Influence and mechanism of 5-aminolevulinic acid-photodynamic therapy on the metastasis of esophageal carcinoma
Abstract
Backgroud Photodynamic therapy (PDT) for the treatment of esophageal cancer was more and more popularly used since it was approved for the treatment of advanced esophageal cancer in 1996. It has been reported to influence the tumor growth and metastasis via a variety of signaling pathways, but its mechanism remains to be further studied. This research studied the effects of ALA-PDT on esophageal carcinoma in vitro and in vivo, discovering its molecular regulating mechanism and the way to enhence the PDT effect. Methods Eca-109 cells were incubated with a medium containing EGFR tyrphostin AG1478 or PI3K inhibitor LY294002, then with ALA, and the cells were irradiated with the laser 6h later. The cell viability was measured with MTT assay, and the migration ability was detected by transwell experiments 24h post-ALA-PDT. The gene and protein expression on EGFR/PI3K/AKT signaling pathway was analyzed by realtime PCR and Western blotting respectively. Then, RFP-Eca-109 burdened nude mice model was constructed, and were treated with ALA-PDT when the tumor volume reached 150-350mm 3. The gene and protein expression were analyzed 24h and 50days post-ALA-PDT. Results Our study showed that ALA-PDT respectively combined with AG1478, LY294002 could synergistically reduce the growth and migration ability of the Eca-109 cells in vitro and significantly down-regulate the protein expression of EGFR/PI3K and PI3K/AKT, meanwhile, significantly down-regulate the gene expression of EGFR when combining with AG1478. Forthermore, ALA-PDT could significantly decrease the tumor growth and metastasis and down-regulate the gene expression of EGFR and the protein expression of EGFR and PI3K in the tumor of mice. Conclusion This study revealed a molecular mechanism of ALA-PDT and developed a new modality application of therapy, by combining ALA-PDT with small molecular inhibitors, for better effect in the clinical practice of esophageal carcinoma.
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Targeting these pro-survival signaling pathways has been shown to enhance ALA-PDT response.
“Targeting these pro-survival signaling pathways has been shown to enhance ALA-PDT response.”
From 5-Aminolevulinic Acid as a Theranostic Agent for Tumor Fluorescence Imaging and Photodynamic Therapy (2023) · cites this paper as [138]
Targeting the PI3K pathway with inhibitor LY294002 significantly enhances PDT outcomes in esophageal tumor cells and tumor model.
“and targeting the PI3K pathway with inhibitor LY294002 significantly enhances PDT outcomes in esophageal tumor cells and tumor model [138].”
From 5-Aminolevulinic Acid as a Theranostic Agent for Tumor Fluorescence Imaging and Photodynamic Therapy (2023) · cites this paper as [138]
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Retracted
- Authors
- Zhang, X., Cai, L., He, J., Li, X., Li, L., Chen, X., & Lan, P.
- Affiliations
- Sixth Affiliated Hospital of Sun Yat-sen University (CN)
- Nanfang Hospital (CN)
- TCM-Intigrated Cancer Center of Southern Medical University (CN)
- Panyu District Central Hospital (CN)
- Corresponding author
- Li, Libo
- Chen, Xiaohua
- Lan, Ping
- Venue
- Photodiagnosis and Photodynamic Therapy
- Publisher
- Elsevier BV
- Published
- 2017-12
- Volume
- 20
- Pages
- 78-85
- ISSN
- 1572-1000 (ISSN-L)
- OpenAlex
- W2747891421
- Cited by
- 14
- References
- 55
- Citations by year
- 2025: 2 · 2023: 5 · 2022: 3 · 2021: 1 · 2019: 2 · 2018: 1
- FWCI
- 0.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Photodiagnosis and Photodynamic Therapy (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Technical New Star of Zhujiang, Pan Yu district, Guangzhou (2013-special-15-6.10)
- Traditional Chinese Medicine Bureau of Guangdong Province (20161186)
- Natural science foundation of Guangdong province (s2011040000786)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Health Sciences › Medicine › Surgery › Esophageal Cancer Research and Treatment
- Keywords
- 5-aminolevulinic acid, photodynamic therapy, esophageal cancer, metastasis, EGFR, PI3K/Akt signaling, AG1478, LY294002, combination therapy, Eca-109 cells, tumor cell migration, tumor growth inhibition
- MeSH terms
- Aminolevulinic Acid, Animals, Cell Survival, Chromones, Drug Therapy, Combination, Esophageal Neoplasms, Female, Male, Mice, Inbred BALB C, Mice, Nude, Morpholines, Photochemotherapy, Protein Kinases, Quinazolines, Signal Transduction, Down-Regulation, Apoptosis, Photosensitizing Agents, Tyrphostins, Cell Line, Tumor, Mice
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