Anomalous Diffusion in Purkinje Cell Dendrites Caused by Spines
Open accessAbstract
We combined local photolysis of caged compounds with fluorescence imaging to visualize molecular diffusion within dendrites of cerebellar Purkinje cells. Diffusion of a volume marker, fluorescein dextran, within spiny dendrites was remarkably slow in comparison to its diffusion in smooth dendrites. Computer simulations indicate that this retardation is due to a transient trapping of molecules within dendritic spines, yielding anomalous diffusion. We considered the influence of spine trapping on the diffusion of calcium ions (Ca(2+)) and inositol-1,4,5-triphospate (IP(3)), two synaptic second messengers. Diffusion of IP(3) was strongly influenced by the presence of dendritic spines, while Ca(2+) was removed so rapidly that it could not diffuse far enough to be trapped. We conclude that an important function of dendritic spines may be to trap chemical signals and thereby create slowed anomalous diffusion within dendrites.
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BDNF action was selective on the spines that showed immediate enlargement, which may act as the structural tag for selective trapping of the protein-synthetic machinery and the capture of plasticity proteins for long-term spine-head enlargement.
“BDNF action was selective on the spines that showed immediate enlargement, which may act as the structural tag for selective trapping (26) of the protein-synthetic machinery (11, 27) and the capture of plasticity proteins (2, 28) for long-term spine-head enlargement.”
From Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines (2008) · cites this paper as [26]
Cites
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Synaptic tagging and long-term potentiation
Frey, U., & Morris, R. G. M. (1997) · Nature · doi:10.1038/385533a0 · Cited by 1,736 · cited 2× · see citing works
“This could contribute to ‘‘synaptic tagging,’’ the selective trapping of dendritic molecules by active spines that may be required for long-term plasticity (Frey and Morris, 1997).”
BackgroundShrinkage of dendritic spines associated with long-term depression of hippocampal synapses
Zhou, Q., Homma, K. J., & Poo, M.-M. (2004) · Neuron · doi:10.1016/j.neuron.2004.11.011 · Cited by 1,025 · see citing works
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Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines
Tanaka, J.-I., Horiike, Y., Matsuzaki, M., Miyazaki, T., Ellis-Davies, G. C. R., & Kasai, H. (2008) · Science · doi:10.1126/science.1152864 · Cited by 615 · see citing works
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Can we detect the effect of spines and leaflets on the diffusion of brain intracellular metabolites?
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The impacts of geometry and binding on CaMKII diffusion and retention in dendritic spines
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- Authors
- Santamaria, F., Wils, S., De Schutter, E., & Augustine, G. J.
- Affiliations
- University of Antwerp (BE)
- Duke Medical Center (US)
- Corresponding author
- Augustine, George J.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2006-11
- Volume
- 52
- Issue
- 4
- Pages
- 635-648
- ISSN
- 0896-6273 (ISSN-L)
- PMC
- PMC1994115
- OpenAlex
- W2156961733
- Cited by
- 279
- References
- 5
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 10 · 2023: 13 · 2022: 11 · 2021: 10 · 2020: 14 · 2019: 15 · 2018: 22 · 2017: 21 · 2016: 11 · 2015: 21 · 2014: 22 · 2013: 25 · 2012: 13
- FWCI
- 4.55
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of General Medical Sciences (GM 65473, R24GM065473)
- National Institute of Neurological Disorders and Stroke (R01NS034045, NS34045)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Physical Sciences › Mathematics › Modeling and Simulation › Fractional Differential Equations Solutions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- anomalous diffusion, dendritic spines, Purkinje cell dendrites, cerebellum, calcium diffusion, inositol 1,4,5-trisphosphate, fluorescein dextran, dendritic transport
- MeSH terms
- Animals, Calcium, Cerebellar Cortex, Diffusion, Models, Molecular, Synaptic Transmission, Organ Culture Techniques, Purkinje Cells, Synaptic Membranes, Second Messenger Systems, Signal Transduction, Inositol 1,4,5-Trisphosphate, Fluorescein, Dendritic Spines, Mice
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