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Cited by 16Neuroscience and Neuropharmacology ResearchNeuropeptides and Animal PhysiologyHeart Rate Variability and Autonomic Control

Phosphorylated extracellular signal-regulated kinase 1/2 immunoreactivity identifies a novel subpopulation of sympathetic preganglionic neurons

Springell, D.A., Powers-Martin, K., Phillips, J.K., Pilowsky, P.M., Goodchild, A.K.·2005·Neuroscience
DOI 10.1016/j.neuroscience.2005.02.036
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Abstract

Distinct chemical codes are thought to reflect functional specificity in sympathetic preganglionic neurons (SPN). Although a number of chemical candidates have been identified including neurotransmitter-related, calcium-binding and other proteins, signal transduction proteins have been largely neglected. Not only might these chemicals allow discrimination of functionally unique chemical signatures, but they may also identify activated neurons. Immunoreactivity (ir) to phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2) was differentially located within the thoracic spinal cord depending upon which of three forms of killing was used: the only exception to this was the intermediolateral cell column (IML) which was consistently, densely labeled. The presence or absence of p-ERK1/2 in SPN (n=17,541) within the IML of the thoraco-lumbar spinal cord was determined in seven rats. SPN were identified on the basis of their location, size and that they contained choline acetyltransferase ir. On average, 58% of SPN contained p-ERK1/2, however, more SPN in both the upper (72%; C8-T4) and lower (78%; T11-L3) thoraco-lumbar spinal cord contained p-ERK1/2-ir than the middle thoracic region (47%; T4-T10). p-ERK1/2-ir was also examined in SPN (n=1895) innervating the adrenal medulla (identified by retrograde tracing using cholera toxin B subunit) combined with localization of neuronal nitric oxide synthase (nNOS) in three rats. On average, 64% of adrenal SPN contain p-ERK1/2-ir, and it was confirmed that all adrenal SPN contain nNOS-ir. It appears that p-ERK1/2-ir SPN, described in this study, have tonically activated receptors that are coupled to intracellular signal transduction pathways that lead to the phosphorylation of ERK1/2.

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  • The motoneuron family includes alpha motoneurons (MNa) with relatively higher levels of Poln and Spp134, gamma motoneurons (MNg) with relatively higher levels of Esrrg and Htr1f, and preganglionic cells (PGC) expressing Gfra3, Nos1, and Fbn2.

    “The motoneuron (MN) family includes alpha motoneurons (MNa) which had relatively higher levels of Poln and Spp134, gamma motoneurons (MNg) which had relatively higher levels of Esrrg and Htr1f35, and the related preganglionic cells (PGC) which expressed Gfra3, Nos1, and Fbn236,37.”

    From A harmonized atlas of mouse spinal cord cell types and their spatial organization (2021) · cites this paper as [37]

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Authors
Springell, D. A., Powers-Martin, K., Phillips, J. K., Pilowsky, P. M., & Goodchild, A. K.
Affiliations
  • The University of Sydney (AU)
  • Murdoch University (AU)
ORCID
Venue
Neuroscience
Publisher
Elsevier BV
Published
2005-01
Volume
133
Issue
2
Pages
583-590
ISSN
0306-4522 (ISSN-L)
DOI
10.1016/j.neuroscience.2005.02.036
OpenAlex
W1973695071
Cited by
16
References
44
Citations by year
2021: 1 · 2016: 2 · 2015: 2 · 2013: 1 · 2012: 1
FWCI
0.60
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Murdoch University Research Portal (submittedVersion)
Funders
Royal North Shore Hospital
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Heart Rate Variability and Autonomic Control
Keywords
ERK1/2 phosphorylation, sympathetic preganglionic neurons, signal transduction proteins, intermediolateral cell column, neuronal nitric oxide synthase, choline acetyltransferase
MeSH terms
Animals, Autonomic Fibers, Preganglionic, Cell Count, Cholera Toxin, Choline O-Acetyltransferase, Halothane, Immunohistochemistry, Male, Nerve Tissue Proteins, Neurons, Pentobarbital, Phosphorylation, Spinal Cord, Rats, Sprague-Dawley, Nitric Oxide Synthase, Intracellular Signaling Peptides and Proteins, Rats, Nitric Oxide Synthase Type I

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