CoChat
Sign Up
Cited by 83Neuroscience and Neuropharmacology ResearchCellular transport and secretionProtein Kinase Regulation and GTPase Signaling

IQ-ArfGEF/BRAG1 is a guanine nucleotide exchange factor for Arf6 that interacts with PSD-95 at postsynaptic density of excitatory synapses.

Sakagami, Hiroyuki, Sanda, Masashi, Fukaya, Masahiro, Miyazaki, Taisuke, Sukegawa, Jun, Yanagisawa, Teruyuki, Suzuki, Tatsuo, Fukunaga, Kohji +2 more·2008·Neuroscience Research
DOI 10.1016/j.neures.2007.10.013
Open this paper in CoChat — free account

Abstract

ADP ribosylation factor 6 (Arf6) is a small GTPase that regulates dendritic differentiation possibly through the organization of actin cytoskeleton and membrane traffic. Here, we characterized IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor (GEF) for Arf6, in the mouse brain. In vivo Arf pull down assay demonstrated that IQ-ArfGEF/BRAG1 activated Arf6 more potently than Arf1. IQ-ArfGEF/BRAG1 mRNA was abundantly expressed in the brain with higher levels in forebrain structures and cerebellar granule cells. In hippocampal neurons, IQ-ArfGEF/BRAG1 mRNA was localized not only at neuronal cell bodies but also at dendritic processes, indicating its dendritic transport and localization. Immunoprecipitation and in vitro binding experiments revealed that IQ-ArfGEF/BRAG1 formed a protein complex with N-methyl-d-aspartate (NMDA)-type glutamate receptors through the interaction with a postsynaptic density (PSD) scaffold protein, PSD-95. Immunohistochemical analysis demonstrated that IQ-ArfGEF/BRAG1 was localized preferentially at the postsynaptic density of asymmetrical synapses on dendritic spines, but was lacking at GABAa receptor-carrying inhibitory synapses. Taken together, IQ-ArfGEF/BRAG1 forms a postsynaptic protein complex containing PSD-95 and NMDA receptors at excitatory synapses, where it may function as a GEF for Arf6.

Integrity signals

Registry

Crossref · 10.1016/j.neures.2007.10.013

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Members of the ARF family of small GTPases and their regulators are important regulators of spine density and morphology.

    “Members of the ADP-ribosylation factor (ARF) family of small GTPases and their regulators have gained increasing attention as important regulators of spine density and morphology [27], [28], [29].”

    From Arf4 Determines Dentate Gyrus-Mediated Pattern Separation by Regulating Dendritic Spine Development (2012) · cites this paper as [28]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Sakagami, H., Sanda, M., Fukaya, M., Miyazaki, T., Sukegawa, J., Yanagisawa, T., Suzuki, T., Fukunaga, K., Watanabe, M., & Kondo, H.
Affiliations
  • Tohoku University (JP)
  • Hokkaido University (JP)
  • Shinshu University (JP)
Corresponding author
Sakagami, Hiroyuki
ORCID
Venue
Neuroscience Research
Publisher
Elsevier BV
Published
2008-02
Volume
60
Issue
2
Pages
199-212
ISSN
0168-0102 (ISSN-L)
DOI
10.1016/j.neures.2007.10.013
OpenAlex
W2054545874
Cited by
83
References
1
Citations by year
2026: 3 · 2025: 6 · 2024: 4 · 2023: 6 · 2022: 3 · 2021: 6 · 2020: 5 · 2019: 7 · 2018: 3 · 2017: 2 · 2016: 12 · 2015: 4 · 2014: 1 · 2013: 3 · 2012: 6
FWCI
1.38
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Neuroscience Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Education, Culture, Sports, Science and Technology
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
ARF6, guanine nucleotide exchange factor, PSD-95, postsynaptic density, excitatory synapses, NMDA receptor, dendritic spines, dendritic transport, hippocampal neurons, cerebellar granule cells, small GTPase activation, asymmetrical synapses, inhibitory synapses
MeSH terms
Disks Large Homolog 4 Protein, ADP-Ribosylation Factor 6, Animals, Brain, Chlorocebus aethiops, Cloning, Molecular, Guinea Pigs, Membrane Proteins, Mice, Inbred C57BL, Neurons, Rabbits, Receptors, GABA-A, Synapses, Transfection, Receptors, N-Methyl-D-Aspartate, Gene Expression Regulation, Developmental, COS Cells, Guanine Nucleotide Exchange Factors, ADP-Ribosylation Factors, Electrophoretic Mobility Shift Assay, Immunoprecipitation, Intracellular Signaling Peptides and Proteins, Mice, Guanylate Kinases

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review