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Cited by 124Neuroscience and Neuropharmacology ResearchPhotoreceptor and optogenetics researchAdvanced Fluorescence Microscopy Techniques

Optogenetic Control of Synaptic Composition and Function

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Sinnen, Brooke L., Bowen, Aaron B., Forte, Jeffrey S., Hiester, Brian G., Crosby, Kevin C., Gibson, Emily S., Dell’Acqua, Mark L., Kennedy, Matthew J.·2017·Neuron
DOI 10.1016/j.neuron.2016.12.037PMC PMC5300939
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Abstract

The molecular composition of the postsynaptic membrane is sculpted by synaptic activity. During synaptic plasticity at excitatory synapses, numerous structural, signaling, and receptor molecules concentrate at the postsynaptic density (PSD) to regulate synaptic strength. We developed an approach that uses light to tune the abundance of specific molecules in the PSD. We used this approach to investigate the relationship between the number of AMPA-type glutamate receptors in the PSD and synaptic strength. Surprisingly, adding more AMPA receptors to excitatory contacts had little effect on synaptic strength. Instead, we observed increased excitatory input through the apparent addition of new functional sites. Our data support a model where adding AMPA receptors is sufficient to activate synapses that had few receptors to begin with, but that additional remodeling events are required to strengthen established synapses. More broadly, this approach introduces the precise spatiotemporal control of optogenetics to the molecular control of synaptic function.

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  • Activity-dependent synaptic recruitment of AMPARs may involve two steps: delivery to the PSD, then addition to AMPAR clusters in the nanocolumn.

    “It is suggested that activity-dependent synaptic recruitment of AMPARs may involve two steps (259, 261). First, AMPARs are delivered to the PSD, but this is not enough to increase synaptic strength. Second, the newly recruited AMPARs are added to AMPAR clusters in the nanocolumn.”

    From Synaptic memory and CaMKII (2023) · cites this paper as [261]

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Cited by

Sorted by citations

Authors
Sinnen, B. L., Bowen, A. B., Forte, J. S., Hiester, B. G., Crosby, K. C., Gibson, E. S., Dell’Acqua, M. L., & Kennedy, M. J.
Affiliations
University of Colorado Anschutz (US)
Corresponding author
Kennedy, Matthew J.
ORCID
Venue
Neuron
Publisher
Elsevier BV
Published
2017-02
Volume
93
Issue
3
Pages
646-660.e5
ISSN
0896-6273 (ISSN-L)
DOI
10.1016/j.neuron.2016.12.037
PMC
PMC5300939
OpenAlex
W2582950460
Cited by
124
References
10
Citations by year
2026: 4 · 2025: 8 · 2024: 7 · 2023: 14 · 2022: 9 · 2021: 16 · 2020: 16 · 2019: 13 · 2018: 26 · 2017: 11
FWCI
8.84
Type
journal article
Language
en
Open access
Licence
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • The McKnight Foundation (T32AA007464)
  • The Pew Charitable Trusts (T32HD041697)
  • The Brain Research Foundation (NS040701, T32GM007635)
  • The Brain and Behavior Research Foundation (NS092421)
  • The Boettcher Foundation (NS082271)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Keywords
optogenetics, postsynaptic density, AMPA receptors, synaptic plasticity, synaptic efficacy, excitatory synapses, synaptic remodeling
MeSH terms
Animals, Hippocampus, Neuronal Plasticity, Neurons, Synapses, Synaptic Membranes, Rats, Sprague-Dawley, Long-Term Potentiation, Receptors, AMPA, Patch-Clamp Techniques, Arabidopsis Proteins, Rats, Basic Helix-Loop-Helix Transcription Factors, Basic Helix-Loop-Helix Proteins, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Cryptochromes, Post-Synaptic Density, Optogenetics

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