The DOI matches its Crossref record; 81 of the 81 references it cites were found in a registry or an index.
Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems
Open accessAbstract
User-friendly tools for robust transcriptional activation of endogenous genes are highly demanded in plants. We previously showed that a dCas9-VP64 system consisting of the deactivated CRISPR-associated protein 9 (dCas9) fused with four tandem repeats of the transcriptional activator VP16 (VP64) could be used for transcriptional activation of endogenous genes in plants. In this study, we developed a second generation of vector systems for enhanced transcriptional activation in plants. We tested multiple strategies for dCas9-based transcriptional activation, and found that simultaneous recruitment of VP64 by dCas9 and a modified guide RNA scaffold gRNA2.0 (designated CRISPR-Act2.0) yielded stronger transcriptional activation than the dCas9-VP64 system. Moreover, we developed a multiplex transcription activator-like effector activation (mTALE-Act) system for simultaneous activation of up to four genes in plants. Our results suggest that mTALE-Act is even more effective than CRISPR-Act2.0 in most cases tested. In addition, we explored tissue-specific gene activation using positive feedback loops. Interestingly, our study revealed that certain endogenous genes are more amenable than others to transcriptional activation, and tightly regulated genes may cause target gene silencing when perturbed by activation probes. Hence, these new tools could be used to investigate gene regulatory networks and their control mechanisms. Assembly of multiplex CRISPR-Act2.0 and mTALE-Act systems are both based on streamlined and PCR-independent Golden Gate and Gateway cloning strategies, which will facilitate transcriptional activation applications in both dicots and monocots.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In rice, simultaneous multigene activation is possible using the CRISPR activation (CRISPRa) system (Lowder et al., 2018).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Lowder et al., 2018
Citations
Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana
Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 22,014
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Generation of germline-competent induced pluripotent stem cells
Okita, K., Ichisaka, T., & Yamanaka, S. (2007) · Nature · doi:10.1038/nature05934 · Cited by 4,438
Contrasting Mechanisms of Defense Against Biotrophic and Necrotrophic Pathogens
Glazebrook, J. (2005) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.43.040204.135923 · Cited by 4,380
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
Napoli, C. A., Lemieux, C., & Jorgensen, R. A. (1990) · The Plant Cell · doi:10.1105/tpc.2.4.279 · Cited by 2,892
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Konermann, S., Brigham, M. D., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., et al. (2015) · Nature · doi:10.1038/nature14136 · Cited by 2,825
Flagged first, then by citations
Cited by
Sorted by citations
Items 101–200 of 232
Advances in CRISPR/Cas9 genome editing for crop improvement and global food security
Haider, S., Singh, A. P., Panthi, B., Sindhu, S. R., Safa, N. T., Malik, S., & Rahimi, M. (2026) · Current Plant Biology · doi:10.1016/j.cpb.2026.100593 · Cited by 25
Manipulating epigenetic diversity in crop plants: Techniques, challenges and opportunities
Singh, V. K., Ahmed, S. S., Saini, D. K., Gahlaut, V., Chauhan, S., Khandare, K., Kumar, A., Sharma, P. K., & Kumar, J. (2023) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2023.130544 · Cited by 22
Genetic Engineering Technologies for Improving Crop Yield and Quality
Ye, R., Yang, X., & Rao, Y. (2022) · Agronomy · doi:10.3390/agronomy12040759 · Cited by 21
Engineering Synthetic Signaling in Plants
Leydon, A. R., Gala, H., Guiziou, S., & Nemhauser, J. L. (2020) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-081519-035852 · Cited by 21
CRISPR towards a Sustainable Agriculture
Camerlengo, F., Frittelli, A., & Pagliarello, R. (2022) · Encyclopedia · doi:10.3390/encyclopedia2010036 · Cited by 24
Karunarathne, S., Walker, E., Sharma, D., Li, C., & Han, Y. (2023) · Journal of Zhejiang University-SCIENCE B · doi:10.1631/jzus.b2200552 · Cited by 18
“In rice, simultaneous multigene activation is possi‐ ble using the CRISPR activation (CRISPRa) system (Lowder et al., 2018).”
Zheng, X., Qi, C., Yang, L., Quan, Q., Liu, B., Zhong, Z., Tang, X. D., Fan, T., Zhou, J., & Zhang, Y. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00621 · Cited by 22
Self-citationSophisticated CRISPR/Cas tools for fine-tuning plant performance
Capdeville, N., Merker, L., Schindele, P., & Puchta, H. (2020) · Journal of Plant Physiology · doi:10.1016/j.jplph.2020.153332 · Cited by 21
Dissecting Plant Gene Functions Using CRISPR Toolsets for Crop Improvement
Zhang, R., Li, B., Yang, Z.-G., Huang, J., Sun, W.-H., Bhanbhro, N., Liu, W., & Chen, K. (2022) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.2c01754 · Cited by 20
Optimized dCas9 programmable transcriptional activators for plants
Zinselmeier, M. H., Casas-Mollano, J. A., Cors, J., Sychla, A., Heinsch, S. C., Voytas, D. F., & Smanski, M. J. (2024) · Plant Biotechnology Journal · doi:10.1111/pbi.14441 · Cited by 19
Self-citationData Mining by Pluralistic Approach on CRISPR Gene Editing in Plants
Kaul, T., Raman, N. M., Eswaran, M., Thangaraj, A., Verma, R., Sony, S. K., Sathelly, K., Kaul, R., Yadava, P., & Agrawal, P. K. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00801 · Cited by 18
Chromatin Manipulation and Editing: Challenges, New Technologies and Their Use in Plants
Fal, K., Tomková, D., Vachon, G., Chabouté, M., Berr, A., & Carles, C. C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22020512 · Cited by 19
CRISPR/cas-mediated genome editing in sorghum — recent progress, challenges and prospects
Parikh, A., Brant, E. J., Baloglu, M. C., & Altpeter, F. (2021) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-021-10215-y · Cited by 18
Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications
Clauer, P. R., Nou, A., Toth, T., Yu, Q., Chemla, Y., Boo, A., Yoon, K. S., & Voigt, C. A. (2026) · Chemical Reviews · doi:10.1021/acs.chemrev.4c00687 · Cited by 21
Bhambhani, S., Kondhare, K. R., & Giri, A. P. (2021) · Phytochemistry Reviews · doi:10.1007/s11101-021-09749-1 · Cited by 18
Systems biology of root development in Populus: Review and perspectives
Ahkami, A. H. (2023) · Plant Science · doi:10.1016/j.plantsci.2023.111818 · Cited by 22
Targeted genome editing for cotton improvement: prospects and challenges
Kumar, R., Das, J., Raghavendra, K. P., Kumar, M., Balasubramani, G., & Prasad, Y. G. (2024) · The Nucleus · doi:10.1007/s13237-024-00479-1 · Cited by 24
The current progress of CRISPR/Cas9 development in plants
Bhalothia, P., Yajnik, K. N., Alok, A., & Upadhyay, S. K. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818140-9.00011-8 · Cited by 17
Ghose, A. K., Abdullah, S. N. A., Hatta, M. A. M., & Wahab, P. E. M. (2022) · Plants · doi:10.3390/plants11182393 · Cited by 18
The era of editing plant genomes using CRISPR/Cas: A critical appraisal
Bhat, M. A., Bhat, M. A., Kumar, V., Wani, I. A., Bashir, H., Shah, A. A., Rahman, S., & Jan, A. T. (2020) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2020.09.013 · Cited by 17
European genome editing regulations: threats to the European bioeconomy and unfit for purpose
Hjort, C., Cole, J. A., & Frébort, I. (2021) · EFB Bioeconomy Journal · doi:10.1016/j.bioeco.2021.100001 · Cited by 16
Rehman, R. S., Zafar, S. A., Ali, M., Pasha, A. N., Naveed, M. S., Waseem, M., Ahmad, M., & Raza, A. (2022) · Asian Plant Research Journal · doi:10.9734/aprj/2022/v9i130197 · Cited by 16
Versatile and multifaceted CRISPR/Cas gene editing tool for plant research
Pandey, P. K., Quilichini, T. D., Vaid, N., Gao, P., Xiang, D., & Datla, R. (2019) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2019.04.012 · Cited by 18
CRISPR/Cas9 for Heat Stress Tolerance in Rice: A Review
Chakraborty, A., & Wylie, S. J. (2025) · Plant Molecular Biology Reporter · doi:10.1007/s11105-025-01564-8 · Cited by 19
Liu, L., Lindsay, P. L., & Jackson, D. P. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22105167 · Cited by 15
A nuclease-dead Cas9-derived tool represses target gene expression
Wang, B., Liu, X., Li, Z., Zeng, K., Guo, J., Xin, T., Zhang, Z., Li, J., & Yang, X. Y. (2024) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiae149 · Cited by 15
Engineering biotic stress tolerance via CRISPR-Cas mediated genome editing in crop plants
Hussain, A., Munir, M., Khalid, A., Ali, M., Amanullah, M., Ali, Q., & Manghwar, H. (2024) · Plant Stress · doi:10.1016/j.stress.2024.100650 · Cited by 15
Belykh, E. S., Maystrenko, T. A., & Velegzhaninov, I. O. (2019) · Molecular Biotechnology · doi:10.1007/s12033-019-00202-5 · Cited by 15
Genome editing in grass plants
Char, S. N., & Yang, B. (2019) · aBIOTECH · doi:10.1007/s42994-019-00005-x · Cited by 15
From Evolution to Revolution: Accelerating Crop Domestication through Genome Editing
Kumar, K., Mandal, S. N., Pradhan, B., Kaur, P., Kaur, K., & Neelam, K. (2022) · Plant and Cell Physiology · doi:10.1093/pcp/pcac124 · Cited by 15
Development and Application of CRISPR/Cas System in Rice
Ren, J., Hu, X., Kejian, W., & Wang, C. (2019) · Rice Science · doi:10.1016/j.rsci.2019.01.001 · Cited by 17
Design principles for synthetic control systems to engineer plants
Bull, T., & Khakhar, A. (2023) · Plant Cell Reports · doi:10.1007/s00299-023-03072-z · Cited by 14
CRISPR/Cas in Grapevine Genome Editing: The Best Is Yet to Come
Ren, C., Mohamed, M. S. M., Aini, N., Kuang, Y., & Liang, Z. (2024) · Horticulturae · doi:10.3390/horticulturae10090965 · Cited by 18
Advances in plant synthetic biology approaches to control expression of gene circuits
Koukara, J., & Papadopoulou, K. Κ. (2023) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2023.02.061 · Cited by 13
Khumsupan, P., Donovan, S., & McCormick, A. J. (2019) · Physiologia Plantarum · doi:10.1111/ppl.12937 · Cited by 14
Wang, X., Tu, M., Li, Z., Wang, Y., & Wang, X. (2018) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2018.1517457 · Cited by 13
Розов, С. М., Permyakova, N. V., Sidorchuk, Y. V., & Дейнеко, Е. В. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23084416 · Cited by 12
CRISPR-enabled genetic logic circuits for biosensing
Wang, X., Gao, Y., Zhou, N., Yang, Z., Cooper, J. M., & Wang, B. (2025) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2025.118297 · Cited by 13
Expanding horizons of CRISPR applications beyond genome editing
Liang, Y., Tong, S., Zhang, J., Tan, G., Zhang, L., Lee, S. Y., & Tong, Y. (2025) · Trends in Genetics · doi:10.1016/j.tig.2025.06.003 · Cited by 12
Selma, S., Gianoglio, S., Uranga, M., Vázquez‐Vilar, M., Espinosa‐Ruíz, A., Drapal, M., Fraser, P. D., Daròs, J., & Orzáez, D. (2022) · The Plant Journal · doi:10.1111/tpj.15906 · Cited by 14
Salman, M., Verma, A., Singh, V. K., Jaffet, J., Chaurasia, S., Sahel, D. K., Ramappa, M., & Singh, V. (2022) · Asia-Pacific Journal of Ophthalmology · doi:10.1097/apo.0000000000000443 · Cited by 13
Li, Z., Wang, F., & Li, J. (2019) · Current Protocols in Molecular Biology · doi:10.1002/cpmb.89 · Cited by 12
CRISPR-Cas Technology: A Genome-Editing Powerhouse for Molecular Plant Breeding
Mohan, C., Satish, L., Muthubharathi, B. C., Selvarajan, D., Easterling, M., & Yau, Y. (2022) · Springer eBooks · doi:10.1007/978-981-16-9001-3_32 · Cited by 13
Multigene engineering in plants: Technologies, applications, and future prospects
Rajput, R., Boone, B. A., Mandlik, R., Islam, M. T., Qi, Y., Wang, J., Barrangou, R., Sozzani, R., Eckert, C. A., Tuskan, G. A., et al. (2025) · Biotechnology Advances · doi:10.1016/j.biotechadv.2025.108697 · Cited by 11
Self-citationMolecular and Computational Strategies to Increase the Efficiency of CRISPR-Based Techniques
Mattiello, L., Rütgers, M., Sua-Rojas, M. F., Tavares, R., Soares, J. S., Begcy, K., & Menossi, M. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.868027 · Cited by 12
Engineering Tobacco for Plant Natural Product Production
Stephenson, M. J., Reed, J., Patron, N. J., Lomonossoff, G. P., & Osbourn, A. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-409547-2.14724-9 · Cited by 13
Alves, N. S. F., Pinto, C. E. M., Arraes, F. B. M., Costa, L. S. D. L., de Abreu, R. A., Moreira, V. J. V., Lourenço‐Tessutti, I. T., Pinheiro, D. H., Lisei-de-Sa, M. E., de Melo, B. P., et al. (2023) · Planta · doi:10.1007/s00425-023-04286-x · Cited by 10
Contiliani, D. F., Sretenovic, S., Dailey, M., Zhou, M., Cheng, Y., Creste, S., Xiao, S., & Qi, Y. (2025) · Plant Biotechnology Journal · doi:10.1111/pbi.70000 · Cited by 10
Self-citationSelma, S., Ceulemans, E., Goossens, A., & Lacchini, E. (2022) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2022.102856 · Cited by 10
Bioengineering of Canopy Photosynthesis in Rice for Securing Global Food Security: A Critical Review
Vishwakarma, C., Krishna, G. K., Kapoor, R. T., Mathur, K., Lal, S. K., Saini, R. P., Yadava, P., & Chinnusamy, V. (2023) · Agronomy · doi:10.3390/agronomy13020489 · Cited by 15
Conditional and tissue-specific approaches to dissect essential mechanisms in plant development
Pfeiffer, M. L., Winkler, J., Damme, D. V., Jacobs, T. B., & Nowack, M. K. (2021) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2021.102119 · Cited by 10
de Melo, B. P., de Moura, S. M., Morgante, C. V., Pinheiro, D. H., Alves, N. S. F., Rodrigues-Silva, P. L., Lourenço‐Tessutti, I. T., de Andrade, R. V., Fragoso, R. D. R., & Grossi‐de‐Sá, M. F. (2021) · Biotechnology Research and Innovation · doi:10.4322/biori.202105 · Cited by 9
Genome Editing and Designer Crops for the Future
Rana, S., Aggarwal, P., Shukla, V., Giri, U., Verma, S., & Muthamilarasan, M. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-1875-2_3 · Cited by 9
Furci, L., Berthelier, J., Juez, O., Miryeganeh, M., & Saze, H. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-91909-8.00007-4 · Cited by 8
CRISPR/Cas9-mediated genome editing in medicinal and aromatic plants: developments and applications
Shabir, P. A. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-819590-1.00009-4 · Cited by 9
Menelih, A., Girma, A., & Habtemariam, A. A. (2025) · Functional & Integrative Genomics · doi:10.1007/s10142-025-01762-3 · Cited by 8
A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants
Cheng, Y., Li, G., Qi, A., Mandlik, R., Pan, C., Wang, D., Ge, S., & Qi, Y. (2025) · The Plant Cell · doi:10.1093/plcell/koaf081 · Cited by 9
Self-citationMultigene editing: current approaches and beyond
Peng, H., Zheng, Y., Zhao, Z., & Li, J. (2020) · Briefings in Bioinformatics · doi:10.1093/bib/bbaa396 · Cited by 8
CRISPR/Cas13: A Novel and Emerging Tool for RNA Editing in Plants
Pandita, D., Puli, C. O. R., & Palakolanu, S. R. (2021) · Concepts and strategies in plant sciences · doi:10.1007/978-3-030-64994-4_14 · Cited by 8
Available Toolkits for CRISPR/CAS Genome Editing in Plants
Mikhaylova, E. V., Khusnutdinov, E., Chemeris, A. V., & Кулуев, Б. Р. (2022) · Russian Journal of Plant Physiology · doi:10.1134/s1021443722010137 · Cited by 8
CRISPR‐Act3.0‐Based Highly Efficient Multiplexed Gene Activation in Plants
Pan, C., & Qi, Y. (2022) · Current Protocols · doi:10.1002/cpz1.365 · Cited by 8
Self-citationComprehensive benchmarking of genome editing quantification methods for plant applications
Gong, Z., Zhang, Y., Xia, D., Yoon, S., Crisp, P. A., & Botella, J. R. (2025) · iScience · doi:10.1016/j.isci.2025.112350 · Cited by 7
Self-citationCRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation
Malzahn, A. A., Zhang, Y., & Qi, Y. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-8991-1_7 · Cited by 7
Self-citationToward a logic-based framework for plant epigenetic control
Zhang, L., & Zhu, J. (2025) · Trends in Plant Science · doi:10.1016/j.tplants.2025.10.019 · Cited by 7
New and novel genetic tools for improving crops
Suprasanna, P., Saddhe, A. A., Ghuge, S. A., & Ingle, K. P. (2021) · CABI Reviews · doi:10.1079/pavsnnr202116028 · Cited by 7
Genomic-Assisted Breeding Tools for Grain and Nutritional Quality Improvement in Rice
Patra, B., Majhi, P. K., Tripathy, S. K., Tripathy, S. P., Khan, A., Behera, P. P., Das, S., & Ahamad, A. (2022) · International Journal of Environment and Climate Change · doi:10.9734/ijecc/2022/v12i130609 · Cited by 6
Improvement of floricultural traits in ornamental crops using genome editing tools
Jadhav, P. R., Aglawe, S. B., Harish, D. K., Wagh, Y. S., Barbadikar, K. M., Kumar, P. N., Kawar, P. G., Prasad, K. V., & Kumar, S. P. J. (2023) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-023-00851-3 · Cited by 7
From qualitative to quantitative: the state of the art and challenges for plant synthetic biology
Tian, C., Li, J., & Wang, Y. (2023) · Quantitative Biology · doi:10.15302/j-qb-022-0326 · Cited by 5
Plant Functional Genomics Based on High‐Throughput CRISPR Library Knockout Screening: A Perspective
He, J., Zeng, C., & Li, M. (2023) · Advanced Genetics · doi:10.1002/ggn2.202300203 · Cited by 6
Houbaert, A., Tendon, V. D., Hoermayer, L., Morffy, N., Strader, L. C., & Geldner, N. (2025) · Nature Communications · doi:10.1038/s41467-025-61742-w · Cited by 5
Pandey, H., Yadav, B., Shah, K., Kaur, R., Choudhary, D., Sharma, N., & Rishi, V. (2024) · Protein Expression and Purification · doi:10.1016/j.pep.2024.106500 · Cited by 5
Tan, W. Z., Wang, Z., & Liu, L. (2024) · Agriculture · doi:10.3390/agriculture15010029 · Cited by 5
Prasad, M. A., & Shetty, R. (2026) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2026.195139 · Cited by 6
Phytoremediation using CRISPR-Cas9 technology
Sharma, P., Singh, S. P., & Tong, Y. W. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-99907-6.00009-8 · Cited by 5
The Role of Transcription Factors in Response to Biotic Stresses in Maize
Sahoo, J. P., Dash, D., Moharana, A., Mahapatra, M., Sahoo, A. K., & Samal, K. C. (2022) · Springer eBooks · doi:10.1007/978-3-031-12990-2_9 · Cited by 4
CRISPR-Cas epigenome editing: improving crop resistance to pathogens
López-Calleja, A. C., Vizuet‐de‐Rueda, J. C., & Álvarez-Venegas, R. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-821910-2.00030-8 · Cited by 4
Regulatory trends in engineering bioactive-phytocompounds
Saifi, M., Ashrafi, K., Qamar, F., & Abdin, M. Z. (2024) · Plant Science · doi:10.1016/j.plantsci.2024.112167 · Cited by 4
CRISPR Applications in Plant Genetic Engineering and Biotechnology
Srivastava, V. K. (2019) · Springer eBooks · doi:10.1007/978-981-13-8499-8_19 · Cited by 4
Genomic Interventions for Improving Crop Yield and Resilience
Barmukh, R., Thakur, N. R., & Shah, P. (2024) · Advances in plant breeding strategies · doi:10.1007/978-3-031-68586-6_3 · Cited by 3
CRISPR-Cas9: chronology and evolution
Mochi, J. A., Jani, J., Joshi, S. M., & Pappachan, A. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-91808-4.00005-5 · Cited by 3
Targeted Genome Engineering and Its Application in Trait Improvement of Crop Plants
Yang, X., Luo, L., Mo, B., & Liu, L. (2019) · Agricultural Sciences · doi:10.4236/as.2019.1010097 · Cited by 3
Zhu, T., Tang, W., Chen, D., Li, J., & Su, J. (2021) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2020.12.001 · Cited by 3
If Mendel Was Using CRISPR: Genome Editing Meets Non‐Mendelian Inheritance
Hudzieczek, V., Hobza, R., Cápal, P., Šafář, J., & Doležel, J. (2022) · Advanced Functional Materials · doi:10.1002/adfm.202202585 · Cited by 3
Tapping into the Unsung Potential of CRISPR/CAS Technology in Agriculture
Rehman, R. S., Ali, M., Zafar, S. A., Ahmad, M., Pasha, A. N., Bashir, H., Rashid, F., & Hussain, M. (2022) · Asian Journal of Biochemistry Genetics and Molecular Biology · doi:10.9734/ajbgmb/2022/v10i430249 · Cited by 3
Harnessing CRISPR/Cas Tools for Installing Virus Resistance in Cereals: An Overview
Talakayala, A., Ankanagari, S., & Mallikarjuna, G. B. (2022) · Springer eBooks · doi:10.1007/978-981-19-1445-4_13 · Cited by 3
Advancing plant gene activation with CRISPR-Act3.0
Movahedi, A., Mu, Z., & Yang, L. (2025) · Trends in biotechnology · doi:10.1016/j.tibtech.2025.01.008 · Cited by 2
Sun, X., Wu, F., Ma, Z., Liang, G., Chen, S., Hu, X., Fan, S., & Zhao, Y. (2026) · BMC Plant Biology · doi:10.1186/s12870-026-08295-2 · Cited by 2
Dzięgielewski, W., Szymańska-Lejman, M., Wilhelm, A., Hus, K., & Ziółkowski, P. A. (2026) · Science Advances · doi:10.1126/sciadv.aeb2890 · Cited by 3
Crops exposed to extreme conditions: perspectives of gene editing to improve stress tolerance
Szabados, L., Kant, K., Rabilu, S. A., Rashid, A., & Zsigmond, L. (2026) · Plant Cell Reports · doi:10.1007/s00299-026-03891-w · Cited by 3
Genome editing in rice: New paths for an old crop
Nizolli, V. O., de Oliveira, V. F., da Maia, L. C., Pegoraro, C., & de Oliveira, A. C. (2023) · CABI Reviews · doi:10.1079/cabireviews.2023.0008 · Cited by 2
Targeted Activation of Arabidopsis Genes by a Potent CRISPR–Act3.0 System
Pan, C., & Qi, Y. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3354-0_3 · Cited by 2
Self-citationAssembly of TALEN and mTALE-Act for Plant Genome Engineering
Malzahn, A. A., & Qi, Y. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-1201-9_15 · Cited by 2
Self-citationDesigner plants for climate-resilient phytoremediation
Pandey, V. C., Gajić, G., Sharma, P., & Roy, M. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-823831-8.00007-4 · Cited by 2
The Use of CRISPR Technologies for Crop Improvement in Maize
Young, J. K., Barone, P., Gasior, S. L., Jones, S., Djukanovic, V., & Simon, M. K. (2022) · Springer eBooks · doi:10.1007/978-981-19-0600-8_13 · Cited by 2
Tang, Y., Wang, C., Wang, F., Li, M., Fang, Y., Ji, Z., & Zhao, K. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.924645 · Cited by 1
Wolella, E. K., Zhen, C., Li, M., Xia, D., Zhang, J., Duan, L., Liu, L., Li, Z., & Zhang, J. (2025) · Plants · doi:10.3390/plants14101492 · Cited by 1
Enhancing gene therapy vectors manufacturing: CEBPA as a master regulator of rAAV biogenesis
Moura, F., Antunes, M., Correia, R., Roldão, A., Gomes‐Alves, P., Alves, P. M., & Escandell, J. M. (2026) · Journal of Biological Engineering · doi:10.1186/s13036-026-00622-3 · Cited by 1
CRISPR-Cas-Mediated Gene Editing in Sorghum: Recent Advances, Challenges, and Prospects
Wang, Z., Massel, K., Wang, J., Jiao, Z., Godwin, I. D., Lv, P., & Liu, G. (2026) · Sustainability Sciences in Asia and Africa · doi:10.1007/978-981-95-6722-5_11 · Cited by 1
Compact Cas12 Systems for Multiplex Engineering of Plant Abiotic‐Stress Networks
Thabet, S. G., Alwutayd, K. M., Elkelish, A., Safhi, F. A., Dawood, M. F. A., & Alqudah, A. M. (2026) · Advanced Genetics · doi:10.1002/ggn2.202500067 · Cited by 1
Misexpression Approaches for the Manipulation of Flower Development
Xu, Y., Gan, E., & Ito, T. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3299-4_21 · Cited by 1
Metadata
- Authors
- Lowder, L. G., Zhou, J., Zhang, Y., Malzahn, A., Zhong, Z., Hsieh, T.-F., Voytas, D. F., Zhang, Y., & Qi, Y.
- Affiliations
- East Carolina University (US)
- University of Electronic Science and Technology of China (CN)
- University of Maryland, College Park (US)
- North Carolina State University (US)
- University of Minnesota (US)
- Corresponding author
- Qi, Yiping
- Venue
- Molecular Plant
- Publisher
- Elsevier BV
- Published
- 2018-02
- Volume
- 11
- Issue
- 2
- Pages
- 245-256
- ISSN
- 1674-2052 (ISSN-L)
- OpenAlex
- W2768119869
- Cited by
- 232
- References
- 81
- Citations by year
- 2026: 20 · 2025: 22 · 2024: 13 · 2023: 28 · 2022: 45 · 2021: 29 · 2020: 32 · 2019: 36 · 2018: 7
- FWCI
- 8.37
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Plant (publishedVersion)
- PubMed (publishedVersion)
- Funders
- East Carolina University
- University of Maryland-College Park
- North Carolina Biotechnology Center
- Syngenta Biotechnology (2016-CFG-8003)
- Sichuan Youth Science and Technology Foundation (2017JQ0005)
- National Science Foundation of China (31771486)
- Fundamental Research Funds for the Central Universities (ZYGX2016J119)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- transcriptional activation, transcription activator-like effectors, positive feedback loop, gene regulatory networks, Golden Gate cloning, Gateway cloning, plant genome editing
- MeSH terms
- Transcription Factors, Transcriptional Activation, Gene Expression Regulation, Plant, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review