Neuropeptide Y conjugated to saporin alters anxiety-like behavior when injected into the central nucleus of the amygdala or basomedial hypothalamus in BALB/cJ mice
Open accessAbstract
Neuropeptide Y (NPY) is a 36-amino-acid neuromodulator that is distributed throughout the central nervous system and has been implicated in a wide range of neurobiological responses including the integration of emotional behavior. The anxiolytic properties of NPY are modulated by NPY signaling in the hippocampus and in the central (CeA) and basolateral (BLA) nuclei of the amygdala. Recently, the neurotoxin saporin, when conjugated to NPY (NPY-SAP), was shown to selectively kill NPY receptor-expressing neurons and has been used as a tool to study the central NPY neurocircuitry involved with feeding behaviors. Here we determined if NPY-SAP can be used as a tool to study the central NPY neurocircuitry that modulates anxiety-like behaviors. BALB/cJ mice were given injection of either NPY-SAP or a control blank saporin (B-SAP) into the CeA or the basomedial hypothalamus (BMH) as a control injection site. The elevated zero maze test was used to assess anxiety-like behavior and NPY-SAP-induced lesions were verified using NPY Y1 receptor (Y1R) immunoreactivity (IR). Results showed that injection of NPY-SAP into the CeA site-specifically blunted Y1R IR in the CeA which was associated with a significant increase in anxiety-like behavior. Injection of NPY-SAP into the BMH, while locally blunting Y1R IR, promoted a compensatory increase of Y1R IR in the BLA and the CA3 region of the hippocampus which was associated with a significant reduction of anxiety-like behavior. The present set of experiments suggest that the NPY-SAP neurotoxin may be a useful tool for studying the NPY neurocircuitry that modulates anxiety-like behaviors.
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The findings are consistent with prior reports implicating the CeA in anxiety.
“These data are consistent with reports implicating CeA involvement in anxiety9,11,12”
From Amygdala circuitry mediating reversible and bidirectional control of anxiety (2011) · cites this paper as [12]
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- Authors
- Lyons, A. M., & Thiele, T. E.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- ORCID
- Thiele, Todd E.
- Venue
- Peptides
- Publisher
- Elsevier BV
- Published
- 2010-12
- Volume
- 31
- Issue
- 12
- Pages
- 2193-2199
- ISSN
- 0196-9781 (ISSN-L)
- PMC
- PMC2971693
- OpenAlex
- W1972603427
- Cited by
- 21
- References
- 31
- Citations by year
- 2024: 1 · 2020: 1 · 2019: 1 · 2018: 4 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 4
- FWCI
- 1.03
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Peptides (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Carolina Digital Repository (University of North Carolina at Chapel Hill) (submittedVersion)
- UNC Libraries
- Funders
- U.S. Department of Defense (W81XWH0610158, W81XWH, W81XWH0910293)
- National Institutes of Health (W81XWH, AA013573)
- National Institute on Alcohol Abuse and Alcoholism (R01AA015148, AA013573, F31AA017818, AA015148, R01AA013573, AA017818, R37AA013573)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- neuropeptide Y, anxiety-like behavior, central amygdala, NPY Y1 receptor, hippocampal CA3, elevated zero maze, BALB/cJ mice
- MeSH terms
- Saporins, Amygdala, Animals, Anxiety, Hypothalamus, Immunohistochemistry, Male, Mice, Inbred BALB C, Neuropeptide Y, Anti-Anxiety Agents, Maze Learning, Mice, Ribosome Inactivating Proteins, Type 1
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