Cellulase Production from Aspergillus Niger MS82: Effect of Temperature and PH
Abstract
Fungal cellulases are well-studied enzymes and are used in various industrial processes. Much of the knowledge of enzymatic depolymerization of cellulosic material has come from Trichoderma cellulase system. Species of Trichoderma can produce substantial amounts of endoglucanase and exoglucanase but very low levels of b-glucosidase. This deficiency necessitates screening of fungi for cellulytic potential. A number of indigenously isolated fungi were screened for cellulytic potential. In the present study, the kinetics of cellulase production from an indigenous strain of Aspergillus niger MS82 is reported. Product formation parameters of endoglucanase and beta-glucosidase (Qp + Y(p/s)) indicate that A.niger MS82 is capable of producing moderate to high levels of both endoglucanase and beta-glucosidase when grown on different carbon containing natural substrates, for example, grass, corncob, bagasse along side purified celluloses. Furthermore, it was observed that the production of endoglucanase reaches its maximum during exponential phase of growth, while b-glucosidase during the Stationary phase. Enzyme production by solid-state fermentation was also investigated and found to be promising.Highest production of cellulase was noted at pH 4.0 at 35 degrees C under submerged conditions. Growth and enzyme production was affected by variations in temperature and pH.
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Cellulase has an optimal temperature of 30-45 • C and an optimal pH of 4.5-5 and is applicable to macroalgae.
“Cellulase | | 30-45 • C | 4.5-5 | Macroalgae | [216]”
From A Review of Algae-Based Carbon Capture, Utilization, and Storage (Algae-Based CCUS) (2024) · cites this paper as [216]
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Self-citation
- Authors
- Sohail, M., Siddiqi, R., Ahmad, A., & Khan, S. A.
- Affiliations
- University of Karachi (PK)
- Corresponding author
- Khan, Shakeel Ahmed
- Venue
- New Biotechnology
- Publisher
- Elsevier BV
- Published
- 2009-09
- Volume
- 25
- Issue
- 6
- Pages
- 437-441
- ISSN
- 1871-6784 (ISSN-L)
- OpenAlex
- W2012822513
- Cited by
- 230
- References
- 29
- Citations by year
- 2026: 8 · 2025: 16 · 2024: 8 · 2023: 18 · 2022: 15 · 2021: 13 · 2020: 18 · 2019: 18 · 2018: 19 · 2017: 12 · 2016: 16 · 2015: 11 · 2014: 9 · 2013: 15 · 2012: 7
- FWCI
- 9.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- New Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- cellulase production, Aspergillus niger, endoglucanase, β-glucosidase, temperature effects, pH effect, solid-state fermentation, cellulose hydrolysis, corn cob, sugarcane bagasse, Poaceae, enzyme kinetics
- MeSH terms
- Aspergillus niger, Cellulase, Hydrogen-Ion Concentration, Temperature, Cell Culture Techniques, Bioreactors
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