CRISPR-Based Technologies: Impact of RNA-Targeting Systems
Open accessAbstract
DNA-targeting CRISPR-Cas systems, such as those employing the RNA-guided Cas9 or Cas12 endonucleases, have revolutionized our ability to predictably edit genomes and control gene expression. Here, I summarize information on RNA-targeting CRISPR-Cas systems and describe recent advances in converting them into powerful and programmable RNA-binding and cleavage tools with a wide range of novel and important biotechnological and biomedical applications.
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CRISPR-Cas9 and related enzymes have been used to manipulate the genomes of cultured cells, animals and plants, accelerating fundamental research and enabling breakthroughs in agriculture and synthetic biology.
“CRISPR-Cas9 and related enzymes have been used to manipulate the genomes of cultured cells, animals and plants, vastly accelerating the pace of fundamental research and enabling breakthroughs in agriculture and synthetic biology (reviewed in refs. 6-9).”
From The promise and challenge of therapeutic genome editing (2020) · cites this paper as [9]
The Introduction section on DNA-targeting CRISPR tools (page unavailable) says Cas9 and Cas12 systems are useful across a wide range of organisms and cell types, enable genome editing, and have a major impact on life sciences and applications in agriculture and biotechnology. It does not specifically establish breakthroughs in synthetic biology or identify genome manipulation in both animals and plants, so those details go beyond what this copy states. (LLM, conf=0.82)
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The promise and challenge of therapeutic genome editing
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- Authors
- Terns, M. P.
- Affiliations
- University of Georgia (US)
- Corresponding author
- Terns, Michael P.
- ORCID
- Terns, Michael P.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2018-11
- Volume
- 72
- Issue
- 3
- Pages
- 404-412
- ISSN
- 1097-2765 (ISSN-L)
- PMC
- PMC6239212
- OpenAlex
- W2899456281
- Cited by
- 168
- References
- 79
- Citations by year
- 2026: 11 · 2025: 12 · 2024: 19 · 2023: 21 · 2022: 28 · 2021: 29 · 2020: 30 · 2019: 16 · 2018: 1
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH (R35GM118160)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- CRISPR-Cas, RNA-targeting CRISPR, RNA-guided endonuclease, Cas9, Cas12, RNA cleavage, RNA-binding proteins, genome editing, gene expression regulation, programmable nucleases
- MeSH terms
- Gene Editing, RNA, Guide, CRISPR-Cas Systems, Animals, DNA, Genetic Engineering, Humans, RNA, RNA, Guide, Kinetoplastida, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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