The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
Engineering a multifunctional 3D-printed PLA-collagen-minocycline-nanoHydroxyapatite scaffold with combined antimicrobial and osteogenic effects for bone regeneration
Abstract
3D-printing and additive manufacturing can be powerful techniques to design customized structures and produce synthetic bone grafts with multifunctional effects suitable for bone repair. In our work we aimed the development of novel multifunctionalized 3D printed poly(lactic acid) (PLA) scaffolds with bioinspired surface coatings able to reduce bacterial biofilm formation while favoring human bone marrow-derived mesenchymal stem cells (hMSCs) activity. For that purpose, 3D printing was used to prepare PLA scaffolds that were further multifunctionalized with collagen (Col), minocycline (MH) and bioinspired citrate- hydroxyapatite nanoparticles (cHA). PLA-Col-MH-cHA scaffolds provide a closer structural support approximation to native bone architecture with uniform macroporous, adequate wettability and an excellent compressive strength. The addition of MH resulted in an adequate antibiotic release profile that by being compatible with local drug delivery therapy was translated into antibacterial activities against Staphylococcus aureus, a main pathogen associated to bone-related infections. Subsequently, the hMSCs response to these scaffolds revealed that the incorporation of cHA significantly stimulated the adhesion, proliferation and osteogenesis-related gene expression (RUNX2, OCN and OPN) of hMSCs. Furthermore, the association of a bioinspired material (cHA) with the antibiotic MH resulted in a combined effect of an enhanced osteogenic activity. These findings, together with the antibiofilm activity depicted strengthen the appropriateness of this 3D-printed PLA-Col-MH-cHA scaffold for future use in bone repair. By targeting bone repair while mitigating the typical infections associated to bone implants, our 3D scaffolds deliver an integrated strategy with the combined effects further envisaging an increase in the success rate of bone-implanted devices.
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“Bioactive compound along with nano-HA/collagen enhanced the osteogenic properties and mechanical property.[107]”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [107]
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Balla, E. D., Daniilidis, V., Karlioti, G., Kalamas, T., Stefanidou, M., Bikiaris, N. D., Vlachopoulos, A., Koumentakou, I., & Bikiaris, D. Ν. (2021) · Polymers · doi:10.3390/polym13111822 · Cited by 697
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Biomaterials and nanomedicine for bone regeneration: Progress and future prospects
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Metadata
- Authors
- Martin, V., Ribeiro, I. A., Alves, M. M., Gonçalves, L., Claudio, R. A., Grenho, L., Fernandes, M. H., Gomes, P., Santos, C. F., & Bettencourt, A. F.
- Affiliations
- University of Lisbon (PT)
- Universidade do Porto (PT)
- Corresponding author
- Santos, Catarina F.
- Bettencourt, Ana F.
- ORCID
- Venue
- Materials Science and Engineering: C
- Publisher
- Elsevier BV
- Published
- 2019-08
- Volume
- 101
- Pages
- 15-26
- ISSN
- 0928-4931 (ISSN-L)
- OpenAlex
- W2923561240
- Cited by
- 181
- References
- 59
- Citations by year
- 2026: 8 · 2025: 16 · 2024: 32 · 2023: 25 · 2022: 26 · 2021: 39 · 2020: 30 · 2019: 4
- FWCI
- 8.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Materials Science and Engineering C (publishedVersion)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
- Funders
- Fundação para a Ciência e a Tecnologia (SFRH/BPD/76646/2011, UID/QUI/00100/2013, PT2020 UID/QUI/50006/2013, UID/DTP/04138/2014)
- Ministério da Educação e Ciência
- European Commission (POCI/01/0145/FEDER/007265)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Industrial and Manufacturing Engineering › Additive Manufacturing and 3D Printing Technologies
- Keywords
- 3D-printed scaffolds, polylactic acid, collagen, minocycline, bone regeneration, bone grafting, osteogenic differentiation, mesenchymal stem cells, antibacterial activity, biofilm inhibition, Staphylococcus aureus, antibiotic release, local drug delivery
- MeSH terms
- Adsorption, Anti-Bacterial Agents, Bone Regeneration, Collagen, Gene Expression Regulation, Humans, Microbial Sensitivity Tests, Minocycline, Osteogenesis, Polyesters, Staphylococcus aureus, Osseointegration, Durapatite, Biofilms, Tissue Engineering, Nanoparticles, Tissue Scaffolds, Mesenchymal Stem Cells, Drug Liberation, Printing, Three-Dimensional
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