The Methyltransferase Activity of Clr4Suv39h Triggers RNAi Independently of Histone H3K9 Methylation
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In fission yeast, the pericentromeric dg and dh repeats are transcribed and give rise to small interfering RNAs (siRNAs) by a mechanism that depends on the Clr4(suv39h) histone H3 lysine 9 (H3K9) methyltransferase. Here, we show that Clr4 activity promotes the assembly of a tripartite complex composed of the Clr4-containing CLRC complex and complexes involved in siRNA generation. However, unlike dh siRNAs, dg siRNAs accumulate to near wild-type levels in cells with H3K9 substitutions that cannot be methylated. Thus, Clr4 activity controls siRNA amplification from the different repeat regions by different mechanisms, H3K9 methylation dependent versus independent. Furthermore, artificial tethering of Rik1, a core subunit of the CLRC complex, to a euchromatic RNA mediates RNAi-dependent silencing that partially bypasses the requirement for other CLRC subunits. These findings establish Rik1 as a key link between CLRC and RNAi and reveal distinct centromeric siRNA amplification mechanisms that depend on the Clr4 methyltransferase activity.
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Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast
Taglini, F., Chapman, E., van Nues, R. W., Théron, E., & Bayne, E. H. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz1157 · Cited by 13 · see citing works
Ago1 Affects the Virulence of the Fungal Plant Pathogen Zymoseptoria tritici
Habig, M., Schotanus, K., Hufnagel, K., Happel, P., & Stukenbrock, E. H. (2021) · Genes · doi:10.3390/genes12071011 · Cited by 12 · see citing works
Assembly and Functions of Heterochromatin in the Fission Yeast GenomePreprint
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Clr4 specificity and catalytic activity beyond H3K9 methylation
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Heterochromatin and Euchromatin-Organization, Boundaries, and Gene Regulation
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- Authors
- Gerace, E. L., Halic, M., & Moazed, D.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Moazed, Danesh
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2010-08
- Volume
- 39
- Issue
- 3
- Pages
- 360-372
- ISSN
- 1097-2765 (ISSN-L)
- PMC
- PMC2941148
- OpenAlex
- W1999534739
- Cited by
- 70
- References
- 50
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 6 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 5 · 2018: 3 · 2017: 6 · 2016: 6 · 2015: 5 · 2014: 7 · 2013: 2 · 2012: 6
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM061641, GM72805, R01GM072805)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- Clr4, Suv39h, histone H3 lysine 9 methylation, RNA interference, siRNA, pericentromeric repeats, Schizosaccharomyces pombe
- MeSH terms
- Centromere, Chromosomal Proteins, Non-Histone, Histones, Methylation, Methyltransferases, Multienzyme Complexes, Histone-Lysine N-Methyltransferase, RNA, Fungal, Schizosaccharomyces, Cell Cycle Proteins, Gene Silencing, Schizosaccharomyces pombe Proteins, RNA, Small Interfering
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