The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Single-Synapse Analysis of a Diverse Synapse Population: Proteomic Imaging Methods and Markers
Open accessAbstract
Summary A lack of methods for measuring the protein compositions of individual synapses in situ has so far hindered the exploration and exploitation of synapse molecular diversity. Here we describe the use of array tomography, a new high-resolution proteomic imaging method, to determine the composition of glutamate and GABA synapses in somatosensory cortex of Line-H-YFP Thy-1 transgenic mice. We find that virtually all synapses are recognized by antibodies to the presynaptic phosphoprotein synapsin I, while antibodies to 16 other synaptic proteins discriminate amongst 4 subtypes of glutamatergic synapses and GABAergic synapses. Cell-specific YFP expression in the YFP-H mouse line allows synapses to be assigned to specific presynaptic and postsynaptic partners and reveals that a subpopulation of spines on layer 5 pyramidal cells receives both VGluT1-subtype glutamatergic and GABAergic synaptic inputs. These results establish a means for the high-throughput acquisition of proteomic data from individual cortical synapses in situ.
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Rapid formation and selective stabilization of synapses for enduring motor memories
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Stably maintained dendritic spines are associated with lifelong memories
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Self-citationSynapsins as Regulators of Neurotransmitter Release
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Experience leaves a lasting structural trace in cortical circuits
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Reconstruction and Simulation of Neocortical Microcircuitry
Markram, H., Muller, E., Ramaswamy, S., Reimann, M. W., Abdellah, M., Sanchez, C. A., Ailamaki, A., Alonso-Nanclares, L., Antille, N., Arsever, S., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.029 · Cited by 1,557
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Paşca, A. M., Sloan, S. A., Clarke, L. E., Tian, Y., Makinson, C. D., Huber, N., Kim, C. H., Park, J.-Y., O'Rourke, N. A., Nguyen, K. D., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3415 · Cited by 1,609
Self-citationChan, K. Y., Jang, M. J., Yoo, B. B., Greenbaum, A., Ravi, N., Wu, W.-L., Sánchez-Guardado, L., Lois, C., Mazmanian, S. K., Deverman, B. E., et al. (2017) · Nature Neuroscience · doi:10.1038/nn.4593 · Cited by 1,578
Astrocytes mediate synapse elimination through MEGF10 and MERTK pathways
Chung, W.-S., Clarke, L. E., Wang, G. X., Stafford, B. K., Sher, A., Chakraborty, C., Joung, J., Foo, L. C., Thompson, A., Chen, C., et al. (2013) · Nature · doi:10.1038/nature12776 · Cited by 1,377
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Metadata
- Authors
- Micheva, K. D., Busse, B., Weiler, N. C., O'Rourke, N., & Smith, S. J.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2010-11
- Volume
- 68
- Issue
- 4
- Pages
- 639-653
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2024015768
- Cited by
- 336
- References
- 7
- FWCI
- 10.65
- Type
- journal article
- Language
- en
- Open access
- bronze
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