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Transient expansion of synaptically connected dendritic spines upon induction of hippocampal long-term potentiation.

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Lang, Cynthia, Barco, Angel, Zablow, Leonard, Kandel, Eric R., Siegelbaum, Steven A., Zakharenko, Stanislav S.·2004·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0407581101PMC PMC534531
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Abstract

Dendritic spines are small protrusions from dendritic shafts that contain the postsynaptic sites of glutamatergic synapses in the brain. Spines undergo dramatic activity-dependent structural changes that are particularly prominent during neuronal development. Although changes in spine shape or number have been proposed to contribute to forms of synaptic plasticity that underlie learning and memory, the extent to which spines remain plastic in the adult brain is unclear. We find that induction of long-term potentiation (LTP) of synaptic transmission in acute hippocampal slices of adult mice evokes a reliable, transient expansion in spines that are synaptically activated, as determined with calcium imaging. Similar to LTP, transient spine expansion requires N-methyl-D-aspartate (NMDA) receptor-mediated Ca2+ influx and actin polymerization. Moreover, like the early phase of LTP induced by the stimulation protocol, spine expansion does not require Ca2+ influx through L-type voltage-gated Ca2+ channels nor does it require protein synthesis. Thus, transient spine expansion is a characteristic feature of the initial phases of plasticity at mature synapses and so may contribute to synapse remodeling important for LTP.

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Authors
Lang, C., Barco, A., Zablow, L., Kandel, E. R., Siegelbaum, S. A., & Zakharenko, S. S.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Center for Neurobiology and Behavior, Departments of Pharmacology and Physiology and Biophysics, Kavli Institute for Brain Sciences, and Howard Hughes Medical Institute, Columbia University, 1051 Riverside Drive, New York, NY 10032
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2004-11-12
Volume
101
Issue
47
Pages
16665-16670
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0407581101
PMC
PMC534531
OpenAlex
W2082900258
Cited by
245
References
34
Citations by year
2026: 2 · 2025: 5 · 2024: 4 · 2023: 7 · 2022: 6 · 2021: 8 · 2020: 11 · 2019: 16 · 2018: 10 · 2017: 7 · 2016: 7 · 2015: 8 · 2014: 16 · 2013: 14 · 2012: 10
FWCI
5.52
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
Keywords
dendritic spines, long-term potentiation, hippocampus, synaptic plasticity, NMDA receptor, calcium influx, actin polymerization, L-type calcium channels, protein synthesis, synaptic remodeling, adult brain plasticity
MeSH terms
Animals, Dendrites, Electric Stimulation, Hippocampus, Mice, Inbred C57BL, Mice, Transgenic, Synaptic Transmission, Recombinant Proteins, Synapses, Long-Term Potentiation, Pyramidal Cells, Calcium Signaling, Calcium Channels, L-Type, Green Fluorescent Proteins, Mice, In Vitro Techniques

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