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Comparative distribution of nicotinic receptor subtypes during development, adulthood and aging: an autoradiographic study in the rat brain
Open accessAbstract
The distribution in the rat brain of high affinity nicotinic heteromeric acetylcholine receptors and of low affinity nicotinic, alpha7-containing, homomeric receptors was studied using in vitro light microscopic autoradiography. As ligands, we used [3H]epibatidine, or [125I]epibatidine, and [125I]alpha-bungarotoxin, respectively. In adult animals, the two types of binding sites were widely distributed in many different brain structures, including the brainstem, cerebellum, mesencephalic structures, limbic system and cortex, but their anatomical distribution differed markedly. Only in rare instances could a co-localization be observed, for example in the superficial layer of the superior colliculus. In developing animals, both types of labeling were strongly expressed during embryonic and postnatal phases. Their distributions were qualitatively similar to those observed in adult animals, with a few noticeable exceptions in the cerebral cortex, hippocampus and brain stem. In aging animals, neither the distribution nor the density of nicotinic binding sites was significantly altered. Our conclusions are the following. (a) There is little overlap in the distribution of heteromeric and alpha7-containing homomeric nicotinic receptors in the rat brain. (b) The abundance of neuronal nicotinic receptors during embryonic and postnatal development suggests that they may play a role in the establishment of neuronal connectivity. (c) The expression of neuronal nicotinic receptors is unaltered in middle aged animals, suggesting that in the rat these receptors do not play any major role in aging process.
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“Receptors, transporters as well as synthesizing enzymes are already expressed (~E10 rodent and week 4–5 human) even when the actual axonal projections have not yet arrived in the PFC [236–240].”
From Development of prefrontal cortex (2022) · cites this paper as [239]
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Metadata
- Authors
- Tribollet, E., Bertrand, D., Marguerat, A., & Raggenbass, M.
- Affiliations
- University of Geneva (CH)
- ORCID
- Bertrand, D
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 2004-01
- Volume
- 124
- Issue
- 2
- Pages
- 405-420
- ISSN
- 0306-4522 (ISSN-L)
- OpenAlex
- W2140445474
- Cited by
- 199
- References
- 64
- Citations by year
- 2025: 3 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 6 · 2020: 4 · 2019: 8 · 2018: 7 · 2017: 6 · 2016: 3 · 2015: 13 · 2014: 8 · 2013: 9 · 2012: 18
- FWCI
- 5.80
- Type
- journal article
- Language
- en
- Open access
- Licence
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Smoking Behavior and Cessation
- Keywords
- nicotinic acetylcholine receptors, alpha7 nicotinic acetylcholine receptor, receptor distribution, brain development, aging, embryonic development, postnatal development, receptor binding sites, epibatidine binding, alpha-bungarotoxin binding, neuronal connectivity, rat brain
- MeSH terms
- Age Factors, Aging, Animals, Animals, Newborn, Autoradiography, Binding Sites, Brain, Brain Chemistry, Bungarotoxins, Embryo, Mammalian, Female, Iodine Isotopes, Male, Pyridines, Receptors, Nicotinic, Tissue Distribution, Tritium, Rats, Sprague-Dawley, Rats, Wistar, Nicotinic Agonists, Bridged Bicyclo Compounds, Heterocyclic, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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