSN-L)
  • 1555-8584
OpenAlex
W2557904026
Cited by
707
References
155
Citations by year
2026: 63 · 2025: 64 · 2024: 81 · 2023: 101 · 2022: 90 · 2021: 90 · 2020: 96 · 2019: 57 · 2018: 47 · 2017: 15 · 2013: 1
FWCI
3.62
Type
journal article
Language
en
Open access
Locations
  • RNA Biology (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles) (submittedVersion, other-oa)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords
rRNA modification, ribosome biogenesis, 80S ribosome, 2'-O-methylation, pseudouridylation, peptidyl transferase center, decoding site, translational regulation, ribosome structure, nucleolus
MeSH terms
Acetylation, Animals, Disease Susceptibility, Eukaryotic Cells, Humans, Methylation, Ribosomes, RNA, Ribosomal, Structure-Activity Relationship, RNA, Small Nucleolar

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