CoChat
Sign Up
Cited by 291Nitric Oxide and Endothelin EffectsRedox biology and oxidative stressNeutrophil, Myeloperoxidase and Oxidative Mechanisms

The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.

Neuronal expression of the NADPH oxidase NOX4, and its regulation in mouse experimental brain ischemia

Open access
Vallet, P., Charnay, Y., Steger, K., Ogier-Denis, E., Kovari, E., Herrmann, F., Michel, J.-P., Szanto, I.·2005·Neuroscience
DOI 10.1016/j.neuroscience.2004.12.038
Open this paper in CoChat — free account

Abstract

Ischemia-induced neuronal damage has been linked to elevated production of reactive oxygen species (ROS) both in animal models and in humans. NADPH oxidase enzymes (NOX-es) are a major enzymatic source of ROS, but their role in brain ischemia has not yet been investigated. The present study was carried out to examine the expression of NOX4, one of the new NADPH oxidase isoforms in a mouse model of focal permanent brain ischemia. We demonstrate that NOX4 is expressed in neurons using in situ hybridization and immunohistochemistry. Ischemia, induced by middle cerebral artery occlusion resulted in a dramatic increase in cortical NOX4 expression. Elevated NOX4 mRNA levels were detectable as early as 24 h after the onset of ischemia and persisted throughout the 30 days of follow-up period, reaching a maximum between days 7 and 15. The early onset suggests neuronal reaction, while the peak period corresponds to the time of neoangiogenesis occurring mainly in the peri-infarct region. The occurrence of NOX4 in the new capillaries was confirmed by immunohistochemical staining. In summary, our paper reports the presence of the ROS producing NADPH oxidase NOX4 in neurons and demonstrates an upregulation of its expression under ischemic conditions. Moreover, a role for NOX4 in ischemia/hypoxia-induced angiogenesis is suggested by its prominent expression in newly formed capillaries.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.neuroscience.2004.12.038

Cites

51 of 51 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 50 references

Research works printed but not linked: 2

  1. Saran, 2003, To what end does nature produce superoxide?, NADPH oxidase as an autocrine modifier of membrane phospholipids generating paracrine lipid messengers. Free Radic Res, 37, 1045
  2. Soberman, 2003, The expanding network of redox signaling, J Clin Invest, 111, 571, 10.1172/JCI18099 · DOI

Cited by

Sorted by citations

See all 291

Related

Metadata

Authors
Vallet, P., Charnay, Y., Steger, K., Ogier-Denis, E., Kovari, E., Herrmann, F., Michel, J.-P., & Szanto, I.
Affiliations
University of Geneva (CH)
ORCID
Venue
Neuroscience
Publisher
Elsevier BV
Published
2005-01
Volume
132
Issue
2
Pages
233-238
ISSN
0306-4522 (ISSN-L)
DOI
10.1016/j.neuroscience.2004.12.038
OpenAlex
W1971556924
Cited by
291
References
50
Citations by year
2026: 4 · 2025: 5 · 2024: 7 · 2023: 11 · 2022: 8 · 2021: 10 · 2020: 10 · 2019: 10 · 2018: 13 · 2017: 19 · 2016: 14 · 2015: 11 · 2014: 21 · 2013: 14 · 2012: 26
FWCI
9.79
Type
journal article
Language
en
Open access
Licence
Locations
  • Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
Topics
  • Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
NOX4, NADPH oxidase, cerebral ischemia, focal cerebral ischemia, middle cerebral artery occlusion, reactive oxygen species, neuronal expression, ischemic neuronal injury, gene upregulation
MeSH terms
NADPH Oxidase 4, Animals, Brain Ischemia, Disease Models, Animal, Immunohistochemistry, Kidney, Functional Laterality, Mice, Inbred C57BL, Neurons, RNA, Messenger, Time Factors, Blotting, Northern, Blotting, Western, Gene Expression Regulation, Enzymologic, In Situ Hybridization, NADPH Oxidases, Reverse Transcriptase Polymerase Chain Reaction, Infarction, Middle Cerebral Artery, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review