RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex
Open accessAbstract
Compelling evidence indicates that the CRISPR-Cas system protects prokaryotes from viruses and other potential genome invaders. This adaptive prokaryotic immune system arises from the clustered regularly interspaced short palindromic repeats (CRISPRs) found in prokaryotic genomes, which harbor short invader-derived sequences, and the CRISPR-associated (Cas) protein-coding genes. Here, we have identified a CRISPR-Cas effector complex that is comprised of small invader-targeting RNAs from the CRISPR loci (termed prokaryotic silencing (psi)RNAs) and the RAMP module (or Cmr) Cas proteins. The psiRNA-Cmr protein complexes cleave complementary target RNAs at a fixed distance from the 3' end of the integral psiRNAs. In Pyrococcus furiosus, psiRNAs occur in two size forms that share a common 5' sequence tag but have distinct 3' ends that direct cleavage of a given target RNA at two distinct sites. Our results indicate that prokaryotes possess a unique RNA silencing system that functions by homology-dependent cleavage of invader RNAs.
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CRISPR technology is based on a prokaryotic antiviral defense system with CRISPR DNA elements and Cas proteins; CRISPRs derive from bacteriophage genomes and generate RNAs that function with Cas proteins to cleave bacteriophage genomes upon infection.
“CRISPR technology is based on a prokaryotic antiviral defense system characterized by DNA elements termed CRISPRs (clustered regularly interspaced short palindromic repeats) and CRISPR-associated (Cas) proteins. CRISPRs are derived from bacteriophage genomes and generate RNAs that function with Cas proteins to cleave bacteriophage genomes upon infection32.”
From CRISPR-based functional genomics for neurological disease (2020) · cites this paper as [32]
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- Authors
- Hale, C. R., Zhao, P., Olson, S., Duff, M. O., Graveley, B. R., Wells, L., Terns, R. M., & Terns, M. P.
- Affiliations
- University of Georgia (US)
- University of Connecticut (US)
- Corresponding author
- Terns, Rebecca M.
- Terns, Michael P.
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2009-11
- Volume
- 139
- Issue
- 5
- Pages
- 945-956
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC2951265
- OpenAlex
- W2153537688
- Cited by
- 1,031
- References
- 38
- Citations by year
- 2026: 20 · 2025: 34 · 2024: 55 · 2023: 38 · 2022: 71 · 2021: 72 · 2020: 60 · 2019: 68 · 2018: 53 · 2017: 69 · 2016: 78 · 2015: 74 · 2014: 83 · 2013: 93 · 2012: 68
- FWCI
- 21.76
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- OpenCommons at University of Connecticut (University of Connecticut) (submittedVersion)
- OpenCommons - UConn (University of Connecticut) (submittedVersion)
- Funders
- Georgia Cancer Coalition
- University of Georgia
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM54682, R01GM054682, R01GM062516)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- CRISPR-Cas, RNA-guided RNA cleavage, Pyrococcus furiosus, RNA silencing
- MeSH terms
- Base Sequence, RNA, Viral, RNA, Archaeal, Archaeal Proteins, Pyrococcus furiosus, RNA Interference, RNA, Small Untranslated
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