Detecting axon damage in spinal cord from a mouse model of multiple sclerosis
Open accessAbstract
In the current study, the feasibility and reproducibility of in vivo diffusion tensor imaging (DTI) of the spinal cord in normal mice are illustrated followed by its application to mice with experimental allergic encephalomyelitis (EAE) to detect and differentiate axon and myelin damage. Axial diffusivity, describing water movement along the axonal fiber tract, in all regions of spinal cord white matter from EAE-affected C57BL/6 mice was significantly decreased compared to normal mice, whereas there was no statistically significant change in radial diffusivity, describing water movement across the fiber tract. Furthermore, a direct comparison between DTI and histology from a single mouse demonstrated a decrease in axial diffusivity that was supported by widespread staining of antibody against beta-amyloid precursor protein. Regionally elevated radial diffusivity corresponded with locally diminished Luxol fast blue staining in the same tissue from the EAE mouse cord. Our findings suggest that axonal damage is more widespread than myelin damage in the spinal cord white matter of mice with EAE and that in vivo DTI may provide a sensitive and specific measure of white matter injury.
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DTI successfully detects axon and myelin injury through decreased axial diffusivity and increased radial diffusivity in animal models of CNS diseases and injuries.
“Diffusion tensor imaging (DTI) successfully detects axon and myelin injury through decreased axial diffusivity (λ∥, parallel to white matter tract) and increased radial diffusivity (λ⊥, perpendicular to white matter tract) in animal models of central nervous system (CNS) diseases and injuries (Song et al., 2002, Kim et al., 2006, Sun et al., 2006, DeBoy et al., 2007).”
From Quantifying white matter tract diffusion parameters in the presence of increased extra-fiber cellularity and vasogenic edema (2014) · cites this paper as Kim et al., 2006
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“…(λ║, parallel to white matter tract) and increased radial diffusivity (λ⊥, perpendicular to white matter tract) in animal models of central nervous system (CNS) diseases and injuries (DeBoy et al., 2007; Kim et al., 2006; Song et al., 2002; Campus Box 8227, Washington Ave, St Louis, MO 63110, USA.”
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- Authors
- Kim, J. H., Budde, M. D., Liang, H.-F., Klein, R. S., Russell, J. H., Cross, A. H., & Song, S.-K.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Song, Sheng-Kwei
- Venue
- Neurobiology of Disease
- Publisher
- Elsevier BV
- Published
- 2006-03
- Volume
- 21
- Issue
- 3
- Pages
- 626-632
- ISSN
- 0969-9961 (ISSN-L)
- OpenAlex
- W1964565539
- Cited by
- 235
- References
- 6
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 1 · 2021: 6 · 2020: 7 · 2019: 8 · 2018: 12 · 2017: 7 · 2016: 12 · 2015: 10 · 2014: 21 · 2013: 16 · 2012: 16
- FWCI
- 12.01
- Type
- journal article
- Language
- en
- Open access
- green
- doaj.org
- Licence
- Locations
- Neurobiology of Disease (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Cancer Institute (R24CA083060, R24-CA83060)
- National Institute of Neurological Disorders and Stroke (K02NS045607)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Keywords
- axonal injury, spinal cord white matter, multiple sclerosis, experimental autoimmune encephalomyelitis, diffusion tensor imaging, axial diffusivity, radial diffusivity, myelin damage, amyloid precursor protein, Luxol fast blue, in vivo MRI, mouse model
- MeSH terms
- Animals, Axons, Diffusion, Encephalomyelitis, Autoimmune, Experimental, Female, Mice, Inbred C57BL, Multiple Sclerosis, Myelin Sheath, Spinal Cord, Reproducibility of Results, Diffusion Magnetic Resonance Imaging, Mice
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