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Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems
Open accessAbstract
User-friendly tools for robust transcriptional activation of endogenous genes are highly demanded in plants. We previously showed that a dCas9-VP64 system consisting of the deactivated CRISPR-associated protein 9 (dCas9) fused with four tandem repeats of the transcriptional activator VP16 (VP64) could be used for transcriptional activation of endogenous genes in plants. In this study, we developed a second generation of vector systems for enhanced transcriptional activation in plants. We tested multiple strategies for dCas9-based transcriptional activation, and found that simultaneous recruitment of VP64 by dCas9 and a modified guide RNA scaffold gRNA2.0 (designated CRISPR-Act2.0) yielded stronger transcriptional activation than the dCas9-VP64 system. Moreover, we developed a multiplex transcription activator-like effector activation (mTALE-Act) system for simultaneous activation of up to four genes in plants. Our results suggest that mTALE-Act is even more effective than CRISPR-Act2.0 in most cases tested. In addition, we explored tissue-specific gene activation using positive feedback loops. Interestingly, our study revealed that certain endogenous genes are more amenable than others to transcriptional activation, and tightly regulated genes may cause target gene silencing when perturbed by activation probes. Hence, these new tools could be used to investigate gene regulatory networks and their control mechanisms. Assembly of multiplex CRISPR-Act2.0 and mTALE-Act systems are both based on streamlined and PCR-independent Golden Gate and Gateway cloning strategies, which will facilitate transcriptional activation applications in both dicots and monocots.
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“In rice, simultaneous multigene activation is possible using the CRISPR activation (CRISPRa) system (Lowder et al., 2018).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Lowder et al., 2018
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Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana
Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 22,014
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Generation of germline-competent induced pluripotent stem cells
Okita, K., Ichisaka, T., & Yamanaka, S. (2007) · Nature · doi:10.1038/nature05934 · Cited by 4,438
Contrasting Mechanisms of Defense Against Biotrophic and Necrotrophic Pathogens
Glazebrook, J. (2005) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.43.040204.135923 · Cited by 4,380
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
Napoli, C. A., Lemieux, C., & Jorgensen, R. A. (1990) · The Plant Cell · doi:10.1105/tpc.2.4.279 · Cited by 2,892
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Konermann, S., Brigham, M. D., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., et al. (2015) · Nature · doi:10.1038/nature14136 · Cited by 2,825
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Boch, J., Scholze, H., Schornack, S., Landgraf, A., Hahn, S., Kay, S., Lahaye, T., Nickstadt, A., & Bonas, U. (2009) · Science · doi:10.1126/science.1178811 · Cited by 2,470
Ma, X., Zhang, Q., Zhu, Q., Liu, W., Chen, Y., Qiu, R., Wang, B., Yang, Z., Li, H., Lin, Y., et al. (2015) · Molecular Plant · doi:10.1016/j.molp.2015.04.007 · Cited by 2,369
A Simple Cipher Governs DNA Recognition by TAL Effectors
Moscou, M., & Bogdanove, A. J. (2009) · Science · doi:10.1126/science.1178817 · Cited by 2,050
Čermák, T., Doyle, E., Christian, M. L., Wang, L., Zhang, Y., Schmidt, C. L., Baller, J. A., Somia, N. V., Bogdanove, A. J., & Voytas, D. F. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr218 · Cited by 2,034
Self-citationMali, P., Aach, J., Stranges, P. B., Esvelt, K. M., Moosburner, M., Kosuri, S., Yang, L., & Church, G. M. (2013) · Nature Biotechnology · doi:10.1038/nbt.2675 · Cited by 1,752
A CRISPR/Cas9 toolkit for multiplex genome editing in plants
Xing, H.-L., Dong, L., Wang, Z.-P., Zhang, H.-Y., Han, C.-Y., Liu, B., Wang, X.-C., & Chen, Q.-J. (2014) · BMC Plant Biology · doi:10.1186/s12870-014-0327-y · Cited by 1,684
Highly efficient Cas9-mediated transcriptional programming
Chavez, A., Scheiman, J., Vora, S., Pruitt, B. W., Tuttle, M., P R Iyer, E., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3312 · Cited by 1,622
A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging
Tanenbaum, M. E., Gilbert, L. A., Qi, L. S., Weissman, J. S., & Vale, R. D. (2014) · Cell · doi:10.1016/j.cell.2014.09.039 · Cited by 1,611
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
Borevitz, J., Xia, Y., Blount, J. W., Dixon, R. A., & Lamb, C. J. (2000) · The Plant Cell · doi:10.1105/tpc.12.12.2383 · Cited by 1,451
RNA-guided gene activation by CRISPR-Cas9-based transcription factors
Perez-Pinera, P., Kocak, D. D., Vockley, C. M., Adler, A. F., Kabadi, A. M., Polstein, L. R., Thakore, P. I., Glass, K. A., Ousterout, D. G., Leong, K. W., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2600 · Cited by 1,328
GAL4-VP16 is an unusually potent transcriptional activator
Sadowski, I., Ma, J., Triezenberg, S., & Ptashne, M. (1988) · Nature · doi:10.1038/335563a0 · Cited by 1,298
CRISPR RNA–guided activation of endogenous human genes
Maeder, M. L., Linder, S. J., Cascio, V. M., Fu, Y., Ho, Q. H., & Joung, J. K. (2013) · Nature Methods · doi:10.1038/nmeth.2598 · Cited by 1,249
Butelli, E., Titta, L., Giorgio, M., Mock, H.-P., Matros, A., Peterek, S., Schijlen, E. G. W. M., Hall, R. D., Bovy, A. G., Luo, J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt.1506 · Cited by 1,238
van der Krol, A. R., Mur, L. A. J., Beld, M., Mol, J. N. M., & Stuitje, A. R. (1990) · The Plant Cell · doi:10.1105/tpc.2.4.291 · Cited by 1,204
Szymczak, A. L., Workman, C. J., Wang, Y., Vignali, K. M., Dilioglou, S., Vanin, E. F., & Vignali, D. A. A. (2004) · Nature Biotechnology · doi:10.1038/nbt957 · Cited by 1,059
A glucocorticoid‐mediated transcriptional induction system in transgenic plants
Aoyama, T., & Chua, N. (1997) · The Plant Journal · doi:10.1046/j.1365-313x.1997.11030605.x · Cited by 940
Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds
Zalatan, J. G., Lee, M. E., Almeida, R., Gilbert, L. A., Whitehead, E. H., La Russa, M., Tsai, J. C., Weissman, J. S., Dueber, J. E., Qi, L. S., et al. (2015) · Cell · doi:10.1016/j.cell.2014.11.052 · Cited by 938
Control of Jasmonate Biosynthesis and Senescence by miR319 Targets
Schommer, C., Palatnik, J. F., Aggarwal, P., Chételat, A., Cubas, P., Farmer, E. E., Nath, U., & Weigel, D. (2008) · PLoS Biology · doi:10.1371/journal.pbio.0060230 · Cited by 881
Cheng, A. W., Wang, H., Yang, H., Shi, L., Yarden Katz, A., Theunissen, T. W., Rangarajan, S., Shivalila, C., Dadon, D. B., & Jaenisch, R. (2013) · Cell Research · doi:10.1038/cr.2013.122 · Cited by 768
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation
Lowder, L. G., Zhang, D., Baltes, N. J., Paul, J. W., Tang, X., Zheng, X., Voytas, D. F., Hsieh, T.-F., Zhang, Y., & Qi, Y. (2015) · Plant Physiology · doi:10.1104/pp.15.00636 · Cited by 707
Self-citationA CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants
Tang, X. D., Lowder, L. G., Zhang, T., Malzahn, A. A., Zheng, X., Voytas, D. F., Zhong, Z., Chen, Y., Ren, Q., Li, Q., et al. (2017) · Nature Plants · doi:10.1038/nplants.2017.18 · Cited by 619
Self-citationThe repair of double-strand breaks in plants: mechanisms and consequences for genome evolution
Puchta, H. (2004) · Journal of Experimental Botany · doi:10.1093/jxb/eri025 · Cited by 603
Genome Engineering with Targetable Nucleases
Carroll, D. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035418 · Cited by 568
DNA Replicons for Plant Genome Engineering
Baltes, N. J., Gil‐Humanes, J., Čermák, T., Atkins, P., & Voytas, D. F. (2014) · The Plant Cell · doi:10.1105/tpc.113.119792 · Cited by 552
Self-citationBeerli, R. R., Segal, D. J., Dreier, B., & Barbas, C. F. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.25.14628 · Cited by 539
Molecular mechanisms of DNA double-strand break repair
Kanaar, R., Hoeijmakers, J. H. J., & van Gent, D. C. (1998) · Trends in Cell Biology · doi:10.1016/s0962-8924(98)01383-x · Cited by 527
Comparison of Cas9 activators in multiple species
Chavez, A., Tuttle, M. L., Pruitt, B. W., Ewen‐Campen, B., Chari, R., Ter‐Ovanesyan, D., Haque, S. J., Cecchi, R. J., Kowal, E. J. K., Buchthal, J., et al. (2016) · Nature Methods · doi:10.1038/nmeth.3871 · Cited by 521
CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications
Ma, X., Zhu, Q., Chen, Y., & Liu, Y. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.04.009 · Cited by 497
Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering
Zhang, Y., Zhang, F., Li, X., Baller, J. A., Qi, Y., Starker, C. G., Bogdanove, A. J., & Voytas, D. F. (2012) · PLANT PHYSIOLOGY · doi:10.1104/pp.112.205179 · Cited by 487
Self-citationPlant Genome Engineering with Sequence-Specific Nucleases
Voytas, D. F. (2013) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042811-105552 · Cited by 462
Self-citationTranscriptional gene silencing in plants: targets, inducers and regulators
Vaucheret, H., & Fagard, M. (2001) · Trends in Genetics · doi:10.1016/s0168-9525(00)02166-1 · Cited by 368
A mechanism for synergistic activation of a mammalian gene by GAL4 derivatives
Carey, M., Lin, Y.-S., Green, M. R., & Ptashne, M. (1990) · Nature · doi:10.1038/345361a0 · Cited by 361
Suppression of beta‐1,3‐glucanase transgene expression in homozygous plants.
de Carvalho‐Niebel, F., Gheysen, G., Kushnir, S., MONTAGU, M. V., Inzé, D., & Castresana, C. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05324.x · Cited by 304
Induced α Helix in the VP16 Activation Domain upon Binding to a Human TAF
Uesugi, M., Nyanguile, O., Lu, H., Levine, A. J., & Verdine, G. L. (1997) · Science · doi:10.1126/science.277.5330.1310 · Cited by 284
Morbitzer, R., Römer, P., Boch, J., & Lahaye, T. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1013133107 · Cited by 283
Synthetic nucleases for genome engineering in plants: prospects for a bright future
Puchta, H., & Fauser, F. (2013) · The Plant Journal · doi:10.1111/tpj.12338 · Cited by 275
Bacteria-responsive microRNAs regulate plant innate immunity by modulating plant hormone networks
Zhang, W., Gao, S., Zhou, X., Padmanabhan, C., Chen, Z., Zhou, X., Zhang, X., Fromuth, N., Coutino, G., Coffey, M. D., et al. (2010) · Plant Molecular Biology · doi:10.1007/s11103-010-9710-8 · Cited by 260
Synergistic and tunable human gene activation by combinations of synthetic transcription factors
Pérez‐Piñera, P., Ousterout, D. G., Brunger, J. M., Farin, A. M., Glass, K. A., Guilak, F., Crawford, G. E., Hartemink, A. J., & Gersbach, C. A. (2013) · Nature Methods · doi:10.1038/nmeth.2361 · Cited by 242
Zhang, C., Ding, Z., Wu, K., Yang, L., Li, Y., Yang, Z., Shi, S., Liu, X., Zhao, S., Yang, Z., et al. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.06.014 · Cited by 233
Use of designer nucleases for targeted gene and genome editing in plants
Weeks, D. P., Spalding, M. H., & Yang, B. (2015) · Plant Biotechnology Journal · doi:10.1111/pbi.12448 · Cited by 231
CRISPR-Cas9 Based Genome Editing Reveals New Insights into MicroRNA Function and Regulation in Rice
Zhou, J., Deng, K., Cheng, Y., Zhong, Z., Tian, L., Tang, X. D., Tang, A., Zheng, X., Zhang, T., Qi, Y., et al. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01598 · Cited by 204
Self-citationBultmann, S., Morbitzer, R., Schmidt, C. S., Thanisch, K., Spada, F., Elsäesser, J., Lahaye, T., & Leonhardt, H. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks199 · Cited by 184
A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants
Tang, X. D., Zheng, X., Qi, Y., Zhang, D., Cheng, Y., Tang, A., Voytas, D. F., & Zhang, Y. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.05.001 · Cited by 184
Self-citationRobust, synergistic regulation of human gene expression using TALE activators
Maeder, M. L., Linder, S. J., Reyon, D., Angstman, J. F., Fu, Y., Sander, J. D., & Joung, J. K. (2013) · Nature Methods · doi:10.1038/nmeth.2366 · Cited by 182
TAL effectors are remote controls for gene activation
Scholze, H., & Boch, J. (2011) · Current Opinion in Microbiology · doi:10.1016/j.mib.2010.12.001 · Cited by 166
Hart, C. M., Fischer, B., Neuhaus, J., & Meins, F. (1992) · Molecular and General Genetics MGG · doi:10.1007/bf00279359 · Cited by 165
Zhao, W., Li, Z., Fan, J., Hu, C., Yang, R., Qi, X., Chen, H., Zhao, F., & Wang, S. (2015) · Journal of Experimental Botany · doi:10.1093/jxb/erv238 · Cited by 159
Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein
Mahfouz, M. M., Li, L., Piatek, M. J., Fang, X., Mansour, H., Bangarusamy, D. K., & Zhu, J. (2011) · Plant Molecular Biology · doi:10.1007/s11103-011-9866-x · Cited by 157
The EDLL motif: a potent plant transcriptional activation domain from AP2/ERF transcription factors
Tiwari, S. B., Belachew, A., Ma, S. F., Young, M., Ade, J., Shen, Y., Marion, C. M., Holtan, H., Bailey, A. M., Stone, J. K., et al. (2012) · The Plant Journal · doi:10.1111/j.1365-313x.2012.04935.x · Cited by 156
Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas
Schiml, S., & Puchta, H. (2016) · Plant Methods · doi:10.1186/s13007-016-0103-0 · Cited by 151
Doyle, E., Stoddard, B. L., Voytas, D. F., & Bogdanove, A. J. (2013) · Trends in Cell Biology · doi:10.1016/j.tcb.2013.04.003 · Cited by 135
Self-citationEngineering disease resistance in plants
Stuiver, M. H., & Custers, J. H. H. V. (2001) · Nature · doi:10.1038/35081200 · Cited by 134
Zinc-finger-based artificial transcription factors and their applications
Sera, T. (2009) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.03.012 · Cited by 112
Palauqui, J. C., Elmayan, T., de Borne, F. D., Crété, P., Charles, C., & Vaucheret, H. (1996) · PLANT PHYSIOLOGY · doi:10.1104/pp.112.4.1447 · Cited by 110
Co-suppression of nitrate reductase host genes and transgenes in transgenic tobacco plants
de Borne, F. D., Vincentz, M., Chupeau, Y., & Vaucheret, H. (1994) · Molecular and General Genetics MGG · doi:10.1007/bf00279570 · Cited by 108
Resources for the design of CRISPR gene editing experiments
Graham, D. B., & Root, D. E. (2015) · Genome biology · doi:10.1186/s13059-015-0823-x · Cited by 108
Genetic engineering for increasing fungal and bacterial disease resistance in crop plants
Wally, O. S. D., & Punja, Z. K. (2010) · GM Crops · doi:10.4161/gmcr.1.4.13225 · Cited by 107
Advances in zinc finger engineering
Choo, Y., & Isalan, M. (2000) · Current Opinion in Structural Biology · doi:10.1016/s0959-440x(00)00107-x · Cited by 95
Rapid Evolution of Manifold CRISPR Systems for Plant Genome Editing
Lowder, L. G., Malzahn, A. A., & Qi, Y. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01683 · Cited by 88
Self-citationTALE-mediated modulation of transcriptional enhancers in vivo
Crocker, J., & Stern, D. L. (2013) · Nature Methods · doi:10.1038/nmeth.2543 · Cited by 80
Multiplexed Transcriptional Activation or Repression in Plants Using CRISPR-dCas9-Based Systems
Lowder, L. G., Paul, J. W., & Qi, Y. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7125-1_12 · Cited by 80
Self-citationGupta, M., DeKelver, R. C., Palta, A., Clifford, C., Gopalan, S. M., Miller, J. C., Novak, S., DeSloover, D., Gachotte, D. J., Connell, J., et al. (2012) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2012.00695.x · Cited by 73
A Putative RNA-Binding Protein Positively Regulates Salicylic Acid–Mediated Immunity in Arabidopsis
Qi, Y., Tsuda, K., Joe, A., Sato, M., Nguyen, L. V., Glazebrook, J., Alfano, J. R., Cohen, J. D., & Katagiri, F. (2010) · Molecular Plant-Microbe Interactions · doi:10.1094/mpmi-05-10-0106 · Cited by 54
Self-citationEngineering metabolic pathways in plants by multigene transformation
Zorrilla‐López, U., Masip, G., Arjó, G., Bai, C., Banakar, R., Bassié, L., Berman, J., Farré, G., Miralpeix, B., Pérez‐Massot, E., et al. (2013) · The International Journal of Developmental Biology · doi:10.1387/ijdb.130162pc · Cited by 47
Mitsuhara, I., Shirasawa-Seo, N., Iwai, T., Nakamura, S., Honkura, R., & Ohashi, Y. (2002) · Genetics · doi:10.1093/genetics/160.1.343 · Cited by 45
Activation domains for controlling plant gene expression using designed transcription factors
Li, J., Blue, R., Zeitler, B., Strange, T. L., Pearl, J. R., Huizinga, D. H., Evans, S., Gregory, P. D., Urnov, F. D., & Petolino, J. F. (2013) · Plant Biotechnology Journal · doi:10.1111/pbi.12057 · Cited by 40
Regulation of Gene Expression in Arabidopsis thaliana by Artificial Zinc Finger Chimeras
Sanchez, J.-P., Ullman, C., Moore, M., Choo, Y., & Chua, N. (2002) · Plant and Cell Physiology · doi:10.1093/pcp/pcf186 · Cited by 38
Dzivenu, O. K., Park, H. H., & Wu, H. (2004) · Protein Expression and Purification · doi:10.1016/j.pep.2004.07.016 · Cited by 36
Velten, J. P., Çakir, C., Youn, E., Chen, J., & Cazzonelli, C. I. (2012) · PLoS ONE · doi:10.1371/journal.pone.0030141 · Cited by 27
Synthetic TAL effectors for targeted enhancement of transgene expression in plants
Liu, W., Rudis, M. R., Peng, Y., Mazarei, M., Millwood, R. J., Yang, J., Xu, W., Chesnut, J. D., & Stewart, C. N. (2013) · Plant Biotechnology Journal · doi:10.1111/pbi.12150 · Cited by 20
Plant Gene Regulation Using Multiplex CRISPR-dCas9 Artificial Transcription Factors
Lowder, L. G., Malzahn, A. A., & Qi, Y. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7315-6_12 · Cited by 18
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Metadata
- Authors
- Lowder, L. G., Zhou, J., Zhang, Y., Malzahn, A., Zhong, Z., Hsieh, T.-F., Voytas, D. F., Zhang, Y., & Qi, Y.
- Affiliations
- East Carolina University (US)
- University of Electronic Science and Technology of China (CN)
- University of Maryland, College Park (US)
- North Carolina State University (US)
- University of Minnesota (US)
- Corresponding author
- Qi, Yiping
- Venue
- Molecular Plant
- Publisher
- Elsevier BV
- Published
- 2018-02
- Volume
- 11
- Issue
- 2
- Pages
- 245-256
- ISSN
- 1674-2052 (ISSN-L)
- OpenAlex
- W2768119869
- Cited by
- 232
- References
- 81
- Citations by year
- 2026: 20 · 2025: 22 · 2024: 13 · 2023: 28 · 2022: 45 · 2021: 29 · 2020: 32 · 2019: 36 · 2018: 7
- FWCI
- 8.37
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Plant (publishedVersion)
- PubMed (publishedVersion)
- Funders
- East Carolina University
- University of Maryland-College Park
- North Carolina Biotechnology Center
- Syngenta Biotechnology (2016-CFG-8003)
- Sichuan Youth Science and Technology Foundation (2017JQ0005)
- National Science Foundation of China (31771486)
- Fundamental Research Funds for the Central Universities (ZYGX2016J119)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- transcriptional activation, transcription activator-like effectors, positive feedback loop, gene regulatory networks, Golden Gate cloning, Gateway cloning, plant genome editing
- MeSH terms
- Transcription Factors, Transcriptional Activation, Gene Expression Regulation, Plant, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems
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