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Cited by 5Photosynthetic Processes and MechanismsATP Synthase and ATPases ResearchAdvanced Electron Microscopy Techniques and Applications

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Architecture of the ATP-driven motor for protein import into chloroplasts

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Wang, Ning, Xing, Jiale, Su, Xiaodong, Pan, Junting, Chen, Hui, Shi, Lifang, Si, Long, Yang, Wenqiang, Li, Mei·2024·Molecular Plant
DOI 10.1016/j.molp.2024.09.010

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Abstract

Thousands of nuclear-encoded proteins are transported into chloroplasts through the TOC-TIC translocon that spans the chloroplast envelope membranes. A motor complex pulls the translocated proteins out of the TOC-TIC complex into the chloroplast stroma by hydrolyzing ATP. The Orf2971-FtsHi complex has been suggested to serve as the ATP-hydrolyzing motor in Chlamydomonas reinhardtii, but little is known about its architecture and assembly. Here, we report the 3.2-Å resolution structure of the Chlamydomonas Orf2971-FtsHi complex. The 20-subunit complex spans the chloroplast inner envelope, with two bulky modules protruding into the intermembrane space and stromal matrix. Six subunits form a hetero-hexamer that potentially provides the pulling force through ATP hydrolysis. The remaining subunits, including potential enzymes/chaperones, likely facilitate the complex assembly and regulate its proper function. Taken together, our results provide the structural foundation for a mechanistic understanding of chloroplast protein translocation.

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Metadata

Authors
Wang, N., Xing, J., Su, X., Pan, J., Chen, H., Shi, L., Si, L., Yang, W., & Li, M.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
  • Yang, Wenqiang
  • Li, Mei
ORCID
Venue
Molecular Plant
Publisher
Elsevier BV
Published
2024-11
Volume
17
Issue
11
Pages
1702-1718
ISSN
1674-2052 (ISSN-L)
DOI
10.1016/j.molp.2024.09.010
OpenAlex
W4402828531
Cited by
5
References
4
Citations by year
2026: 3 · 2025: 2
FWCI
0.68
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecular Plant (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • Natural Science Foundation of Shandong Province (ZR2023ZD30)
  • Chinese Academy of Sciences (Y2022038, XDB37020101)
  • National Key Research and Development Program of China (2021YFA0910800)
  • Ministry of Science and Technology of the People&apos;s Republic of China
  • National Natural Science Foundation of China (31930064)
  • Biofyzikální Ústav, Akademie Věd České Republiky
  • Youth Innovation Promotion Association
  • University of Chinese Academy of Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
Keywords
chloroplast protein import, Chlamydomonas reinhardtii, chloroplast envelope, protein translocation, complex architecture, nuclear-encoded chloroplast proteins, molecular chaperones
MeSH terms
Adenosine Triphosphate, Chloroplasts, Plant Proteins, Chlamydomonas reinhardtii, Protein Transport

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