The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Size-dependent cellular uptake of exosomes
Open accessAbstract
The ability of exosomes to elicit specific cellular responses suggests that they may be increasingly used as therapeutics. Their vesicular nature makes them suitable as potential nanocarriers for drugs or nucleic acids delivery. Here we address the question whether the method of preparation of enriched exosomal fractions can affect their uptake by cells and their ability to trigger a response. We compared ultracentrifugation and polymer-based precipitation methods on supernatants of glioma-associated stem cells isolated from a high-grade glioma patient. We determined particle size distributions after purification and their correlation with uptake, proliferation and migration in glioblastoma cell cultures. Our findings indicate that polymer-based precipitation leads to smaller particle size distributions, faster uptake by target cells and increased cellular motility. The different effect that isolation method-dependent populations of particles have on cell motility suggests their size distribution could also profoundly affect exosomes therapeutic potential.
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Cites
Extracellular vesicles: Exosomes, microvesicles, and friends
Raposo, G., & Stoorvogel, W. (2013) · Journal of Cell Biology · doi:10.1083/jcb.201211138 · Cited by 8,099
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Kowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521230113 · Cited by 3,426
Routes and mechanisms of extracellular vesicle uptake
Mulcahy, L. A., Pink, R. C., & Carter, D. R. F. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24641 · Cited by 2,832
Christianson, H. C., Svensson, K. J., van Kuppevelt, T. H., Li, J.-P., & Belting, M. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1304266110 · Cited by 903
Cited by
The Exosome Total Isolation Chip
Liu, F., Vermesh, O., Mani, V., Ge, T. J., Madsen, S. J., Sabour, A., Hsu, E.-C., Gowrishankar, G., Kanada, M., Jokerst, J. V., et al. (2017) · ACS Nano · doi:10.1021/acsnano.7b04878 · Cited by 396
Extracellular vesicles in cancer nanomedicine
Tarasov, V. V., Svistunov, A. A., Chubarev, V. N., Dostdar, S. A., Sokolov, A. V., Brzecka, A., Sukocheva, O., Neganova, M. E., Klochkov, S. G., Somasundaram, S. G., et al. (2021) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2019.08.017 · Cited by 97
Engineering strategies for customizing extracellular vesicle uptake in a therapeutic context
Esmaeili, A., Alini, M., Baghaban Eslaminejad, M., & Hosseini, S. (2022) · Stem Cell Research & Therapy · doi:10.1186/s13287-022-02806-2 · Cited by 94
Engineering exosomes for bone defect repair
Ma, S., Zhang, Y., Li, S., Li, A., Li, Y., & Pei, D. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.1091360 · Cited by 38
Metadata
- Authors
- Caponnetto, F., Manini, I., Skrap, M., Palmai-Pallag, T., Di Loreto, C., Beltrami, A. P., Cesselli, D., & Ferrari, E.
- Venue
- Nanomedicine: Nanotechnology, Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2017-04
- Volume
- 13
- Issue
- 3
- Pages
- 1011-1020
- ISSN
- 1549-9634 (ISSN-L)
- OpenAlex
- W2567617942
- Cited by
- 179
- References
- 5
- FWCI
- 5.27
- Type
- journal article
- Language
- en
- Open access
- green
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- Caponnetto, F., Manini, I., Skrap, M., Palmai-Pallag, T., Di Loreto, C., Beltrami, A. P., Cesselli, D., & Ferrari, E. (2017). Size-dependent cellular uptake of exosomes. Nanomedicine: Nanotechnology, Biology and Medicine. https://doi.org/10.1016/j.nano.2016.12.009
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
