The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.
Mediator Condensates Localize Signaling Factors to Key Cell Identity Genes
Open accessAbstract
SUMMARY The gene expression programs that define each cell’s identity are controlled by master transcription factors (TFs) that bind cell-type specific enhancers, as well as signaling factors, which bring extracellular stimuli to these enhancers. Recent studies have revealed that master TFs form phase-separated condensates with the Mediator coactivator at super-enhancers. Here we present evidence that signaling factors for the WNT, TGF-β and JAK/STAT pathways employ their intrinsically disordered regions (IDRs) to enter and concentrate in Mediator condensates at super-enhancers. We show that the WNT coactivator β-catenin interacts both with components of condensates and DNA binding factors to selectively occupy super-enhancer associated genes. We propose that the cell-type specificity of the response to signaling is mediated, in part, by the IDRs of the signaling factors, which cause these factors to partition into condensates established by the master TFs and Mediator at genes with prominent roles in cell identity.
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“3D) Representative images of in vitro droplet assays of signaling factors with purified Mediator showing the ability of β-catenin, STAT3 and SMAD3 to interact and partition into intact Mediator droplets.”
BackgroundInfluentialMutations affecting segment number and polarity in Drosophila
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RNA-Mediated Feedback Control of Transcriptional Condensates
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Metadata
- Authors
- Zamudio, A. V., Dall’Agnese, A., Henninger, J. E., Manteiga, J. C., Afeyan, L. K., Hannett, N. M., Coffey, E. L., Li, C. H., Oksuz, O., Sabari, B. R., Boija, A., Klein, I. A., Hawken, S. W., Spille, J.-H., Decker, T.-M., Cisse, I. I., Abraham, B. J., Lee, T. I., Taatjes, D. J., Schuijers, J., & Young, R. A.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2019-12
- Volume
- 76
- Issue
- 5
- Pages
- 753-766.e6
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2976392421
- Cited by
- 283
- References
- 66
- FWCI
- 8.38
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Zamudio, A. V., Dall’Agnese, A., Henninger, J. E., Manteiga, J. C., Afeyan, L. K., Hannett, N. M., Coffey, E. L., Li, C. H., Oksuz, O., Sabari, B. R., Boija, A., Klein, I. A., Hawken, S. W., Spille, J.-H., Decker, T.-M., Cisse, I. I., Abraham, B. J., Lee, T. I., Taatjes, D. J., . . . Young, R. A. (2019). Mediator Condensates Localize Signaling Factors to Key Cell Identity Genes. Molecular Cell. https://doi.org/10.1016/j.molcel.2019.08.016
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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